Biomedical subjects

Joseph Mizrahi

Publications and source records attributed to Joseph Mizrahi.

9 recordsLinked to original sources

PubMed2006

Immobilized fibrinogen in PEG hydrogels does not improve chondrocyte-mediated matrix deposition in response to mechanical stimulation.

The present investigation aims to explore the role of cell-scaffold interactions and whole cell compression in chondrocyte mechanotransduction using encapsulating poly(ethylene glycol) (PEG) hydrogel scaffolds and primary bovine chondrocytes. Scaffolds made from PEG hydrogels with immobilized fibrinogen molecules were seeded with chondrocytes and subjected to 15% dynamic compressive strain at 1-Hz frequency. Dynamic strain stimulation resulted in a 37% increase in the levels of sulfated glycosaminoglycan (sGAG) after 2 weeks of stimulation, when compared to static controls. Comparing results of the PEG-fibrinogen scaffolds with their respective PEG control group did not show significant differences between the two, even following 2 weeks of dynamic mechanical stimulation. Accordingly, these findings indicate that while cell deformations cause metabolic changes in chondrocytes seeded in PEG hydrogels, it is difficult to ascertain the role of matrix bioactivity in enhancing chondrocyte mechanotransduction in encapsulating scaffolds subjected to physical deformations. This study shows how interactions between mechanical stimulation and scaffold composition are evaluated using an experimental approach and customized biomaterial scaffolds.

Animals↗

PubMed2006

Evaluation of methods for extraction of the volitional EMG in dynamic hybrid muscle activation.

BACKGROUND: Hybrid muscle activation is a modality used for muscle force enhancement, in which muscle contraction is generated from two different excitation sources: volitional and external, by means of electrical stimulation (ES). Under hybrid activation, the overall EMG signal is the combination of the volitional and ES-induced components. In this study, we developed a computational scheme to extract the volitional EMG envelope from the overall dynamic EMG signal, to serve as an input signal for control purposes, and for evaluation of muscle forces. METHODS: A "synthetic" database was created from in-vivo experiments on the Tibialis Anterior of the right foot to emulate hybrid EMG signals, including the volitional and induced components. The database was used to evaluate the results obtained from six signal processing schemes, including seven different modules for filtration, rectification and ES component removal. The schemes differed from each other by their module combinations, as follows: blocking window only, comb filter only, blocking window and comb filter, blocking window and peak envelope, comb filter and peak envelope and, finally, blocking window, comb filter and peak envelope. RESULTS AND CONCLUSION: The results showed that the scheme including all the modules led to an excellent approximation of the volitional EMG envelope, as extracted from the hybrid signal, and underlined the importance of the artifact blocking window module in the process. The results of this work have direct implications on the development of hybrid muscle activation rehabilitation systems for the enhancement of weakened muscles.

Journal Article↗

PubMed2006

Objective measurement of knee extension force based on computer adaptive testing.

False impairment is encountered when tested subjects either unintentionally or deliberately put an artificial upper limit on their force, in which case their true capacity cannot be disclosed in a straight forward measurement. The aim of this study was to develop a computer adaptive testing (CAT) system for directing subjects into generating greater forces than they intended. The system was tested on eleven cooperative female subjects who volunteered to take part in this study. The CAT consisted of interactive testing cycles, each containing a series of isometric tasks of differing intensities. While fulfilling these tasks, the tested subjects were asked to take care not to exceed a self-selected upper force limit (F(ssl)) that they were previously trained to memorize (order of 40% of the maximal voluntary contraction). Visual feedback, displaying the applied force exertions, was provided to the tested subjects but was modified by re-scaling the display in an un-anticipated manner. To confirm the subject's ability to remember her F(ssl), repeatability of joint memory was tested one week after the CAT. The CAT results were successful in causing ten out of the eleven tested participants to exert a higher force than they intended to. Additionally, the CAT algorithm caused a statistically significant higher force than the repeatability test. These results demonstrate the potential of CAT methods in improving the clinical evaluation of muscle strength, particularly in those cases where the subject's cooperation is not sufficient.

Adult↗

PubMed2006

Partition between volitional and induced forces in electrically augmented dynamic isometric muscle contractions.

