Biomedical subjects

Jun Yang

Publications and source records attributed to Jun Yang.

At least 199 records · Page 11Linked to original sources

PubMed2005

[A comparative study of E-cadherin mRNA expression in primary tumors and metastatic foci of gastric cancer].

OBJECTIVE: To evaluate the expression level of E-cadherin (E-CD) mRNA in gastric cancer and its relation to biological behavior. METHODS: The expression level of E-CD mRNA was examined by RT-PCR in primary tumors, nearby tumor free tissues, metastatic lymph nodes and peritoneal lavage fluid from 35 cases with advanced gastric cancer. RESULTS: All the specimens expressed E-CD mRNA. The expression level in primary tumors was significantly decreased compared with that in nearby tumor free tissues (P < 0.01). The decrease in E-CD expression was more marked in poorly differentiated, diffusely growing tumors invading through serosal membrane. E-CD expression in the primary tumor was more significantly decreased with the increase in the number of lymph nodes (LNS) metastasized and progression of the clinical stage. However, E-CD expression level was significantly increased in metastatic LNs. The difference in E-CD mRNA level between metastatic LN and the primary tumor was increasing with progression of the disease. The expression level of E-CD mRNA in peritoneal lavage fluid was increased in patients whose tumor had penetrated through serosa and larvage fluid was positive on exfoliative cytologic examination. CONCLUSION: E-CD mRNA expression in primary tumors of gastric cancer is negatively correlated with the malignant behavior of the tumor. Nonetheless, that in the metastatic lymph nodes is increased with the progression of the disease.

Adult↗

PubMed2005

[Low-dose gemcitabine promotes radiation-induced apoptosis of human hepatoma cell line SMMC-7721].

OBJECTIVE: To observe the effect and explore the mechanism of low-dose gemcitabine (GEM) in promoting the apoptosis of human hepatoma cell line SMMC-7721 induced by radiation. METHODS: The SMMC-7721 cells were divided into four groups to serve as the control group or exposed to treatment with radiation only, GEM only, and both GEM and radiation, respectively. MTT assay was used to evaluate the activity of SMMC-7721 cells at different conditions, and the apoptosis rate and morphologic changes of the cells after treatment were observed under optical microscope, transmission electron microscope and fluorescence microsope, respectively. RESULTS: Treatment with either radiation or GEM alone was capable of inducing cell apoptosis, and GEM significantly increased the cell apoptosis induced by radiotherapy. CONCLUSION: Low-dose GEM can significantly enhance radiation-induced apoptosis of SMMC-7721 cells.

Apoptosis↗

PubMed2005

[The effect of early fluvastatin therapy on inflammatory factors in acute coronary syndrome].

OBJECTIVE: To investigate the effects of different doses of fluvastatin on serum levels of high-sensitive C-reactive protein (hs-CRP) and tumour necrosis factor-alpha (TNFalpha) in the early phase of acute coronary syndrome (ACS). METHODS: Fifty eight patients with ACS were randomly separated into three groups. 20 patients in group A were given routine therapy, 20 patients in group B were given routine therapy with oral fluvastatin 40 mg once daily for 1 week and 18 patients in group C received routine therapy with oral fluvastatin 80 mg once daily for 1 week. 20 patients with stable coronary heart disease served controls. The serum levels of hs-CRP and TNFalpha before and after therapy were measured with immunoturbidimetric assay and ELISA method. RESULTS: (1) The serum levels of hs-CRP and TNFalpha in the patients with ACS were significantly higher than those in the control group (P < 0.05). (2) The serum levels of hs-CRP and TNFalpha significantly lowered after one week of therapy in the two fluvastatin treated groups (P < 0.01 in all), especially in the 80 mg fluvastatin group, while no significant difference was observed before and after treatment in the routine therapy group. (3) The serum levels of hs-CRP and TNFalpha had a significant positive correlation in the patients with ACS (r = 0.70, P < 0.01), but no relationship were observed between TC, TG, LDL-C, or HDL-C and hs-CRP or TNFalpha by Pearson correlation analysis. CONCLUSIONS: The serum levels of inflammatory factors including CRP and TNFalpha are increased in patients with ACS and early fluvastatin intervention may decease dose-dependently the serum levels of hs-CRP and TNFalpha. The anti-inflammatory effect of fluvastatin may be beyond that of lipid lowering. Early intensive fluvastatin treatment may yield more significant benefits in the patients with ACS.

