Biomedical subjects

John Y Phelps

Publications and source records attributed to John Y Phelps.

3 recordsLinked to original sources

PubMed2003

Prolactin gene expression in human ovarian follicular cells.

OBJECTIVE: To investigate prolactin gene expression in human ovarian follicular cells. DESIGN: RNA was isolated from follicular cells obtained at the time of transvaginal oocyte retrieval from patients after controlled ovarian hyperstimulation. The RNA was subjected to reverse transcription and polymerase chain reaction (RT-PCR) using prolactin-specific primers. The RT-PCR products were analyzed by gel electrophoresis, followed by Southern blot analysis using prolactin-specific probes. SETTING: Department of gynecology and obstetrics research laboratory. PATIENT(S): Women undergoing controlled ovarian hyperstimulation followed by transvaginal oocyte retrieval. INTERVENTION(S): Controlled ovarian hyperstimulation followed by transvaginal oocyte retrieval. Expression of prolactin mRNA by follicular cells. RESULT(S): The presence of prolactin-specific cDNA amplified by RT-PCR from RNA isolated from human follicular cells was confirmed by Southern blot analysis. CONCLUSION(S): Human ovarian follicular cells are an extrapituitary site of prolactin gene expression.

Blotting, Southern↗

PubMed2003

Laparoscopic suburethral sling procedure.

STUDY OBJECTIVE: To describe the technique of laparoscopic suburethral sling and discuss patient satisfaction and subjective continence rates over 7 years. DESIGN: Retrospective analysis (Canadian Task Force classification III). SETTING: Private practice. PATIENTS: Sixty-three consecutive patients. INTERVENTION: Laparoscopic sling procedure using either autologous or donor fascia. MEASUREMENTS AND MAIN RESULTS: The space of Retzius is entered laparoscopically. Then from a vaginal approach, a small midline vertical incision is made below the urethra, and vaginal epithelium is dissected off underlying periurethral and paravesical fascia. Material for the sling is passed underneath the urethra and secured laparoscopically to Cooper's ligaments bilaterally. Over 7 years, 63 laparoscopic suburethral sling procedures were performed. Overall, patients reported 88.9% satisfaction rate and 81.0% subjective continence rate. CONCLUSION: The laparoscopic approach is an acceptable alternative to other suburethral sling procedures.

Aged↗

PubMedDate not supplied

Laparoscopic vaginal vault suspension using uterosacral ligaments: a review of 133 cases.

The objective of our study was to describe the surgical technique of laparoscopic vaginal vault suspension using the uterosacral ligaments. In addition, we performed a retrospective analysis of this laparoscopic technique on 133 patients with advanced symptomatic vaginal vault prolapse to evaluate the efficacy and durability of this laparoscopic technique. Efficacy and anatomic outcome were assessed by the Baden-Walker halfway scoring system before and after the surgical procedure. Preoperatively, all patients showed evidence of grade 2 or greater prolapse (descent to the level of the hymen). Fifty-one patients (38.4%) had uterovaginal prolapse, and 82 patients (61.6%) had vaginal vault prolapse. The patients were reevaluated at 1, 6, and 12 months postoperatively and yearly thereafter. The postoperative follow-up ranged from 2.0 to 7.3 years. Postoperatively, 116 patients (87.2%) had no recurrence of prolapse, and 17 patients (12.8%) had recurrence of prolapse. The major complication rate was 2.25%. We conclude that laparoscopic vaginal vault suspension is a safe, efficacious, and durable alternative for the management of vaginal vault prolapse.

Adnexa Uteri↗