Biomedical subjects

Junmei Wang

Publications and source records attributed to Junmei Wang.

23 records · Page 2Linked to original sources

PubMed2002

Development of a monoclonal antibody for neutralization of human telomerase activity.

To develop monoclonal antibodies (MAbs) to neutralize human telomerase, an epitope (hTERT(7)) in reverse transcriptase domain of hTERT was synthesized and was used to immunize BALB/c mice. Hybridomas were generated and screened by enzyme-linked immunoadsorbent assay (ELISA) for specific MAbs. One hybridoma M2 clone, isotyped IgG(1), was established. The competitive assay confirmed that the M2 antibody was hTERT(7) specific, and the affinity constant was about 1 x 10(6) M(-1). M2 could recognize cell extracts from HeLa cancer cells but not those of normal 2BS cells in ELISA assay. For in situ staining immunohistochemically, the positive staining presented in the nuclear compartment of HeLa, while 2BS was nonreactive. In TRAP-PCR ELISA, M2 markedly decreased the activities of human telomerase in HeLa cells. The sequencing of M2 heavy chain variable region proved its mouse origin. The results demonstrated that the developed mouse MAb should be hTERT specific and could not only recognize native cellular hTERT in ELISA and immunohistochemistry, but also neutralize telomerase activities. Thus, it could be hoped that the antibody could be used in clinical diagnosis and treatment of cancers.

Amino Acid Sequence↗

PubMed2002

[The effect of exercise during hemodialysis on adequacy of dialysis].

OBJECTIVE: To investigate the effect of exercise on dialysis adequacy during hemodialysis (HD). METHODS: 20 studies were performed in 20 maintenance hemodialysis patients. Each study consisted of a pair of HD treatment; the blood and dialysate flow rates, dialyser and treating time were the same. An adapted exercise cycle was attached to the dialysis bed. The patients of exercise group pedaled the exercise cycle throughout the dialysis, and had a 5 - 10 minutes rest if they needed. while the patients of a control group had no exercise. BUN, Scr and UA were measured just before and after dialysis immediately, and 60 minutes after dialysis. Furthermore, KT/V, SRI, AUR and nPCR were detected by on-line dialysis monitor. RESULTS: In the exercise group, after HD, both BUN and Scr decrease rates were significantly higher, while the rebound of BUN, Scr and UA were significantly lower than those in the control group (P < 0.05 approximately 0.01). KT/V, AUR and SRI were significant higher in the exercise group than those in the control group (P < 0.05 approximately 0.01 respectively), while nPCR showed no significant difference between the two groups. CONCLUSION: Exercise during HD is a practicable new way to improve the efficiency of dialysis.

Adult↗

PubMed2002

[Monoclonal antibodies against human telomerase reverse transcriptase: preparation, characterization, and application].

OBJECTIVE: To develop monoclonal antibodies against the catalytic subunit of human telomerase hTERT for its expression detection of human tumors. METHODS: A dominant epitope in hTERT (peptide hTERT(9))was automatically synthesized based on Fmoc method, and was used to immunize BALB/c mice. Hybridomas were generated and screened by ELISA for specific monoclonal antibodies, and the characterization of which were performed by Western blotting and immunohistochemical staining. RESULTS: Antigenic peptide hTERT(9) was synthesized and confirmed by MALDI-TOF-MS and HPLC analysis. Three hybridoma cell lines secreting anti-hTERT(9) antibodies designated as H4, G8 and A11 were established after primary screening and consequent three rounds of limited dilution. Both of H4 and G8 were IgM, while A11 was IgG1 in isotyping. The competitive assay showed that the antibodies were hTERT(9) specific, and the affinity of G8 was stronger than that of H4 and A11 assayed by affinity ranking. However, in Western blotting, both of H4 and G8 stained an about 123 000 protein band with HeLa and 293 cell extracts but not with normal 2BS cells. Besides, positive staining presented in the nucleus of HeLa, while 2BS was non-reactive immunohistochemically. The sections from paraffin-embedded blocks of 127 cases of human cancer, 40 of precancerous and 19 of benign tumors were in situ stained by G8 antibody, the results showed that the human cancer tissues were 80.31% (102/127) positive in specific nuclear reaction, on the contrary, only a minority of precancerous lesions present weak positive (17.5%, 7/40), and negative in benign tumors (0/19). CONCLUSIONS: The monoclonal antibodies developed against synthetic peptide were hTERT-specific and could recognize both the native and the denatured form. Thus their use in immunoblotting or immunohistochemistry for detecting the telomerase hTERT expression of cancer cell and tissues was promising.

Animals↗

PubMed2002

Single domain antibody to human telomerase catalytic subunit: preparation and characterization.

OBJECTIVE: To develop a recombinant single domain antibody against hTERT, human telomerase catalytic subunit. METHODS: A previously prepared His-tagged hTERT fusion protein was used as the antigen, and the variable regions in heavy chain (VH) of immunized mice were RT-PCR amplified and cloned into the pCANTAB 5E, a phagemid vector. By transfection, the display library of mouse VH was developed. The candidate clones were selected by affinity panning, and soluble VH were obtained after expression in E. coli, HB2151. The resultant single VH antibodies were characterized on their binding potentials by western blotting. RESULTS: An about 350 bp VH fragment was amplified from spleen cells of mice immunized by His-tagged hTERT and expressed by phage displayed as VH library. The size of the library was 8 x 10(4). After three rounds of affinity panning, 4 independent clones were chosen and consequently expressed as soluble single domain antibodies (Mr = 16 000). In Western blot analysis, the single domain antibody from 2 of 4 clones proved to react with the His-tagged hTERT fusion protein (Mr = 167 000) without dependence of His-tags and also detect the native hTERT (Mr = 127 000) extracted from the human HeLa cancer cell line. DNA sequencing showed both of the single domain antibodies were encoded by the heavy chain variable region of the mouse. CONCLUSIONS: The single domain antibodies developed were hTERT recognizable and hTERT specific, thus providing a basis for application of recombinant single domain antibody in inhibition of telomerase activity and anticancer therapy.

Amino Acid Sequence↗

PubMedDate not supplied

Genetic algorithm-optimized QSPR models for bioavailability, protein binding, and urinary excretion.

In this work, a genetic algorithm (GA) was applied to build up a set of QSPR (quantitative structure-property relationship) models for human absolute oral bioavailability, plasma protein binding, and urinary excretion using the counts of molecular fragments as descriptors. For a pharmacokinetic property, the consensus score of a set of models (20 or 30) was found to improve the correlation coefficient and reduce the standard error significantly. Key fragments that may boost or reduce pharmacokinetic properties were also identified. Databases searches were performed for a set of key fragments identified by bioavailability models. The percentage of hit rates of bioavailability-boosting fragments were significantly higher than those of bioavailability-reducing fragments for MDDR (MDL Drug Data Report), a database of drugs and drug leads entered or entering development. On the other hand, the opposite trend was observed for ACD (Available Chemicals Directory), a database of all kinds of available compounds.

Administration, Oral↗