Biomedical subjects

Jun Yue

Publications and source records attributed to Jun Yue.

11 recordsLinked to original sources

PubMed2005

Detection of mutations associated with isoniazid resistance in Mycobacterium tuberculosis isolates from China.

Nine structural genes (furA, katG, inhA, kasA, Rv0340, iniB, iniA, iniC, and efpA) and two regulatory regions (the oxyR-ahpC intergenic region and the promoter of mabA-inhA) in 87 isoniazid (INH)-monoresistant and 50 INH-susceptible Mycobacterium tuberculosis isolates collected from five provinces of China were analyzed by sequencing. Eighty-two (94.3%) INH-resistant isolates had mutations in the katG gene, with the katG Ser315Thr mutation predominant (55.2%). No mutation at codon 463 of katG was detected among the 50 INH-susceptible isolates with different IS6110 fingerprints. In addition, there were 35 (40.2%) INH-resistant isolates that had a mutation at codon 463 of katG. Of the INH-resistant strains, 20 (23.0%) isolates harbored double mutations at two separate loci of katG. Mutations in the inhA promoter region occurred in 13 (14.9%) isolates; 4.6% of the isolates had inhA structural gene mutations, and 11.5% harbored mutations in the oxyR-ahpC intergenic region. Drug resistance-associated mutations were detected in the iniBAC region and efpA.

Adolescent↗

PubMed2005

[Effects of the Radix Ranuncoli Ternati extracts on Mycobacterium tuberculosis proteome profiling revealed by 2D electrophoresis].

Radix Ranuncoli Ternati is clinically effective traditional Chinese medicine for multidrug resistant tuberculosis. Its active components and mechanism of action remain unsolved. Two dimensional gel electrophoresis (2-DE) was employed to address this problem. Globlal proteome of Mycobacterium tuberculosis untreated and treated with Radix Ranuncoli Ternati were compared, and 22 protein spots were found to be expressed differentially. 3 protein spots which remarkably decreased in Mycobacterium tuberculosis treated with Radix Ranuncoli Ternati were subjected to matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) analysis. The data obtained from peptide mass finger printing were used for database search. The 3 protein spots in gel were identified as cysA2 (thiosulfate sulfurtransferase), tsf (elongation factor EF-Ts) and hspX (heat shock protein X). These data provide insights into the changed global protein patterns of Mycobacterium tuberculosis treated with Radix Ranuncoli Ternati and may prove useful for further study in the mechanism in how Radix Ranuncoli Ternati influence the life of Mycobacterium tuberculosis. The differentially expressed proteins may be potential novel antituberculosis drug targets.

Antigens, Bacterial↗

PubMed2004

Detection of rifampin-resistant Mycobacterium tuberculosis strains by using a specialized oligonucleotide microarray.

DNA microarray represents one of the major advances in diagnostic sequencing of polymerase chain reaction (PCR) products. Until now, arrays have been relatively expensive, complex to perform, and difficult to interpret, limiting their wide application in the clinical laboratory. A moderate-density oligonucleotide microarray that can rapidly identify Mycobacterium tuberculosis rifampin-resistant strains was developed. The method is based on the detection of point mutations and other rearrangements in the rpoB gene region determining rifampin resistance. Rifampin resistance was determined by hybridizing fluorescently labeled, amplified genetic material generated from bacterial colonies to the array. Fifty-three rifampin-resistant M. tuberculosis and 15 rifampin-susceptible M. tuberculosis were tested and results were concordant with those based on culture drug susceptibility testing and sequencing. Rifampin-resistant clinical isolates were detected in as little as 1.5 hours after PCR amplification with visual results. It is demonstrated that oligonucleotide microarray is an efficient, specialized technique to implement and can be used as a rapid method for detecting rifampin resistance to complement standard culture-based method.

Antibiotics, Antitubercular↗

PubMed2004

[Differential expression of apoptosis-related gene induced by clinical and laboratory Mycobacterium tuberculosis strain in macrophages U937 revealed by oligonucleotide microarray].