Augmentation of force in partially deficient muscles can be achieved by combining electrical stimulation (ES) with their volitional activation (hybrid activation). However, while the overall torque results from the combination of the volitional and the electrically-induced torque components, the exact share between these components is not known. In a previous work, we described a method to resolve the share between the torque components under isometric static contractions. In this work, we extend our analysis to the case of isometric dynamic contractions. Five healthy subjects were instructed to contract their Tibialis Anterior (TA) muscles according to a typical gait-like dynamic torque pattern, that was visually displayed to them, while monitoring their actual ankle torque and TA electromyography (EMG). These experiments were done with and without augmented activation by means of ES. A computational algorithm was developed to dissociate the volitional from the overall torque, based on EMG signal processing and on precalibration of the dynamic system of the volitional torque versus EMG. The results indicated the quantitative relations between decrease in the volitional torque and the required increase in ES enhancement. The developed method also demonstrated what ES intensity profile is necessary to produce a desired overall torque output. This provides the means for designing an adaptive rehabilitation device for the hybrid activation of deficient muscles.

Adult↗

PubMed2005

Generalized cable equation model for myelinated nerve fiber.

Herein, the well-known cable equation for nonmyelinated axon model is extended analytically for myelinated axon formulation. The myelinated membrane conductivity is represented via the Fourier series expansion. The classical cable equation is thereby modified into a linear second order ordinary differential equation with periodic coefficients, known as Hill's equation. The general internal source response, expressed via repeated convolutions, uniformly converges provided that the entire periodic membrane is passive. The solution can be interpreted as an extended source response in an equivalent nonmyelinated axon (i.e., the response is governed by the classical cable equation). The extended source consists of the original source and a novel activation function, replacing the periodic membrane in the myelinated axon model. Hill's equation is explicitly integrated for the specific choice of piecewise constant membrane conductivity profile, thereby resulting in an explicit closed form expression for the transmembrane potential in terms of trigonometric functions. The Floquet's modes are recognized as the nerve fiber activation modes, which are conventionally associated with the nonlinear Hodgkin-Huxley formulation. They can also be incorporated in our linear model, provided that the periodic membrane point-wise passivity constraint is properly modified. Indeed, the modified condition, enforcing the periodic membrane passivity constraint on the average conductivity only leads, for the first time, to the inclusion of the nerve fiber activation modes in our novel model. The validity of the generalized transmission-line and cable equation models for a myelinated nerve fiber, is verified herein through a rigorous Green's function formulation and numerical simulations for transmembrane potential induced in three-dimensional myelinated cylindrical cell. It is shown that the dominant pole contribution of the exact modal expansion is the transmembrane potential solution of our generalized model.

Action Potentials↗

PubMed2003

Joint torques during sit-to-stand in healthy subjects and people with Parkinson's disease.

OBJECTIVES: To compare lower limb joint torques during sit-to-stand in normal elderly subjects and people with Parkinson's disease, using a developed biomechanical model simulating all phases of sit-to-stand.Design. A cross-sectional study utilizing a Parkinsonian and a control group. BACKGROUND: Subjects with Parkinson's disease were observed to experience difficulty in performing sit-to-stand. The developed model was used to calculate the lower limb joint torques in normal elderly subjects and subjects with Parkinson's disease, to delineate possible causes underlying difficulties in initiating sit-to-stand task. METHODS: Six normal elderly subjects and seven age-matched subjects with Parkinson's disease performed five sit-to-stand trials at their self-selected speed. Anthropometric data, two-dimensional kinematic and foot-ground and thigh-chair reactive forces were used to calculate, via inverse dynamics, the joint torques during sit-to-stand in both before and after seat-off phases. The difference between the control and Parkinson's disease group was analysed using independent t-tests. RESULTS: Both control and Parkinson's disease groups had a similar joint kinematic pattern, although the Parkinson's disease group demonstrated a slower angular displacement. The latter subjects produced significantly smaller normalized hip flexion torque and presented a slower torque build-up rate than the able-bodied subjects (P<0.05). CONCLUSION: Slowness of sit-to-stand in people with Parkinson's disease could be due to a reduced hip flexion joint torque and a prolonged rate of torque production.

Ankle Joint↗

PubMed2003

Constant and variable stiffness and damping of the leg joints in human hopping.

