Biomedical subjects

Julian Alonso

Publications and source records attributed to Julian Alonso.

3 recordsLinked to original sources

PubMed2007

New hexamethine-hemicyanine dyes for the development of integrated optochemical sensors.

New far-visible absorbing anilino-cyanine dyes have been synthesised for future application as chromoionophores in integrated waveguide absorbance optodes based on bulk optodes. The effect of the heterocycle, of the substitution of the heterocyclic nitrogen and of the type of heptamethine central ring on the pKa values (4.3-8.2 in ethanol-water solutions and 9.5-11.0 in plasticised PVC membranes), on the spectroscopic characteristics of the dye and on photostability is discussed. pH-selective bulk optodes have been formulated as a first approach to develop ion-selective optodes, and sensitivity, repeatability, lifetime and response time have been determined. The dyes show good analytical behaviour for use as chromoionophores for the development of ion-selective optodes. Reversible (80-87%), fast (tr90%=0.94-2.28 min) and pH-sensitive membranes (slopes of 0.09-0.23 DeltaAbs.pHdec-1, absorbance range 0.19-0.53) have been obtained. Moreover, they exhibit good spectroscopic features for employment with integrated optochemical sensors: absorption maxima of the acidic species in plasticised PVC membranes matched those of 650-670-nm LEDs, high molar absorption coefficients (epsilonacidic=3.5x10(4)-9.3x10(4) L mol-1 cm-1 and epsilonbasic=1.9x10(4)-6.7x10(4) L mol-1 cm-1) and fluorescence.

Aniline Compounds↗

PubMed2004

In-soil potassium sensor system.

A potentiometric sensor system based on potassium ion-selective electrodes was developed for agricultural purposes. Sensors were built using PVC ion-selective membranes over an inner solid contact prepared with graphite-epoxy composites. A copper plate was used as a reference electrode. A two-stage electronic circuit composed of current and voltage amplifiers was designed to interface the sensors to a distributed data acquisition system. Three ion-selective sensors and three off-the-shelf temperature sensors and their associated circuits were mounted in a PVC tube to set up a soil probe. The electronic controls were placed in an airtight box fixed at the upper part of the probe. The system was evaluated in the field, where the sensors presented sensibility within the range of 69-71 mV dec(-)(1). Extracts of soil samples were analyzed by a current flame photometry approach, and the results, compared with the probe measurements, showed a linear relationship (r (2) = 0.992 and 0.995, respectively, to 5 and 20 cm depths), which implies viability and instrumentation reliability for agricultural applications.

Ion-Selective Electrodes↗

PubMed2002

Ketocyanine dyes: H+ selective lonophores for use in integrated waveguides absorbance optodes.

The optical and analytical characteristics of a series of neutral H+-selective chromoionophores in PVC membranes are described. Such indicators have been synthesized so that they can operate in bulk optode membranes as the chemically active region of integrated waveguide absorbance optodes (IWAOs), lambdamax near 780 nm. Their spectral characteristics, acid-base properties, chemical stability, and solubility in the membrane phase are given and discussed. The response characteristics are first tested in a conventional absorbance/transmittance flow cell. They offer a wide range of pKa's in PVC membranes, good sensitivity as a result of their high molar absortivities, excellent solubility in the plasticizer, and quick response times. They present good chemical stability in common laboratory conditions when stored in the dark, and the absence of leaching guarantees a long lifetime. Membranes have been finally applied as the sensing region of an integrated waveguide optode, demonstrating the extraordinary sensitivity improvement while preserving the remaining analytical features. Calibration curve slopes are multiplied by 3-28, and response times lower than 2 min are obtained.

Journal Article↗