Adult↗

PubMed2005

[Effect of nuclear factor-kappaB on differentiation of bone marrow stromal cells into neurons induced by baicalin in rats].

OBJECTIVE: To explore the effect of nuclear factor-kappaB (NK-kappaB) on differentiation of bone marrow stromal cells (MSC) into neurons induced by baicalin. METHODS: Using baicalin as main inducer, differentiation of rats' MSC was induced into NC in serum-free medium, at the same time, untreated cells were cultured in serum-free medium as the control group. The expression of NC special marker protein and the presence of NK-kappaB subunits RelA (P65) translocated into nucleus were determined by immunofluorescent cytochemical stain, the change of expression of NK-kappaB inhibitory protein (IkappaBalpha) were determined by Western blot, and the apoptotic index was assessed by terminal deoxynucleotidyl transferase-mediated d-UTP-biotin nick end labeling (TUNEL) assay. RESULTS: MSC displayed typical shape of NC and expressed several NC marker protein after induced by baicalin, while those changes were not revealed in the control group. but showed translocation of P65 from cytoplasm to nucleus, and the markedly reduction of IkappaBalpha expression, the apoptotic index being 28.2+/-6.1 %. After induced by baicalin, the P65 still revealed mainly in cytoplasm with the level of IkappaBalpha expression decreased lesser and the number of apoptosis cell (12.2+/-2.8%) lower than those in the control group, P <0.01. CONCLUSION: Baicalin could inhibit NK-kappaB activation, and it has certain effect in inducing the differentiation of MSC into NC.

Animals↗

PubMed2005

[Metabonomics and its applications].

The concept, characteristics and history of metabonomics are introduced. The techniques used in data acquisition and data analysis in metabonomics including their advantages and disadvantages are summarized. In data acquisition platform, NMR, GC/MS, LC/MS (/MS) are the prevalent techniques although at present, none of them is a perfect technique that could meet with the requirement of the metabonomics for measuring all metabolites. While in data analysis, the PCA, PLS and ANN are the major techniques. The researchers could select them according to the research destination. Recent advances and applications of metabonomics in disease diagnosis, drug toxicity evaluation, plant metabolomics and microbial metabolomics are reviewed. In addition, by giving the situation on the establishment of the related corporations, the conferences about metabonomics and proclamation of NIH roadmap the current boom of the metabonomics is reflected. It can be expected that with the development of the function genomics, metabonomics will play a major role in the discovery of the phynotype of the genome and searching for the disease diagnostic biomarkers, and it will also bring much benefit to the drug discovery, clinical diagnosis and nutrition science.

Humans↗

PubMed2005

[Multidimensional separations used in pharmaceutical and biological fields].

A review of multidimensional separations such as comprehensive two-dimensional gas chromatography (GC x GC), comprehensive two-dimensional high performance liquid chromatography (HPLC x HPLC) and their applications in pharmaceutical and biological fields is presented with 71 references. A single CO2 cryo-jet loop modulator was developed for GC x GC and it can be used to modulate compounds higher than C6 effectively. Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC x GC/TOF-MS) analyses of traditional Chinese medicine volatile oils such as Pogostemon cablin Benth (Cablin Patchouli), Forsythia suspensa (Thunb.) Vahl and Zedoary were reported also. As an emerging technology, multidimensional separations hold the promise and play an important role in the future pharmaceutical and biological fields.

Animals↗

PubMed2005

[Dexamethasone pharmacokinetics in perilymph of guinea pig after different topical administrations].

