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Biomedical subjects

Junji Tagami

Publications and source records attributed to Junji Tagami.

At least 37 records · Page 2Linked to original sources

Shear bond strength of tooth-colored indirect restorations bonded to mid-coronal and cervical dentin.

To investigate the bonding of tooth-colored indirect restorations to cervical dentin, shear bond strengths of bonded resin inlays to cervical dentin and mid-coronal dentin were investigated and compared. Indirectly fabricated composite inlays (Estenia) were cemented with a dual-cured resin cement (Panavia Fluoro Cement II) to either cervical or mid-coronal dentin. Resin cement was cured with or without light irradiation for 20 seconds. After 24-hour or one-week storage, the bonded inlays were subjected to a micro-shear bond test. The light-cure method showed significantly higher bond strengths to both dentin regions compared with the self-cure method. Further, significant improvement in bonding after one-week storage was found in the case of light-cure method rather than the self-cure method. Although the cervical dentin tended to show lower bond strength than mid-coronal dentin, significant difference between the two dentin regions was only found when the resin cement was light-cured and stored for only one day.

Analysis of Variance↗

Coronal leakage inhibition in endodontically treated teeth using resin-coating technique.

The purpose of this study was to evaluate coronal leakage inhibition using a resin-coating technique after endodontic treatment. Thirty-six human incisors were cut at the cementoenamel junction, and endodontic obturations were performed. The specimens were randomly divided into three groups according to post space preparation (10 mm depth), resin coating, and temporization. For the resin coating, the dentin surface was coated with either a combination of Clearfil SE Bond and Protect Liner F (SE/PLF) or RZII (RZ). Then, the specimens were stored in 37 degrees C distilled water for 24 hours and placed in 1% methylene blue solution for 48 hours. After which, the specimens were sectioned faciolingually along the root canal and the length of dye penetration was measured from the cementoenamel junction. Three-way ANOVA revealed that the dye penetration scores were influenced by post space preparation, resin coating, and temporization. Resin coating with RZ significantly reduced the dye penetration score and SE/PLF completely eliminated dye penetration.

Analysis of Variance↗

Regional bond strengths of a dual-cure resin core material to translucent quartz fiber post.

PURPOSE: To evaluate the microtensile bond strength (muTBS) of a dual-cure resin core material to different regions of translucent quartz fiber post in a post cavity using different surface treatments. METHODS: 30 translucent quartz fiber posts (Light-Post) were used and divided into six groups according to the surface treatments: (1) no surface treatment (Control); (2) photo-cure bonding agent, Clearfil Liner Bond 2V Bond A (PLB); (3) dual-cure bonding agent, Clearfil Liner Bond 2V Bond A+B (DLB); (4) BdA+B followed by light-cured for 20 seconds (DLB & LC); (5) silane coupling bonding agent, Clearfil Photobond with Porcelain Bond Activator (PB+PBA); (6) PB+PBA followed by light-cure for 20 seconds (PB+PBA&LC). Treated post were cemented into artificial post cavities using a dual-cure composite core material (Clearfil DC Core) and light-cured for 60 seconds from the top of the cavity. After 24-hour storage in water, each specimen was serially sliced into twelve 0.6 x 0.6 mm-thick beams for the muTBS test. The data were divided into three regions (upper/middle/bottom) and analyzed using two-way ANOVA and Dunnet's T3 multiple comparisons (alpha = 0.05). RESULTS: The highest bond strength was present in the silane coupling bonding agent group for all regions (P < 0.05). Application of the bonding agent to the post surface significantly improved the bond strength compared with control (P < 0.05). There were no significant differences in muTBS at all regions between the photo and dual-cure type bonding agents (P < 0.05). The bond strength significantly decreased at the bottom region when the post surface was treated with bonding agents (P < 0.05), whereas no regional differences in bond strength were found in the silane coupling bonding agent group (P > 0.05).

Composite Resins↗

Evaluation of the light intensity of high intensity light units.

