Biomedical subjects

Juan Bautista

Publications and source records attributed to Juan Bautista.

5 recordsLinked to original sources

PubMed2006

Lipophilic hydroxytyrosyl esters. Antioxidant activity in lipid matrices and biological systems.

Antioxidant activities of lipophilic hydroxytyrosyl acetate, palmitate, oleate, and linoleate were compared with those of hydroxytyrosol, alpha-tocopherol, and butylhydroxytoluene (BHT) in both glyceridic matrix and biological systems. Aliquots of a glyceridic matrix spiked with various concentrations of antioxidant were subjected to accelerated oxidation in a Rancimat apparatus operated at 90 degrees C. The relationships between induction time (IT) and antioxidant concentration (mmol/kg) presented by hydroxytyrosol and hydroxytyrosyl acetate, palmitate, oleate, and linoleate were similar. Hydroxytyrosol and its esters showed greater antioxidant activity than alpha-tocopherol or BHT. We also evaluated the capacity of hydroxytyrosyl esters to protect proteins and lipids against oxidation caused by peroxyl radicals, using a brain homogenate as an ex vivo model. All tested compounds showed a protective effect in these systems, which was greater in preventing the generation of carbonyl groups in protein than of malondialdehyde in lipid. Inclusion of a lipophilic chain in the hydroxytyrosol molecule enhanced its antioxidant capacities in this biological model.

Acetates↗

PubMed2006

Association of novel POLG mutations and multiple mitochondrial DNA deletions with variable clinical phenotypes in a Spanish population.

BACKGROUND: Both dominant and recessive mutations were reported in the gene encoding the mitochondrial (mt) DNA polymerase gamma (POLG) in patients with progressive external ophthalmoplegia (PEO). Phenotypes other than PEO were recently documented in patients with mutations in the POLG gene. OBJECTIVE: To screen patients with mitochondrial disease and multiple mtDNA deletions in muscle for mutations in the coding regions of the POLG, PEO1, and SLC25A4 genes. DESIGN: To identify the underlying molecular defect in a group of patients with multiple mtDNA deletions comparing their molecular genetic findings with those of healthy controls. PATIENTS: Twenty-four patients (16 men and 8 women) diagnosed with mitochondrial disease and having multiple mtDNA deletions in muscle by Southern blot analysis. Thirteen patients had PEO; 2 had PEO alone, 4 had PEO and myopathy, and 5 had PEO and multisystem involvement. Four patients had multisystem disease without PEO. The remaining 9 patients had isolated myopathy. DNA from 100 healthy individuals was also studied. RESULTS: No mutation was identified in the PEO1 or SLC25A4 genes. Nine POLG mutations were observed in 6 of 24 patients. Four novel mutations were detected and mapped in the linker region (M603L) and in the pol domain of the enzyme (R853W; D1184N; R1146C). Five patients with PEO had mutations: 2 were compound heterozygotes, 1 was homozygous, and another showed a mutation in a single allele. The remaining patient also showed a sole mutation and had an unusual phenotype lacking ocular involvement. CONCLUSIONS: POLG molecular defects were found in 25% of our patients with multiple mtDNA deletions and mitochondrial disease. The uncommon phenotype found in 1 of these patients stresses the clinical variability of patients harboring POLG mutations. Molecular studies in the POLG gene should be addressed in patients with mitochondrial disease, particularly in those with PEO, and multiple mtDNA deletions.

Adenine Nucleotide Translocator 1↗

PubMed2005

Exercise training in mitochondrial myopathy: a randomized controlled trial.

Patients with mitochondrial myopathies (MM) usually suffer from exercise intolerance due to their impaired oxidative capacity and physical deconditioning. We evaluated the effects of a 12-week supervised randomized rehabilitation program involving endurance training in patients with MM. Twenty MM patients were assigned to a training or control group. For three nonconsecutive days each week, patients combined cycle exercise at 70% of their peak work rate with three upper-body weight-lifting exercises performed at 50% of maximum capacity. Training increased maximal oxygen uptake (28.5%), work output (15.5%), and minute ventilation (40%), endurance performance (62%), walking distance in shuttle walking test (+95 m), and peripheral muscle strength (32%-62%), and improved Nottingham Health Profile scores (21.47%) and clinical symptoms. Control MM patients did not change from baseline. Results show that our exercise program is an adequate training strategy for patients with mitochondrial myopathy.

Adolescent↗

PubMed2003

Prevention of brain protein and lipid oxidation elicited by a water-soluble oryzanol enzymatic extract derived from rice bran.

BACKGROUND: The antioxidant capacity of rice bran (RB) (due mainly to its gamma-oryzanol content) is very well known. We have recently developed a water-soluble oryzanol enzymatic extract (WSOEE), which shows a greatly increased functionality. AIM OF THE STUDY: The aim of our study is the evaluation of the antioxidant potential of WSOEE in an ex vivo model to compare its protective capacity against oxidative damage by active-oxygen substances and free radicals (mainly the peroxyl radical) to biomolecules (such as proteins and lipids) with that of antioxidants, such as Trolox (a water-soluble derivative of vitamin E), melatonin, and folic acid. METHODS: WSOEE gamma-oryzanol content and composition were determined by HPLC. Free-radical-scavenging capacity was evaluated using the assay based on phycoerythrin fluorescence. Antioxidant capacity against hydroperoxide-caused oxidative injury to proteins and lipids was evaluated using an ex vivo model: a rat brain homogenate. The effectiveness was determined by assessing protein damage (measured as carbonyl group content by Western blot immunoassay) and lipid peroxidation (measured as malondialdehyde content). RESULTS: The WSOEE gamma-oryzanol composition profile was similar to that of RB (cycloartenyl, 24-methylene cycloartenyl, campesteryl, and sitosteryl ferulates), but with two major differences: WSOEE gamma-oryzanol concentration was five times higher than that of RB, and WSOEE was water soluble. WSOEE total antioxidant capacity to trap the peroxyl radical was high, and similar to that of Trolox. The capacity to inhibit lipid peroxidation induced by cumene hydroperoxide in rat brain homogenate yielded a protection similar to that of Trolox. WSOEE also showed the capacity to protect protein from oxidation phenomena in rat brain homogenate, with a behavior similar to that of melatonin. This is of particular importance, since the loss of protein function caused by oxidative modification may affect the activity of enzymes, receptors, and membrane transporters, among other functions. CONCLUSION: WSOEE is a new potential antioxidant agent from rice bran that shows a high free-radical-scavenging capacity and prevents protein oxidation and lipid peroxidation when cells ex vivo are exposed to free radicals.

Animals↗

PubMedDate not supplied

[CHOLEDOCHAL CYST: ENDOSCOPIC RETROGRADE CHOLANGIOPANCREATOGRAPHY(ERCP) DIAGNOSIS VALUE]

We presented six cases of choledochal cysts all of whichwere diagnosed by ERCP. Patients were 12,13,17,20,29 and 36 years old. Five were female and one was a male. The main symptom was abdominal pain.The Endoscopic Retrograde Cholangiopancreatography showed the anatomic details of the biliary tree and the pancreaticobiliary ductal junction and also allowed us to decide the adecuate management of the patient.

Journal Article↗