OBJECTIVE: To determine pharmacokinetics profiles of dexamethasone (DEX) in perilymph of guinea pig with different intratympanic administrations, and to provide experimental evidence for clinical choice about administration. METHOD: Two different topical administrations placing a granule gelfoam soaked with DEX (10 g/L) in the niche of round window of guinea pig and infusing tympanic bulb with DEX solution (10 g/L) were conducted. Perilymph of the scala tympani was sampled 1-6 h after administration. DEX in the samples was assayed with high-performance liquid chromatography. RESULT: The concentrations of DEX in perilymph after placing a granule gel-foam soaked with DEX in the niche of round window were significantly higher than that after intratympanic infusing. The half-life time of DEX after placing a granule gelfoam was longer. CONCLUSION: DEX could effectively penetrate round window membrane into perilymph after different intratympanic administrations, and the method of placing a granule gelfoam soaked with DEX in the niche of round window is more effective than that of intratympanic infusing.

Administration, Topical↗

PubMed2005

[Allelopathic effects of Cymbopogon citratu volatile and its chemical components].

This paper studied the allelopathic effects of Cymbopogon citratus volatile on the seed germination and seedling growth of corn and barnyard grass (Echinochloa crusgalli) in field and in obturator, and analyzed the chemical components of the volatile with SPME and GC-MS. The results of bio-assay indicated that the germination rate of corn or barnyard grass intercropped with C. citrate or enclosed in obturator with fresh C. citratus had no significant difference from the control, but the seedling growth of corn and barnyard grass was significantly inhibited. The volatile from C. citratus roots contained 10 components. The main component was longifolene-(V4), occupying 56.67% of the total, the second component was selina-6-en-4-ol (20.03%), while the others were under 10%. There were 12 components in the volatile from C. citratus shoots. The main component was citral (53.98%), the second was z-citral (34.40%), and the others were under 4%. There were 2 monoterpenes and 9 sesquiterpenes in the volatile from shoots, and all the terpenes in the volatile from roots were sesquiterpenes. Therefore, the allelopathy of C. citratus should not be ignored when planted it with other crops.

Acyclic Monoterpenes↗

PubMed2005

[Carboplatin-induced injury patterns of inner hair cells missing in chinchilla cochlea].

OBJECTIVE: To explore carboplatin-induced injury patterns of inner hair cells (IHCs) in cochlea of chinchilla, namely, when injury of IHCs occurs, what extension injury of IHCs exists at different time after drug administration, where maximal injury of IHCs locates on basal membrane and how long most IHCs lose after drug administration. METHOD: Carboplatin of moderate concentration (100 mg/kg) was used to inject peritoneally one time and animals were decapitated on schedule, surface preparations were made and cochleograms were obtained to observe injury of IHCs in the cochleae of chinchillas. RESULT: Loss of outer hair cells was not observed on basal membrane from 24 hours to 3 months after drug administration except one ear. The characteristic of IHCs injury patterns was found to be: (1) IHCs were intact at 24 hours after drug administration. IHCs injury was firstly observed within 3 days. (2) The maximal rate of IHCs injury located around boundary of turn one and turn two on basal membrane from 24 hours to 3 months. (3) IHCs injury began from boundary of turn one and turn two and extended to basal turn and apical turn gradually. Compared to apical turn, IHCs injury on basal turn occurred earlier and more severely. (4) Most IHCs lost in 3 months. CONCLUSION: Carboplatin of 100 mg/kg unequally destroyed IHCs from different turns. Temporal variance of injury of IHCs from given turn is most likely due to their discrepant sensitivity to carboplatin.

Animals↗

PubMed2005

[Study on treatment strategy for ventriculitis associated with ventriculoperitoneal shunt for hydrocephalus].