Tuberculosis(TB) remains one of the major problems in global health. Macrophage (MPhi) apoptosis, induced by Mycobacterium tuberculosis (Mtb), is a cornerstone of effective innate microbial defense mechanism. Elucidation of the complex apoptosis-related gene expression may facilitate understanding the mechanism and regulation of macrophage apoptosis in response to Mtb, and contribute to developing novel measures to counter TB. DNA microarray containing 19,200 gene or gene fragments was used to compare the macrophage cell line U937 gene expression response to the clinical and laboratory Mtb infection. Northern blotting and RT-PCR were used to confirm the microarray results. Mtb H37Rv infection were found to downregulate the bcl-2, vitamin D receptor, interferon regulatory factor 3, cytochrome c oxidase, gene expression by 2-, 3-, 3-, 2.5-fold, respectively, while the clinical strain infection leads to upregulate the SOD2, SOD3, serine protease, toll-like receptor 2, signal transducer and activator (STAT1), hypoxia-inducible factor 22, 2.9-, 2.5-, 2.5-, 2.2-, 2.4-, 5.9-fold respectively. The findings suggest that the clinical strain infection tends to override the macrophage apoptosis by which the host attempt to limit the growth of the invader. The research on the complex factors network involved in the interaction will benefit the vaccine and novel drug target development.

Apoptosis↗

PubMed2004

[Molecular mutations of rpoB gene of multidrug resistant Mycobacterium tuberculosis isolates from China].

To characterize rpoB mutations of multidrug resistant Mycobacterium tuberculosis clinical isolates from China, mutations in the 81 bp rifampin resistance determining region (RRDR) and mutation V176F locating in the beginning of the rpoB gene were analyzed by DNA sequencing. Eighty six Mycobacterium tuberculosis clinical isolates, including 72 rifampin resistant strains and 14 rifampin sensitive strains were sequenced. Six five mutations of 22 distinct kinds, 21 point mutations and one insertion, were observed in 65 of 72 resistant isolates. The most frequent mutations were in codon 531 (41%), 526 (40%), and 516 (4%). Mutations were not found in seven (10%) of the resistant isolates. Six new alleles within the RRDR, along with five novel mutations outside the RRDR, are reported. None of isolates contained the V176 mutation.

Bacterial Proteins↗

PubMed2004

[Identification of trehalose-phosphate phosphatase associated with drug-resistance from culture supernatants of isoniazid-resistant Mycobacterium tuberculosis].

OBJECTIVE: To isolate and identify a new protein involved in drug-resistance from culture supernatants of isoniazid (INH)-resistant Mycobacterium tuberculosis. METHODS: Bovine albumin of Middlebrook 7H10 culture supernatants of INH-resistant Mycobacterium tuberculosis was removed using diethylamine ethylcellulose affinitive gel chromatography. A specific protein from culture supernatants of three INH-resistant Mycobacterium tuberculosis isolates was separated reproducibly by high performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide electrophoresis, and identified by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-MS). RESULTS: The protein was trehalose-phosphate phosphatase involved in the biosynthesis of trehalose of cell wall, molecular a eight 46000, isoelectric point 5.3. It could be detected repeatedly from supernatants of three INH resistant Mycobacterium tuberculosis isolates. CONCLUSION: Trehalose-phosphate phosphatase represents an excellent potential target site for chemotherapy against Mycobacterium tuberculosis and a new marker in the detection of drug resistance of Mycobacterium tuberculosis.

Animals↗

PubMed2003

[Effects of Mycobacterium tuberculosis infection on the transcriptional expression of human macrophage gene encoding ion channels and related regulatory elements].

Expression microarray was employed in this study to investigate whether the ion channels and their regulatory elements encoding genes participate in the immune response to Mycobacterium tuberculosis infection. The results of a virulent strain were compared with those of the clinically isolated strains. The data demonstrate that K(+), Na(+), Ca(2+) and Cl(-) channels and their regulatory elements, such as the G protein, receptor and second messenger, protein kinase and protein phosphatase were involved in the immune reaction. The clinical strain affected more types of ion channels and respective regulatory elements. The data provides clues for further scrutiny into the role of ion channels and related elements in the interaction between Mycobacterium tuberculosis and host macrophage.

Gene Expression Regulation↗

PubMed2003

Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis isolates from China.