The present study deals with the stiffness and damping profiles of the leg joints during the ground-contact phase of hopping. A two-dimensional (sagittal plane) jumping model, consisting of four linked rigid segments and including the paired feet, shanks, thighs, and the head-arms-trunk segment, was developed. The segments were interconnected by damped torsional springs, representing the action of the muscles, tendons and ligaments across the joint and of the other joint tissues. A regressive function was used to express stiffness and damping, and included second-order dependence on angle and first-order dependence on angular velocity. By eliminating redundancies in the numerical solution using multicollinearity diagnostic algorithms, the model results revealed that the correct and sufficient nonlinearity for the joint stiffness is of the first order. Damping was found negligible. The stiffness profiles obtained were bell-shaped with a maximum near midstance and nonzero edge values. In predicting the joint moments, the obtained variable joint stiffnesses provided a closer agreement compared to a constant stiffness model. The maximal stiffness was found to be in linear correlation with the initial stiffness in each joint, providing support to the of muscles' preactivation strategy during the flight phase of hopping. All stiffnesses increased with increasing hopping frequency. The model presented provides an effective tool for future designing of artificial legs and robots and for the development of more accurate control strategies.

Adult↗

PubMed2002

Rigorous Green's function formulation for transmembrane potential induced along a 3-D infinite cylindrical cell.

The quasi-static electromagnetic field interaction with three-dimensional infinite-cylindrical cell is investigated for both intracellular (IPS) and extracellular (EPS) current point-source excitation. The induced transmembrane potential (TMP), expressed conventionally via Green's function, may alternatively be expanded into a faster-converging representation using a complex contour integration, consisting of an infinite-discrete set of exponentially decaying oscillating modes (corresponding to complex eigenvalues) and a continuous source-mode convolution integral. The dominant contributions for both the IPS and EPS problems are obtained in simple closed-form expressions, including well documented special mathematical functions. In the IPS case, the dominant modal contribution (of order zero)--an exact solution of the well-known cable equation--is explicitly and analytically corrected by the imaginary part of its eigenvalue and the source-mode convolution contribution. However, the TMP along a fiber was shown to decay at infinity algebraically and not exponentially, as predicted by the classic cable equation solution. In the EPS case, the dominant contribution is expressed as a source-mode convolution integral. However, for a long EPS distance (e.g., >10 cable length constant) the order-one-modes involved in the convolution is not a solution of the cable equation. Only for shorter EPS distance should the cable equation solution (i.e., the order zero dominant mode) be included in addition to the modes of order one. For on-membrane EPS location, additional modes should be included as well. In view of our EPS result, we suggest that the cable equation modeling existing in the literature and related to functional electrical stimulation for EPS problems, should be critically reviewed and corrected.

Axons↗

PubMed1998

Dynamic loading on the human musculoskeletal system -- effect of fatigue.

OBJECTIVE: A study was conducted to investigate the effects of fatigue on the ability of human musculoskeletal system to deal with the onslaught of the heel strike initiated shock waves. DESIGN: Running on a treadmill at the anaerobic threshold level for 30 min was used to acquire the experimental data on the foot strike initiated shock waves. BACKGROUND: Muscles act to lower the bending stress on bone and to attenuate the dynamic load on human musculoskeletal system. Fatigue may diminish their ability to dissipate and attenuate loading on the system. Knowledge of the effects of fatigue on the ability of the human musculoskeletal system to attenuate the shock waves may help in design of the training procedures and exercises. METHODS: Twenty-two young healthy males participated in this study. Each one was running on the treadmill at the speed corresponding to his anaerobic threshold for 30 min. The heel strike induced shock waves were recorded every 5 min on the tibial tuberosity and sacrum. The data obtained were analyzed in both temporal and frequency domains. RESULTS: The results reveal significant increase in the dynamic loading experienced by the human musculoskeletal system with fatigue. This may be attributed to the inability of the fatigued system to provide an efficient way to attenuate shock waves. CONCLUSIONS: The analysis of the recorded signals suggests that fatigue contributes to the reduction of the human musculoskeletal system's capacity to attenuate and dissipate those shock waves. This capacity appears to be a function not only of the fatigue level, but also of the vertical location along the skeleton. RELEVANCE: Fatigue during running may affect the ability of the human musculoskeletal system to attenuate and dissipate the heel strike induced shock waves. The study of the fatigue effect on shock wave attenuation provides information that may benefit the runner.

Journal Article↗