OBJECTIVE: To study the treatment strategy for ventriculitis associated with ventriculo peritoneal shunt for hydrocephalus. METHODS: For all of the patients suspected to have cerebrospinal fluid (CSF) infections, the first step of treatment was removal of the "infected" shunts, then a ventricular drainage was placed in frontal horn of the ventricle, and at the same time CSF was obtained from the ventricle for bacterial culture and tests of bacterial sensitivities to antibiotics. After the shunt removal and before the result from bacterial cultures was obtained, 25-50 mg of vancomycin was administered intraventricularly daily. As soon as the result from bacterial cultures was obtained, antibiotics should be adjusted according to the sensitivity test result. By the time of the infections being controlled, appropriate treatments including reshunting would finally be given for hydrocephalus if necessary. RESULTS: Among 11 patients, 9 were infected with coagulase-negative Staphylococci or Staphylococcus epidermidis and Staphylococcus aureus, one of them with concomitant infection of aerobacter cloacae, Klebsiella pneumoniae and enterobacteriaceae aerogenesis, 1 patient with Streptococci, and another one with Mycobacterium stercoris bovis. The tests of drug sensitivity showed that cocci in infection of 9 patients were all sensitive to vancomycin, but only 1 of them sensitive to gentamycin. There were other sensitive antibiotics for streptococcus in 1 case and for bacillus in 2 patients. Practically vancomycin was effective for 8 of 11 patients, including one case of streptococcal infection. The duration for anti-infection treatment was in a range of 16-36 days. Finally, all of 11 patients survived from the shunt ventriculitis, and 9 of them had significant improvement in their neurological functions. CONCLUSION: (1)Intraventricular administration of proper antibiotics is a reliable and effective way to treat ventriculitis associated with ventriculoperitoneal shunts. (2)Vancomycin is the preferred antibiotic for ventriculitis, but other kind(s) of some antibiotics are necessary in a few patients in addition to or instead of vancomycin. (3)The effect of systemic use of antibiotics could not be defined in this series of patients, but this may be used as an adjunct in the practice. (4)There are many problems related to diagnosis and treatment of shunt ventriculitis that should be studied more extensively and deeply.

Adolescent↗

PubMed2005

[Construction of artificial nerve bridge by three-dimensional culture of Schwann cells with human hair keratins].

OBJECTIVE: To culture Schwann cells (SCs) and human hair keratins (HHKs) for artificial nerve bridge construction. METHODS: SCs were purified by primary culture and labeled with BrdU, which were then cultured with HHKs decorated by ECM. The artificial nerve bridge was implanted into the defect of sciatic nerve, beneath the skin, and in the skeletal muscles of SD rat, respectively. The morphology of the SCs cultured with HHKs was monitored by inverted microscope and evaluated by immunocytochemical staining. Growth of BrdU-labeled SCs in vivo was observed by immunocytochemical staining on paraffin sections. RESULTS: In vitro cultured SCs were capable of adhering to HHKs and grew well four weeks after implantation. The HHK component in the artificial nerve bridge underwent degradation in the defect of the sciatic nerve, beneath the skin, and in the skeletal muscles of SD rat, and SC survival and proliferation were verified. CONCLUSION: SCs can survive in three-dimensional culture with HHKs for construction of artificial nerve bridge to repair nerve defects.

Animals↗

PubMed2005

[Construction and clinical application of tissue engineered epidermal membrane].

