Biomedical subjects

Jirí Hasek

Publications and source records attributed to Jirí Hasek.

3 recordsLinked to original sources

PubMed2006

The WD-40 repeat protein PkwA of Thermomonospora curvata is associated with rapid growth and is localized in the tips of growing hyphae.

The PkwA protein of the thermophilic actinomycete Thermomonospora curvata has already been reported as the first instance of a WD-40 module-containing protein of prokaryotic origin. This protein is composed of an N-terminal eukaryotic-type protein kinase domain and of seven C-terminal WD-40 repeats. PkwA is a peripheral membrane protein that is linked to the early exponential growth phase of the bacterium. Its intracellular concentrations are extremely low. We have shown that the protein forms high molecular weight complexes and is localized mainly in the tips of the young Thermomonospora vegetative hyphae.

Actinomycetales↗

PubMed2006

Yeast fluorescence microscopy.

Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells. In this chapter, detailed practical procedures for fluorescence microscopic observations developed or adopted in our laboratory are described. These include labeling of the cell wall and chitin, F-actin structures, nuclear and mitochondrial DNA, and two different procedures for investigation of yeast cells by immunofluorescence. In addition, our experience with multicolor labeling experiments is introduced and discussed.

Actins↗

PubMed2004

The absence of the Isw2p-Itc1p chromatin-remodelling complex induces mating type-specific and Flo11p-independent invasive growth of Saccharomyces cerevisiae.

The Isw2p-Itc1p chromatin remodelling complex of Saccharomyces cerevisiae is a member of the ISWI class of ATPases with a nucleosome spacing activity, involved in regulation of expression of a broad spectrum of genes. Its absence causes derepression of a-specific genes and aberrant morphology in alpha-mating type cells. We report here that the deletion of the ISW2 gene in the originally non-invasive BY strain induces mating type-specific invasive growth strongly affected by nitrogen starvation. Although the Flo11 protein was postulated to be critical for haploid invasive growth, we showed that the invasive growth caused by the isw2 and itc1 deletions in alpha-mating type cells was Flo11p-independent. This type of invasive growth was proved to be a consequence of the activation of the pheromone response pathway. Our results suggest that Isw2 and Itc1 proteins do not have the same impact on the described phenomenon.

Adenosine Triphosphatases↗