Biomedical subjects

Jong-Bok Kim

Publications and source records attributed to Jong-Bok Kim.

8 recordsLinked to original sources

PubMed2005

Dynamic pH junction technique for on-line preconcentration of peptides in capillary electrophoresis.

A method based on the presence of a dynamic pH junction within the capillary to induce band narrowing for enhanced detection sensitivity for some peptides is presented. This technique is predicated on a sharp reduction in an analyte's migration velocity following a reversal of its electrophoretic direction from the acidic sample zone to the basic BGS zone. Larger-than-usual injection volumes of samples in relatively high-conductivity matrices were enabled, without degrading peak shape, resolution and efficiency. The size of the original sample plug was reduced by as much as 38-fold, and improvement in detector response in terms of peak height by as much as 124-fold was obtained. The effects of pH and concentration of the sample matrix, and the length of sample injection on the efficiency of the technique are discussed.

Angiotensin I↗

PubMed2003

On-line sample preconcentration of cationic analytes by dynamic pH junction in capillary electrophoresis.

To improve detection sensitivity of cationic analytes, a dynamic pH junction technique was examined. Dynamic pH junction is an on-line focusing method in capillary electrophoresis (CE) based on the difference in the analyte's mobility between the background electrolyte (BGE) and sample matrix. The effects of pH values and concentrations of the BGE and the sample matrix on dynamic pH junction were examined. Optimization of analyte focusing resulted in enhanced detection responses of about 100-160-fold in terms of peak heights for some anilines in comparison to conventional injections. In particular, the concentration limits of detection (LOD) (S/N = 3) for the test anilines obtained with dynamic pH junction were from 1.9 to 3.7 ppb with UV detection without any pretreatment procedure.

Cations↗

PubMed2003

Mechanistic study on analyte focusing by dynamic pH junction in capillary electrophoresis using computer simulation.

Dynamic pH junction is an on-line preconcentration method in capillary electrophoresis (CE) based on electrokinetic focusing of weakly ionic analytes with in large sample volumes in a multisection electrolyte system. In this report, experiments and computer simulations were performed to gain a better insight of the analyte focusing mechanism when a dynamic pH junction was used. A computer program, SIMUL, was used to simulate the band-narrowing process of a group for phenol derivatives under optimized buffer conditions, which were compared with experimental results. Computer simulations revealed the formation of a sharp moving pH boundary within the sample zone causing efficient focusing of long plugs of weakly acidic analytes based on their pKa. These studies offered useful information for understanding the band-narrowing process by control of the depth and lifetime of the moving pH boundary as a function of analyte pKa, sample pH, and injection length. The change in pH of the sample within the capillary was also estimated by measuring the absorbances of an analyte at two different wave-lengths. Optimization of analyte focusing resulted in enhanced detection responses of about 60-450-fold in terms of peak heights for some phenol derivatives' relation to conventional injections. Dynamic pH junction represents a novel approach to control band dispersion (peak width) and selectivity (mobility) of specific analytes for high-resolution CE separations.

Journal Article↗

PubMed2003

On-line sample preconcentration techniques in micellar electrokinetic chromatography.

This review provides an overview as well as a practical understanding of on-line sample concentration techniques in micellar electrokinetic chromatography (MEKC). MEKC as well as other capillary electrophoretic modes suffer from low concentration sensitivity due to minute sample volume and limited optical pathlength for on-capillary photometric detection. Two on-line sample preconcentration techniques, sample stacking and sweeping are known to be effective techniques for enhancement of the concentration sensitivity in MEKC. Sample stacking occurs as ions cross a boundary that separates regions of the high electric field sample zone and the low electric field background solution zone. The difference in migration velocity of pseudostationary phases within the two zones is the key to achieving the focusing effect. Sweeping is defined as the picking and accumulating of analytes by the pseudostationary phase that penetrates the sample zone devoid of pseudostationary phase. In this review, several examples of the sample stacking and sweeping under different experimental conditions are given, besides many references to applications.

Chromatography, Micellar Electrokinetic Capillary↗

PubMed2003

On-line preconcentration and enantioselective separation of triadimenol by electrokinetic chromatography using cyclodextrins as chiral selectors.

