Biomedical subjects

Junko Tanaka

Publications and source records attributed to Junko Tanaka.

39 records · Page 3Linked to original sources

PubMed2002

Cytotoxic effect of nitric oxide on human hematological malignant cells.

We investigated the cytotoxic effect of nitric oxide (NO) on primary culture of human hematological malignant cells. Sodium nitroprusside (SNP), an NO donor, had cytotoxic effects on the cells of some patients with malignant lymphoma (ML), acute myelocytic leukemia (AML) or chronic myelomonocytic leukemia (CMMoL), but not with multiple myeloma. Cultured cells from the ML patient remained sensitive to SNP after the cells became resistant to anti-cancer drugs. In contrast, the cells from the patients with AML and CMMoL became resistant to SNP while anti-cancer drugs remained effective. In samples of the cells of the patients with ML and AML, the number of CD3 positive lymphoma cell was decreased by SNP and the number of CD33 negative cells and normal B lymphocytes (CD19 positive cells) were increased. In the cells of the patient with ML, apoptosis was induced by SNP. SNP had no effect on lymphocytes of healthy volunteers. These results suggest that SNP had an anti-tumor effect on human hematological malignant cells.

Adolescent↗

PubMed2002

Monitoring of urine nitric oxide (NO) related substrates and immunological competence in hematological malignancy.

It has been reported that concentrations of neopterin in the urine are changed according to the host immunological conditions. In the present study, we measured urinary concentration of neopterin in patients with malignant hematological disorders and investigated the relationship between urinary neopterin levels and laboratory indices for cellular immunity. Urine neopterin levels were correlated with serum sIL-2R levels in the patients with malignant lymphoma, and inversely correlated with lymphocyte reactivity with ConA in the patients with acute myelocytic leukemia. However, no significant correlation was observed between urine neopterin levels and lymphocyte reactivity with phytohemagglutinin and pokeweed mitogen, CD4/8 ratio, CD56+ 16+ subset or serum IFN-gamma levels. In the patients with malignant lymphoma, parallel changes in serum sIL-2R and urine neopterin were observed. The presented results suggest that urine neopterin levels are related to the activation of T cells in malignant lymphoma.

Adult↗

PubMed2002

[Catabolism of brain natriuretic peptide (BNP) in normal and nephrotic rat kidney].

The major metabolic change that characterizes nephrotic syndrome (NS) is hypoalbuminemia. Edema, which is a well-recognized clinical feature, often results in disorder of peripheral circulation and congestive heart failure. We previously reported that the albumin content of kidney lysosomal proteins was increased more than ten-fold in nephrotic rats compared to control rats, suggesting that kidney lysosomes play an important role in protein degradation in NS. The present study was carried out to elucidate the role of catabolism of BNP, which is one of the functional protein-natriuretic peptides. We injected puromysin aminonucleoside(PAN) to induce the nephrotic rat state and isolated kidney lysosomes from normal and nephrotic rat kidney cortex by our methods. Immunoblot analysis of tricine-SDS polyacrylamide gel electrophoresis of rat kidney lysosomal proteins revealed the presence of an immuno-reactive peptide band, corresponding to the endogenous BNP. In addition, this was reduced as compared with the normal control. These result suggest that kidney lysosomes play an important role in BNP metabolism to maintain body fluid homeostasis and regulate blood pressure levels.

Animals↗