OBJECTIVE: To Construct tissue engineered epidermal membrane for promoting healing of clinical skin graft donor site wound. METHODS: Epidermal cells /Chitosan-Gelatin (CG) membrane was constructed with cultured human epidermal cells(EC) and CG membrane, at the donor site of split skin graft, the wound was divided into three groups: the control group was covered with CG membrane without KC, KC/CG membrane was grafted on the treatment part of the wound area, and blank group was covered with traditional vaseline gauze. The engineered epidermal membrane and its effect on wound were evaluated with gross observation, HE, immunohistochemistry, collagen type I/III ratio by picrosirius polarization method and RT - PCR test at various time points before and after operation. RESULTS: The result showed that human tissue engineered epidermis could be constructed with cultured human EC and CG membrane, and this artificial epidermal membrane could be used for promoting the healing of skin graft donor site wound successfully (16 cases with 3 months' oberservation). The average healing time is (16.2 +/- 3.8) days for control group, (8.1 +/- 1.3) days for experimental group and (23.0 +/- 5.7) days for blank group. The artificial epidermis was well survived with normal structure. In addition, less hypertrophic scar development was observed in treated wound at 90 days (3 in 16 cases, 20.0%) than in the blank sites (11 in 16 cases, 74.4%). The difference is statistically significant (chi2 = 8.127, P < 0.01). CONCLUSIONS: The constructed EC-CG membrane can be constructed in vitro, survived in vivo and has good clinical application in promoting healing of skin graft donor site wound and inhibiting hypertrophic scar formation.

Adult↗

PubMed2005

[Cloning and expression of human interleukin 24 gene and study on its activity of inducing tumor cells apoptosis in vitro].

AIM: To clone and express IL-24 gene in E.coli and to study its antitumor activity to induce apoptosis of tumor cells after purification and refolding. METHODS: The total RNA was extracted from peripheral blood mononuclear cells (PBMC) and induced by ConA. The human IL-24 gene was amplified by RT-PCR before subcloned into expression vector pET32a(+) and expressed in E. coli. The fusion protein was identified by Edman and purified in chelating sepharose fast flow chromatography and refolding. The activity of inducing tumor cells apoptosis was observed by MTT colorimetry and morphological study in vitro. RESULTS: The obtained human IL-24 gene was identical with that included in GenBank. The recombinant IL-24 could be expressed in E. coli by IPTG induction as inclusion body (35 kD) with purity over 95%. The apoptosis of MCF-7 cells was induced by Trx-IL-24 protein. CONCLUSION: The fusion protein can be highly expressed in E. coli and the obtained protein with high purity can induce tumor cells apoptosis.

Apoptosis↗

PubMed2005

[Review on hrp genes of plant pathogenic bacteria].

The hrp genes exist in 4 kinds of Gram-negative plant pathogenic bacteria and are responsible for the pathogenicity of bacteria. They can induce hypersensitive response on non-host and resistant plants. In the present paper, we summarized the hrp genes clusters, the relationship between hrp and avr genes, harpin proteins encoded by hrp genes, modulation and function of hrp genes, and plant-bacteria interactions mediated by hrp genes in more details. Moreover, trends in future research of plant pathogenic bacteria hrp genes have also been analyzed.

Bacteria↗

PubMed2005

[The relaxation effects of six extracts from Chinese herbs on the corpus cavernosum tissue of rabbit in vitro].

OBJECTIVE: To study the relaxants effects of six extractions from Chinese Herbs (neferine, tetrandrine, kakonein, scutellarin, ginsenoside Rgl and ginsenoside Rb1) on the corpus cavernosum tissue of rabbit in vitro. METHODS: Isolated stripes of rabbit corpus cavernosum tissue were precontracted with 10(-5) mol/L phenylephrine (PE). Relaxation in response to cumulative doses of six extracts at (10(-8) - 10(-3)) mol/L was determined. RESULTS: On rabbit cavernosal muscle stripes precontracted with PE, neferine, tetrandrine, kakonein and scutellarin showed dose dependent relaxation. IC50 values were 4.60 x 10(-6), 3.73 x 10(-5), 8.03 x 10(-4) and 3.33 x 10(-3) mol/L, respectively. However, in the meantime, it was found that the relaxant effects of ginsenoside Rgl and ginsenoside Rbl less significant to stripes precontracted with PE. When the final concentration was 10(-3) mol/L, the relaxations were only (16.32 +/- 5.45)% and (11.21 +/- 3.10)%. CONCLUSION: Among the six extracts which showed relaxant effects to rabbit cavernosal muscle stripes precontracted with PE, neferine had greater functions than the other five extracts.

Animals↗

PubMed2005

[Oxidative stress-induced differentiation of L6 myoblasts].

