Biomedical subjects

Jong Wan Kim

Publications and source records attributed to Jong Wan Kim.

5 recordsLinked to original sources

PubMed2003

The correspondence between the labeling patterns of antibody RT97, neurofilaments, microtubule associated protein 1B and tau varies with cell types and development stages of chicken retina.

The correspondence between the labeling patterns of antibody RT97, neurofilaments (NF-M and NF-H), microtubule associated protein 1B (MAP1B) and tau, were studied in the developing chicken. At embryonic day 3 (E3), intense RT97 immunoreactivity (IR) was found to be localized in cells in the region adjacent to the intraretinal space, which separates the inner and outer layers of the optic cup, and this was sustained at E8. However, this pattern changed dramatically at E12, as the intensities of RT97 IR increased in the inner retinal layer, while the outermost layers showed only weak IR. The adult stage retina showed RT97 IR within the nerve fibers of the ganglion cells, the processes of the amacrine cells and the photoreceptors. Additional immunostainings for NF-M, -H, MAP1B and tau showed that the observed changes in RT97 IRs were due to the different expressions of these proteins at different development stages.

Animals↗

PubMed2003

Caveolin-3 expression during early chicken development.

Caveolin-3, a protein that is correlated with caveolae, is found in muscle cells, especially during their differentiation. Although the distribution of caveolin-3 has been studied in cases such as adult and late embryonic mammalians, the expression of caveolin-3 has not been clearly defined during chicken development. In this study, we detected intense caveolin-3 immunoreactivity (IR) as early as embryonic day 4 (E4), most of the signals were localized within the neural tube and myotome. While IRs in the brain occurred in radial glia at E6, these intensities were reduced to an almost undetectable level at E8. In the case of muscle cells, the exclusive localization of caveolin-3 in the cytoplasmic membrane was detected even at E11, much earlier than in mammalian muscle tissues. Although the caveolin-3 IR pattern was similar to that reported by previous studies, we found some interesting mismatches in the case of avian tissues. Although we are unable to explain caveolin-3 expression patterns in the early embryonic stages, this study could provide a basis for further study on the function of caveolin-3 in avian embryogenesis.

Animals↗

PubMed2003

Ultramicroscopical immunolocalization of PAX6 in the adult chicken retina.

Cell type-specific PAX6 protein expression was examined in all retinal layers of the normal chicken retina. The most intense PAX6 immunostaining was found in the ganglion cell and inner nuclear layers, and in lower amounts in the optic nerve fiber, the inner plexiform and the photoreceptor layers. PAX6 immunostaining was variable in terms of its subcellular localization, even within one cell. PAX6 immunostaining was mainly localized in nuclear heterochromatin of the ganglion cell and inner nuclear layers whereas in the outer nuclear layer, PAX6 immunostaining was only observed in the intercellular space and the cytoplasm. In photoreceptors, the myoid portion of the inner segment showed PAX6 immunostaining, but the ellipsoid portion and the outer segment did not. The ultrastructural distribution pattern of PAX6 in the adult chicken retina suggests that normal expression of PAX6 is variable even in subcellular structures in the same cell type.

Animals↗

PubMed2002

Molecular analysis of the accumulation of the transcripts of the large subunit gene of ribulose-1,5-bisphosphate carboxylase/oxygenase by light.

We previously reported that the rbcL transcript level was significantly increased by light signal [Lee and Sim (1995)]. To investigate whether or not the accumulation of rbcL mRNA by light signal is caused by an increase of transcription activity of the rbcL gene, runon transcription assays were performed The results indicated that the accumulation of rbcL mRNA is due to an increase in the transcriptional activity of the rbcL gene by light. An electrophoretic mobility shift assay (EMSA) was also carried out to elucidate the specific binding proteins that interact with the rbcL promoter region. As a result of EMSA, a plastid protein designated as the rbcL promoter binding (RLPB) protein factor was detected in the plastid extract of light-grown seedlings, but not in that of dark-adapted seedlings before harvesting. It was also ascertained in this study that the promoter core region for the RLPB protein factor to bind is between -3 and -32 nucleotide sequences from the transcription initiation site of the rbcL gene. These results suggest that the stimulation of rbcL transcription by light is, in part, due to the increase in binding of the RLPB protein factor to the rbcL promoter.

DNA-Binding Proteins↗

PubMed2002

The clinical features of Behcet's disease in Yongdong districts: analysis of a cohort followed from 1997 to 2001.

This study was undertaken to evaluate the clinical features of a cohort of Behcet's disease (BD) followed up from January 1997 to July 2001 in Yongdong districts in Korea, and to compare the results with the literature. Overall features of clinical manifestations were similar to those described in the literature. However, the frequency of gastrointestinal (GI) ulcerations was much higher than those of other Korean studies. The Korean studies including ours revealed a lower frequency of vascular lesions and epididymitis compared with studies of other countries. The most common site and pattern of inflammatory arthritis were knees and monoarticular involvement, respectively. In addition, in most patients, the ocular lesions involved the posterior uveal tract, and the terminal ileum and cecum were the most common sites of GI involvement. Patients with ocular lesions or GI lesions showed a good prognosis during the follow-up. The HLA-B51 antigen was positive in 50.7% of patients, and it was more commonly found in patients with a familial BD.

Adult↗