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Jun-Mo Yang

Publications and source records attributed to Jun-Mo Yang.

43 records · Page 3Linked to original sources
PubMed2003

Species identification and strain differentiation of dermatophyte fungi using polymerase chain reaction amplification and restriction enzyme analysis.

BACKGROUND: Standard biochemical tests, microscopy, colony characteristics, and mating tests have conventionally been used for the identification of dermatophytes species, but these methods of identification are costly, time-consuming, and require special skills. OBJECTIVE: Our purpose was to identify a method that enables rapid species identification and strain differentiation of dermatophyte fungi. METHODS: We chose 4 restriction enzymes (BsYiI, DdeI, HinfI, and MvaI) that could produce different fragment patterns after enzyme digestion according to species or strain. We performed enzyme digestions after polymerase chain reaction amplification of internal transcribed spacer region and identified different restriction fragment length polymorphisms (RFLP) according to species and strains. RESULTS: All the species included in this study could be easily differentiated using any combination of 2 different restriction enzymes except Trichophyton rubrum and T raubitschekii, which produced identical digestion patterns after all 4 restriction enzyme digestions. In the case of T mentagrophytes, MvaI and DdeI each produced 2 distinct RFLP patterns. CONCLUSION: This study showed that internal transcribed spacer region analysis using polymerase chain reaction-RFLP through DdeI and MvaI is useful for rapid identification of the majority of dermatophytes species. However, there were 2 different band patterns by DdeI and MvaI restriction enzyme digestion and no correlations between morphologic types and RFLP patterns in T mentagrophytes.

Arthrodermataceae↗
PubMed2003

Analytical electron microscopy study of nanometre-scale oxide formed in contact-hole-bottom Si surfaces.

Nanometre-scale interfacial oxides formed in contact-hole-bottom Si surfaces were investigated by analytical electron microscopy coupled with a field-emission transmission electron microscope. The results showed that the chemical state of the residual oxide formed during reactive-ion etching was mostly changed from the suboxide of Si2+ or Si3+ to the oxide of Si4+ by the following light-etch treatment. Consequently, the process of removing the residual oxide by light-etch treatment was improved and it contributed to the accomplishment of lower contact resistance.

Letter↗
PubMed2003

Keratin 9 gene mutations in five Korean families with epidermolytic palmoplantar keratoderma.

Epidermolytic palmoplantar keratoderma (EPPK) is an autosomal dominant disease characterized clinically by localized palmoplantar thickening and histopathologically by granular degeneration of the epidermis. Recent molecular biological studies have revealed that EPPK is caused by mutations of the keratin 9 gene in sequences mainly encoding the highly conserved 1 A rod domain. Here we demonstrate a novel mutation of N160H (position 8 of the 1 A domain) and two other previously reported mutations, R162W and N160S, in five unrelated Korean families with EPPK. The three-dimensional structure of the 1 A domain of the related vimentin intermediate filament protein chain is now known. Based on its likely similarity to the keratin 9 chain, we predict that inappropriate amino acid substitutions in position 10 of 1 A will likely interfere with coiled-coil dimer stability, and those in position 8 will interfere with tetramer stability. Accordingly, these mutations compromise the structural integrity of the keratin intermediate filaments leading to the pathology of EPPK.

Adolescent↗
PubMed2003

Comparison of transepidermal water loss, capacitance and pH values in the skin between intrinsic and extrinsic atopic dermatitis patients.

Atopic dermatitis (AD), with the prevalence rate of around 10 to 15%, is characterized by an intensely pruritic skin lesions with typical distribution and morphology. Recently, AD is divided into extrinsic type (ADe) and intrinsic type (ADi) according to the laboratory findings and associated diseases. ADe is well-known for high IgE level, positive response to food- or aero-allergens, whereas ADi has clinically similar skin lesions and distribution patterns of AD with normal serum IgE levels, negative in vitro test for environmental or food allergens and without associated atopic diseases. To instrumentally evaluate the differences of skin involvement and functions between ADi and ADe, we checked the transepidermal water loss (TEWL), capacitance and pH in both types of childhood AD and age-matched control. The proportion of ADi was around 20% in all AD patients (10/51). Our experiment suggested possible differences between ADi and ADe. Antecubital fossa is a famous involvement site of childhood type of AD, where both types of AD patients showed higher TEWL and decreased capacitance. ADe patients showed increased TEWL in all sites and lower hydration in 4 sites, whereas ADi patients showed no significant differences of TEWL and hydration in forehead, cheek, and back of leg.

Adolescent↗