PURPOSE: To determine the intensity of PAC (plasma arc curing) light compared with conventional QTH (Quartz tungsten halogen) light using a hardness test. METHODS: The spectral distribution of two light sources was analyzed with a spectroradiometer, after the light intensity was determined. AP-X composite was packed into a black mold (4 mm in diameter and 4 mm in depth) to prepare a cylindrical specimen. The irradiating conditions for the PAC unit in the current study were fixed at 6-second irradiation with 600 mW/cm2 (P600-6), 900 mW/cm2 (P900-6), 1200 mW/cm2 (P1200-6), 1500 mW/cm2 (P1500-6), 1800 mW/cm2 (P1800-6) and 40-second irradiation with 600 mW/cm2 (P600-40). The conventional irradiating condition for QTH was 600 mW/cm2 for 40 seconds (Q600-40). After storage in 37 degrees C water for 24 hours, the hardness of the resin composite was measured with a nanoindentation testing machine. Triplicate readings were made at a distance of every 0.5 mm down to 3.0 mm from the top irradiated surface. The comparison of means was statistically analyzed applying one-way ANOVA and Fisher's PLSD test at a significant level of P< 0.05. RESULTS: Although the peak height of the PAC lamp was different due to the light intensity, the waveforms were parallel with one another and the wavelength of peaks was located at the same number. The hardness value at a depth of 2.0 mm and at values of P1800-6 was not significantly different from the control.

Analysis of Variance↗

Bonding durability of a self-etching primer system to normal and caries-affected dentin under hydrostatic pulpal pressure in vitro.

PURPOSE: To evaluate the bonding durability of a self-etching primer system to normal and caries-affected dentin under hydrostatic pulpal pressure. METHODS: 18 extracted human molars with occlusal caries were used. Their occlusal dentin surfaces were ground flat to expose normal and caries-affected dentin using #600 SiC paper under running water. Clearfil SE Bond was placed on the dentin surface including the caries-affected dentin according to the manufacturer's instructions and then the crowns were built up with resin composite (Clearfil AP-X) under either a pulpal pressure of 15 cm H2O or none (control). The bonded specimens were stored in 100% humidity for 1 day (control) or for 1 week and 1 month with hydrostatic pulpal pressure. After storage, the specimens were serially sectioned into 0.7 mm-thick slabs and trimmed to an hour-glass shape with a 1 mm2 cross-section, isolated by normal or caries-affected dentin, and then subjected to the micro-tensile bond test. Data were analyzed by two-way ANOVA and Tukey's test (P< 0.05). RESULTS: Hydrostatic pulpal pressure significantly reduced the bond strength to normal dentin after 1-month storage (P< 0.05), but did not affect the bond strength to caries-affected dentin.

Analysis of Variance↗

Pulpal response to a newly developed MMA based resin cement for bonding tooth-colored indirect restorations.

PURPOSE: To evaluate the pulpal responses to a newly-developed MMA-based self-etch resin cement, when used as a luting agent for indirect resin composite restoration, and to compare the results with those obtained from a total-etch luting agent, glass-ionomer cement, and amalgam restoration. METHODS: 120 cervical cavities were prepared in monkey teeth and divided into four equal groups according to the restorative materials used: (1) the cavities were restored with resin composite inlays using a self-etch resin cement as a luting agent (M-Bond); (2) the cavities were also restored with resin composite inlays but using a total-etch resin cement as a luting agent (Super-Bond C & B); (3) the cavities were directly restored with glass-ionomer cement (Fuji II); or (4) the cavities were directly restored with amalgam (Dispersalloy). The restored teeth were extracted at 3, 30, or 90 days after restoration, then fixed in 10% neutral buffered formalin. The specimens were prepared using routine histopathological procedures. Five microm-thick sections were stained with hematoxylin and eosin or Brown & Brenn gram stain for bacterial observations. Histological responses in the pulpal tissue and bacterial penetration were observed under a light microscope and evaluated using standard scores. The results were statistically analyzed using the Kruskal-Wallis test (P< 0.05). RESULTS: At all time intervals, no significant differences of pulpal inflammatory responses between M-Bond and Super-Bond C&B were observed (P> 0.05). Both resin cements showed no serious pulpal responses, such as necrosis or abscess formation. In general, both MMA-based resin cements showed similar pulpal responses to those of glass-ionomer cement except for congestion of pulpal blood vessels at 3 days after restoration in which glass-ionomer cement exhibited a lower level than that of the MMA-based resin cements. For the group restored with amalgam, at 3 days after restoration, severe odontoblastic disorders and blood vessel congestions with a large infiltration of inflammatory cells were detected. At 30 and 90 days after restoration, slightly inflammatory irritations were observed irrespective of the materials used. Reparative dentin formation and bacterial penetration were found mostly in the group restored with amalgam.