OBJECTIVE: To explore the relationship between the differentiation of L6 myoblasts and oxidative stress. METHODS: MTT assay was used to determine the viability of L6 myoblasts, from which the total RNA was extracted for amplification of the myogenin gene fragment by RT-PCR. H(2)O(2)-induced morphological changes of the cells were observed. RESULTS: The myoblasts treated with low concentration of reactive oxygen (50 micromol/L H(2)O(2)) for 1 h exhibited accelerated cell growth (P<0.05), and treatment with 50 and 150 micromol/L H(2)O(2) induced the gene expression of myogenin, a molecular marker for differentiation of myoblasts. Morphological study revealed myotube formation and accelerated differentiation of the myoblasts induced by H(2)O(2). CONCLUSION: The reactive oxygen may serve as the intracellular signal molecules to induce the growth and differentiation of the myoblasts.

Animals↗

PubMed2005

[Resection of child meningoencephaloceles by transnasal endoscopy].

OBJECTIVE: To discuss the diagnosis of child meningoencephaloceles in nasal cavity and its methods of transnasal endoscopic surgery. METHOD: Two cases of congenital meningoencephalocele which have manifested mass in nasal cavity or nasopharynx received total resection by transnasal endoscopic surgery. Their medical records, radiographic imagings, and surgical procedures were reviewed. RESULT: We identified 2 patients with endonasal meningoencephaloceles whose main symptoms were watery rhinorrhea,nasal obstruction and mass in nasal cavity. A high index of suspicion for this diagnosis must be maintained, especially in patients with reiterative high fever or meningitis. Imaging examination should be done in those patients. Endonasal meningoencephaloceles, wherever the bone defects were located on the ethmoid roof or skull base,can be remove by endoscopy. CONCLUSION: CT and MRI are significant in diagnosis of endonasal meningoencephaloceles, nasal endoscopic surgery could be considered to be the best surgical approach without any severe complications.

Child, Preschool↗

PubMed2005

[Analysis on direct economic burden of stroke in the rural population of Hanzhong, Shaanxi Province].

OBJECTIVE: To analyze the direct economic burden of stroke in rural areas of Hanzhong. METHODS: Plan on primary interview was made after the purpose of the study had been informed to the managers of the 'surveillance field base', heads and members of the monitor assistants and detailed information was collected in the fields. Every single patient of stroke was then interviewed by the above said interviewers,using a self-designed questionnaire. 164 patients with stroke were interviewed in 53 villages with 75,000 persons lived there. The main items involved in the questionnaire would include: costs for inpatient or outpatient, reaching-out fees, fee for accommodation during treatment as outpatient, costs for treatment at home, long term medicine, caregivers and funerals as well as average income. RESULTS: The median of annual direct economic burden was 3100 Yuan for each patient in Hanzhong rural area. There were no significant differences seen between males and females or among age groups (P > 0.05). The proportion of patients with medians of annual direct economic burden of: 1000 Yuan and below, 1001-5000 Yuan, 5001-10,000 Yuan, 10,001-20,000 Yuan and over 20,001 Yuan, were 29.2%, 36.0%, 18.3%, 9.8% and 6.0% respectively. The median of annual direct economic burden of first episode stroke was 5500 Yuan for each patient, and that of stroke was 1700 Yuan for each chronic patient. The direct economic burden of first episode was significantly higher than that of stroke (P < 0.01). The costs of hospitalization, accommodation of hospitalization and treatment at home of middle-aged patients were significant higher than that of old age patients (P < 0.05). CONCLUSION: In this study, the direct economic burden of stroke was 2.9 times of the annual personal average income, which was contrary to the reports from other countries. However, the State Health Bureau bore 87.1% of the direct economic burden for urban patients, but patients in the rural areas had to pay from their own pockets. The direct economic burden of stroke was heavy in Hanzhong rural region, which called for measures to be made to decrease the direct economic burden of stroke in the region.

Age Distribution↗