Mutations in the 81-bp rifampin resistance determining region (RRDR) and mutation V176F locating at the beginning of the ropB gene were analyzed by DNA sequencing of 86 Mycobacterium tuberculosis clinical isolates (72 resistant and 14 sensitive) from different parts of China. Sixty-five mutations of 22 distinct kinds, 21 point mutations, and 1 insertion were found in 65 of 72 resistant isolates. The most common mutations were in codons 531 (41%), 526 (40%), and 516 (4%). Mutations were not found in seven (10%) of the resistant isolates. Six new alleles within the RRDR, along with five novel mutations outside the RRDR, are reported. None of isolates contained the V176 mutation.

Alleles↗

PubMed2003

Using a cDNA microarray to study cellular gene expression altered by Mycobacterium tuberculosis.

OBJECTIVE: To examine the global effects of Mycobacterium tuberculosis (M. tuberculosis) infection on macrophages. METHODS: The gene expression profiling of macrophage U937, in response to infection with M. tuberculosis H(37)R(a), was monitored using a high-density cDNA microarray. RESULTS: M. tuberculosis infection caused 463 differentially expressed genes, of which 366 genes are known genes registered in the Gene Bank. These genes function in various cellular processes including intracellular signalling, cytoskeletal rearrangement, apoptosis, transcriptional regulation, cell surface receptors, cell-mediated immunity as well as a variety of cellular metabolic pathways, and may play key roles in M. tuberculosis infection and intracellular survival. CONCLUSIONS: M. tuberculosis infection alters the expression of host-cell genes, and these genes will provide a foundation for understanding the infection process of M. tuberculosis. The cDNA microarray is a powerful tool for studying pathogen-host cell interaction.

Cells, Cultured↗

PubMed2003

[Trabeculectomy with amniotic membrane transplantation and combining suture lysis of scleral flap in complicated glaucoma].

OBJECTIVE: To study and evaluate the outcome of trabeculectomy with amniotic membrane transplantation combining suture lysis of scleral flap in complicated glaucoma. METHODS: This operation was performed in 48 patients (67 eyes) with complicated glaucoma. Amniotic membrane was implanted under the scleral flap and the conjunctival flap and combined with suture lysis for scleral flap to control the outflow of the aqueous humor. RESULTS: Follow-up period ranged from 5 to 38 months (mean 19.5 months). The intraocular pressure (IOP) was lowered from (43.86 +/- 7.12) mm Hg (preoperative IOP) to postoperative (19.63 +/- 2.57) mm Hg (postoperative IOP) in neovascular glaucoma (t = 4.96, P < 0.001); from (40.31 +/- 4.79) mm Hg to (18.09 +/- 2.21) mm Hg in glaucoma after IOL implant (t = 3.54, P < 0.05); from (37.94 +/- 5.63) mm Hg to (20.14 +/- 3.15) mm Hg in aphakic glaucoma (t = 5.12, P < 0.05); from (32.48 +/- 3.98) mm Hg to (16.54 +/- 1.84) mm Hg in juvenile glaucoma (t = 4.23, P < 0.01); from (36.01 +/- 4.13) mm Hg to (18.11 +/- 3.40) mm Hg in uveitic glaucoma (t = 4.47, P < 0.01); from (34.43 +/- 5.28) mm Hg to (18.31 +/- 1.52) mm Hg in glaucoma after failure of conventional filtering operation (t = 2.05, P < 0.05). Statistical significant difference was found in the IOP before and after the operation. The rate of formation of functional bulb was 80.6%. There were no reject reaction and severe complications. CONCLUSION: Trabeculectomy with amniotic membrane transplantation combining suture lysis of scleral flap is a useful method in complicated glaucoma.

Adolescent↗

PubMed2003

[Differential expression of human macrophage genes encoding cytokines and their regulatory elements after Mycobacterium tuberculosis infection].

To investigate whether the genes encoding cytokines and their regulatory elements participated in the immune response to Mycobacterium tuberculosis infection. Expression microarray was employed to compare the avirulent strain and clinically isolated strains infection induced macrophage cytokine differential expression. Results were cytokines IFN, TNF, TGF, IL and their regulatory elements are involved in the immune reaction. IL-19 was first reported to be involved in the anti-Mtb immunity. Relative expression level of theses factors before and after infection were assayed too. The data provides clues for further scrutinize the role of cytokines and related elements in the interaction between Mycobacterium tuberculosis and host macrophage.

Cytokines↗