Analysis of Variance↗

Effects of saliva contamination on dentin bond strength using all-in-one adhesives.

PURPOSE: To evaluate the effects of saliva contamination on microtensile bond strengths of all-in-one adhesives to dentin. MATERIALS AND METHODS: Flat superficial occlusal dentin surfaces of extracted human molars were used and randomly divided into 2 groups depending on two all-in-one adhesives: One-Up Bond F Plus (Tokuyama Dental) and Adper Prompt L-Pop (3M ESPE). For each adhesive, the teeth were allocated to 7 subgroups in which one group was bonded without contamination with saliva, serving as a control. The other groups, either before or after the polymerization of adhesives, were contaminated with saliva and dried; contaminated, dried, and adhesives re-applied; contaminated, rinsed, and the adhesives re-applied. Resin composite was then placed on the treated dentin surfaces. After 24 h storage in distilled water at 37 degrees C, the teeth were sectioned perpendicular to the adhesive interface in x and y directions, obtaining multiple beam-shaped specimens with a cross-sectional area of approximately 1.0 mm2. Specimens were stressed in tension at 1 mm/min until rupture of the bond. Data were analyzed using one-way ANOVA and the Games-Howell test. RESULTS: For both all-in-one adhesives, saliva-contaminated groups showed lower bond strengths than the controls. Re-application of the adhesives after drying or rinsing the saliva off restored the dentin bond strengths to the control levels. CONCLUSION: Saliva contamination reduced the dentin bond strengths of all-in-one adhesives. Supplementary application of the adhesive after cleaning the saliva from the dentin surface is an empirical recommendation for restoring bond strength.

Adhesives↗

Migration and particle clearance from hard-setting Ca(OH)2 and self-etching adhesive resin following direct pulp capping.

PURPOSE: To evaluate the migration and particle clearance from a hard-setting calcium hydroxide (HSCH) and self-etching adhesive resin (SEAD) following direct pulp capping using the light and electron microscope. METHODS: Exposed monkey pulps were capped with a hard-setting calcium hydroxide (Dycal) or adhesive resin (Clearfil SE Bond), and histopathologically evaluated at 14 and 21 days using light and transmission electron microscopy (n = 14). RESULTS: At 14 days, both HSCH and SEAD materials showed no severe inflammatory reactions of the pulp (necrosis and abscess formation). The main reaction was slight inflammatory cell infiltration consisting of leukocytes. A number of HSCH particles were entrapped by macrophages and observed in the small capillaries similar to blood or lymphatic vessels. For SEAD, slight hemorrhage was observed at the exposed surface. At 21 days, for both HSCH and SEAD, a few cases showed minimal inflammatory response which was limited to the area beneath the exposure. Some macrophages entrapping the HSCH particles in vacuoles within the cytoplasm were arranged at the surface of the capping layer. HSCH particles were also observed in the vessels similar to blood or lymphatic vessels. A few macrophages entrapped filler-like particles of SEAD adjacent to the capping material, but there was no evidence of any SEAD in the vessels.

Animals↗

Effect of prolonged photo-irradiation time of three self-etch systems on the bonding to root canal dentine.

OBJECTIVES: To evaluate the effect of photo-irradiation time to the adhesive on the regional bond strength of a dual-cure resin core material to root canal dentine using photo and dual-cure adhesives with self-etching primer. MATERIALS AND METHODS: Post spaces were prepared in extracted premolars and then the root canal dentine was treated with one of the following bonding procedures: (1) Clearfil SE Bond Primer/Bond (SE), (2) Nano-Bond Primer/Photo-cure adhesive (PNB), (3) Nano-Bond Primer/Dual-cure adhesive (DNB). Photo-irradiation was performed for 10 or 20s from a coronal direction. The post spaces were then filled with a dual-cure composite resin (Build-It FR) and light-cured for 60s. After 24h storage, each specimen was serially sliced into 8, 0.6 x 0.6mm-thick beams for the microTBS test. The bond strength data were divided into coronal and apical regions and analysed using three-way ANOVA and Games-Howell multiple comparison (alpha=0.05). RESULTS: The microTBS of the photo-cure adhesive resin, SE and PNB, significantly decreased (p<0.05) at the apical region when the photo-irradiation time was 10s. However, the bond strength of the SE group was significantly improved at both regions when photo-irradiation time was extended to 20s (p<0.05). There were no differences in microTBS of the photo-cure adhesive resin (PNB) cured for 20s and dual-cure adhesive resin (DNB) (p>0.05). CONCLUSION: Photo-cure adhesive was effective for application on root canal dentine when the photo-irradiation time was sufficient. Extension of photo-irradiation time to the adhesive improved the bond strength depending on the type of adhesive resin.