Enantioselective separation of triadimenol, a component of systemic agricultural fungicide, by electrokinetic chromatography (EKC) using cyclodextrins (CDs) as chiral selectors was investigated. Both a neutral CD derivative, hydroxypropyl-gamma-CD (HP-gamma-CD), and an ionic one, heptakis-6-sulfato-beta-CD (HS-beta-CD), were employed as an additive in cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) and as a chiral pseudostationary phase in CDEKC, respectively. In each system, four stereoisomeric peaks were completely or partially separated from each other. To enhance the detectability or the concentration sensitivity, on-line preconcentration techniques were applied to both EKC systems. Sweeping was used in the CD-MEKC system under an acidic condition, whereas stacking with a reverse migrating pseudostationary phase (SRMP) in the CDEKC system. Around 10-fold increase in the detection sensitivity for each peak was attained with both sweeping and SRMP systems. Good repeatabilities in the migration time, corrected peak area, and peak height were recognized in terms of the relative standard deviation. The limit of detection for each peak in the SRMP-CDEKC system, calculated from the calibration curve, was found to be 0.8-3.8 ppm.

Chromatography, Micellar Electrokinetic Capillary↗

PubMed2002

On-line sample preconcentration in micellar electrokinetic chromatography using ion-pair reagents.

To improve the detection sensitivity of some aromatic carboxylic acids and naphthalenesulfonic acids, the use of ion-pair reagents was examined in sweeping micellar electrokinetic chromatography (MEKC) with an anionic sodium dodecyl sulfate (SDS) micelle. Tetraalkylammonium (TAA) salts were used as ion-pair reagents to improve the sweeping efficiency. The effects of the alkyl chain length of the TAA groups and the TAA salt concentration on sweeping were examined. Under optimized conditions, about 400-fold enhancement in detection sensitivity was obtained in terms of peak heights by addition of ion-pair reagents in sweeping MEKC. This value was about 10 times greater than that obtainable by the SDS micelle used alone.

Carboxylic Acids↗

PubMed2002

Sweeping: concentration mechanism and applications to high-sensitivity analysis in capillary electrophoresis.

Sweeping in capillary electrophoresis (CE) involves the interaction of a pseudostationary phase (PS) in the separation solution and a sample in the matrix that is free of the PS used. The PS includes not only the PSs employed in electrokinetic chromatography, but also complexation reagents such as borate. The sample matrix could have a lower, similar, or higher conductance than the separation solution. Thus, the basic condition for sweeping is a sample matrix free of the additive. The accumulation of analyte molecules during the interaction makes this interesting phenomenon very useful as an on-line preconcentration method for CE. Preconcentration occurs due to chromatographic partitioning, complexation, or any interaction between analytes and PS. Contact between analyte and PS is facilitated by the action of electrophoresis and is independent of electroosmosis. The analyte, PS, or both should have electrophoretic velocities when an electric field is applied. The extent of preconcentration is dictated by the strength of the interaction involved. From tens to several thousand-fold improvements in detector response for many neutral and charged analytes have been achieved with this technique, suggesting sweeping as a general approach to on-line preconcentration in CE. The mechanism and applications of the sweeping phenomenon under different experimental conditions are discussed in this review, with particular emphasis on a better understanding of the sweeping mechanism under reduced electric field (high conductivity) in the sample zone.

Electrophoresis, Capillary↗

PubMed2002

Analysis of the herbicides paraquat, diquat and difenzoquat in drinking water by micellar electrokinetic chromatography using sweeping and cation selective exhaustive injection.

Optimum conditions for the determination of the herbicides paraquat, diquat and difenzoquat by micellar electrokinetic chromatography (MEKC) using sweeping and cation-selective exhaustive injection (CSEI) as on-line concentration methods were developed. Sodium dodecyl sulfate (80 mM) in 50 mM phosphate buffer (pH 2.5) with 20% acetonitrile was used as a background electrolyte for the methods studied. The limits of detection, based on a signal-to-noise ratio of 3:1, were about 2.6-5.1 mg 1(-1) in purified water when MEKC was applied for the standards. By using an on-line preconcentration method known as sweeping-MEKC, up to a 500-fold increase in detection sensitivity was obtained whereas up to a 50 000-fold increase for CSEI-sweeping-MEKC was achieved. The limits of detection using optimum CSEI-sweeping-MEKC were lower than 1 microg 1(-1) and the method was validated obtaining good reproducibility (relative standard deviation lower than 22%) and linearity. CSEI-sweeping-MEKC was successfully applied to the determination of the three herbicides in spiked tap water below the levels established by the US Environmental Protection Agency.

Cations↗