Adhesiveness↗

Microtensile bond strength of a filled vs unfilled adhesive to dentin using self-etch and total-etch technique.

OBJECTIVE: The purpose of this study was to evaluate the effect of a filled adhesive (One-Step Plus; Bisco) versus an unfilled adhesive (One-Step; Bisco) on the microtensile bond strength (muTBS) to dentin using total-etch (Uni-etch; Bisco) and self-etch (Tyrian SPE; Bisco) techniques. METHODS: Twenty extracted human third molars were ground flat to expose occlusal dentin. After the dentin surfaces were polished with 600-grit SiC paper, the teeth were randomly assigned to four groups according to the bonding agent and technique being used. Dentin surfaces were bonded with One-Step Plus+total-etch; One-Step Plus+self-etch; One-Step+total-etch and One-Step+self-etch. Composite buildups were performed with Clearfil AP-X (Kuraray Medical). Following storage in distilled water at 37 degrees C for 24 h, the bonded specimens were serially sectioned into 0.7 mm-thick slabs and then trimmed to hour-glass shapes with a 1 mm2 cross-sectional area (n=20). Microtensile bond strengths were determined using the EZ-test (Shimadzu) at a cross-head speed of 1 mm/min. Data were analyzed using two-way ANOVA and Tukey's post hoc test. RESULTS: There were no significant differences in the muTBS between One-Step Plus and One-Step adhesives when they were used with the total-etch and self-etch techniques (p>0.05). However with the total-etch technique both adhesives yielded significantly higher bond strength values than the self-etch technique (p<0.001). CONCLUSION: The filled adhesive One-Step Plus did not show any beneficial effect than the unfilled adhesive One-Step on the muTBS to dentin with total-etch and self-etch techniques. Irrespective from the adhesive type, self-etch technique revealed lower bond strengths than the total-etch technique.

Adhesives↗

Materials chemistry: a synthetic enamel for rapid tooth repair.

The conventional treatment of dental caries involves mechanical removal of the affected part and filling of the hole with a resin or metal alloy. But this method is not ideal for tiny early lesions because a disproportionate amount of healthy tooth must be removed to make the alloy or resin stick. Here we describe a dental paste of synthetic enamel that rapidly and seamlessly repairs early caries lesions by nanocrystalline growth, with minimal wastage of the natural enamel.

Biocompatible Materials↗

Elemental distributions and microtensile bond strength of the adhesive interface to normal and caries-affected dentin.

The aim of this study was to evaluate the microtensile bond strength (microTBS) and the elemental contents of the adhesive interface created to normal versus caries-affected dentin. Extracted human molars with coronal carious lesions were used in this study. A self-etching primer/adhesive system (Clearfil Protect Bond) was applied to flat dentin surfaces with normal and caries-affected dentin according to the manufacturer's instructions. After 24 h water storage, the bonded specimens were cross-sectioned and subjected to a microTBS test and electron probe microanalysis for the elemental distributions [calcium (Ca), phosphorus (P), magnesium (Mg), and nitrogen (N)] of the resin-dentin interface after gold sputter-coating. The microTBS to caries-affected dentin was lower than that of normal dentin. The demineralized zone of the caries-affected dentin-resin interface was thicker than that of normal dentin (approximately 3 microm thick in normal dentin; 8 microm thick in caries-affected dentin), and Ca and P in both types of dentin gradually increased from the interface to the underlying dentin. The caries-affected dentin had lost most of its Mg content. The distributions of the minerals, Ca, P, and Mg, at the adhesive interface to caries-affected dentin were different from normal dentin. Moreover, a N peak, which was considered to be the collagen-rich zone resulting from incomplete resin infiltration of exposed collagen, was observed to be thicker within the demineralized zone of caries-affected dentin compared with normal dentin.

Dental Caries↗

Micro-shear bond strength and morphological analysis of a self-etching primer adhesive system to fluorosed enamel.

PURPOSE: The aim of this study was to evaluate micro-shear bond strength and morphological analysis of a self-etching primer adhesive system to fluorosed enamel. MATERIALS AND METHODS: Extracted human molars were classified according to the severity of fluorosis using Thylstrup and Fejerskov index into four groups (TFI: 0, normal; 1-3, mild fluorosis; 3-6, moderate fluorosis; 6-7, severe fluorosis) and divided into following two sub-groups. For the first sub-group, a self-etching primer adhesive system was applied to the ground enamel surfaces and the other sub-group was conditioned with 37% phosphoric acid for 30s prior to application of the same adhesive system. Teeth were then restored with a resin composite, stored for 24h in water and micro-shear bond strengths were measured. After shear testing, the fracture modes were observed under a laser scanning microscope. Morphological study of etching patterns and adhesive interface was done under a scanning electron microscope (SEM). The data were analyzed using two-way ANOVA and Sheffe test (p=0.05). RESULTS: No statistically significant difference was there between the different degrees of fluorosis in each sub-group. However, significant difference in the bond strengths between phosphoric acid etching and self-etching was found in moderate and severe groups. The SEM observations showed at the resin-enamel interface, thick resin tag like extensions penetrated around 3.5 microm into the enamel etched with phosphoric acid, while self-etching primer created, 1 microm lamina like penetration. CONCLUSION: Severity of fluorosis affects the micro-shear bond strength of a self-etching bonding system to fluorosed enamel.

Acid Etching, Dental↗

Effect of hybrid layer on stress distribution in a premolar tooth restored with composite or ceramic inlay: an FEM study.

The aim of this finite elemental stress analysis study was to evaluate the effect of hybrid layer on distribution and amount of stress formed under occlusal loading in a premolar tooth restored with composite or ceramic inlay. The mandibular premolar tooth was selected as the model based on the anatomical measurements suggested by Wheeler. The analysis is performed by using a Pentium II IBM compatible computer with the SAP 2000 structural analysis program. Four different mathematical models including the following structures were evaluated: 1) composite inlay, adhesive resin, and tooth structure; 2) composite inlay, adhesive resin, hybrid layer, and tooth structure; 3) ceramic inlay, adhesive resin, and tooth structure; 4) ceramic inlay, adhesive resin, hybrid layer, and tooth structure. Loading was applied from the occlusal surface of the restoration, and shear stresses under loading were evaluated. The findings were drawn by the Saplot program, and the results were analyzed by graphical comparison method. The output indicated that the hybrid layer acts as a stress absorber in models 2 and 4. The hybrid layer has also changed mathematical values of stress on cavity floors in both restoration types. Ceramic inlay collected the stress inside the body of the material, but the composite inlay directly transferred the stress through dental tissues. As a result, it was concluded that the hybrid layer has an effect on stress distribution under loading in a premolar tooth model restored with composite or ceramic inlay.

Acrylic Resins↗

Effect of acidic pretreatment combined with a silane coupling agent on bonding durability to silicon oxide ceramic.

This study examined the effect of different acidic treatments and the role of a phosphate monomer in a silane coupling agent on the durability of the dual-cure resin cement/silicon oxide bond. Ceramic blocks (Vita Celay Blanks) were cut into multiple 3 mm-thick slices and polished using 600 grit SiC paper. Two pairs were left untreated [controls (CTRL)], two pairs were treated with 40% phosphoric acid and rinsed with water for 30 s (PA), and another two pairs treated with 20% hydrofluoric acid followed by 30 s water rinsing (HF). Half the specimens were silanated with Tokuso Ceramics Primer (TCP) (Tokuyama) and the other half with TCP formulated without phosphate monomer (TCP-NoPM). All the pairs were bonded with Bistite II dual-cure resin cement (Tokuyama) and light cured. After 24 h water storage at 37 degrees C, 0.7 mm-thick slabs were serially sectioned. Immediately, after 6 months and after 1 year of water storage, two slabs were randomly selected from each subgroup, and sliced into beams (6 x 0.7 x 0.7 mm) for the microtensile bond strength (muTBS) test. The muTBS data were statistically analyzed using multiple Wilcoxon Signed Rank tests (p < 0.05). Failure modes were determined using a confocal laser-scanning microscope. Ceramic surface morphology after the different acidic treatments was examined using an SEM. After 1 day, in the case of silane treatment with TCP, there were no significant differences in muTBS between the control and acid-treated groups (p > 0.05), whereas with TCP-NoPM, the muTBS of the control was significantly lower than the acid-treated groups (p < 0.05). All the TCP and acid-treated TCP-NoPM groups exhibited significant reductions in muTBS after 6 months (p < 0.05). After 1 year, the muTBS of the acid-treated TCP groups were not significantly different from the control TCP group (p > 0.05). There was also no significant difference between the HF-treated TCP and TCP-NoPM groups (p > 0.05) after 1 year, all exhibiting greater than 10 MPa tensile bond strength. It is suggested that acidic pretreatment of the ceramic surface does not improve the durability of the dual-cure resin cement/silicon oxide ceramic bond when an acidic phosphate monomer is present as an activator in a ceramic primer.

Acid Etching, Dental↗

Seven-year dentin bond strengths of a total- and self-etch system.

The aim of this study was to determine the durability of tensile bond strengths of a conventional 3-step bonding system (Superbond D-liner Plus) and a self-etching priming bonding system (Clearfil Liner Bond II) to bovine dentin over a 7 yr period. Superficial bovine dentin finished with 600-grit SiC paper was bonded with one of the two adhesive materials. A 4 mm diameter area was bonded, covered with resin composite and stored in 37 degrees C deionized water containing gypsum chips and 0.4% sodium azide as an antibacterial. Bonds were stressed in tension at a crosshead speed of 1 mm min(-1). Mean bond strengths were observed at 1 d, at 1, 3, and 6 months, and at 1, 2, 3, 6 and 7 yr. Ten specimens were tested for the first 3 yr and 15 specimens were tested for 6 and 7 yr. The bond strength of Superbond D-liner Plus significantly decreased over the 7 yr period, whereas Liner Bond II showed a slight but, insignificant, decrease. The fracture mode changed with time for Superbond D-liner Plus, but not for Liner Bond II. It was concluded that systems which use a strong acid for demineralizing the dentin may show greater deterioration of the bond to dentin compared with a self-etching priming system.

Acid Etching, Dental↗

Pulpal response to a hybrid composite resin inlay bonded with a newly developed resin-modified glass ionomer luting cement.

This study evaluated the pulpal response of hybrid composite resin inlay luted with a resin-modified glass ionomer cement, and compared it with a glass ionomer cement and an amalgam. Cervical cavities were prepared in monkey teeth. A resin-modified glass ionomer luting cement (Ionotite F, Tokuyama Dental Corp.) was applied to the teeth in one of the experimental groups, and then hybrid composite resin inlays (Estenia, Kuraray Medical Inc.) were bonded to the cavities. The teeth were extracted after 3, 30, and 90 days and stained with Hematoxylin and Eosin staining or Brown and Brenn gram stain for bacterial observation. No serious inflammatory reaction of the pulp, such as necrosis or abscess formation, was observed in any of the experimental groups. No bacterial penetration along the cavity walls was detected in the resin-modified glass ionomer luting cement group. Hence, the resin-modified glass ionomer luting cement showed an acceptable biological compatibility with monkey pulp.

Analysis of Variance↗

Reduced irradiation time in slow-curing of resin composite using an intensity-changeable light source.

Possibility to shorten the total irradiation time in slow-curing with an intensity-changeable light source, Curetron 7 (CT-7), was investigated for four hybrid-type resin composites. Irradiation condition with CT-7 was assigned as 10 or 20 seconds at low light intensity (230 mW/cm2) and followed by 20 or 10 seconds at high intensity (600 mW/cm2) respectively (i.e., a total of 30 seconds). For a reference, irradiation was carried out for 30 seconds using a halogen lamp (900 mW/cm2). After irradiation, mechanical properties of the cured composites--in terms of microhardness and flexural strength--were evaluated. Also, cavity adaptation was examined in standardized, cylindrical dentin cavities treated with an adhesive system. Compared to the reference irradiation, slow-curing with CT-7 produced acceptable mechanical properties and better adaptation. These results suggested that total exposure time of slow-curing with CT-7 could be shortened to 30 seconds.

Analysis of Variance↗