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Jutta Bachmann

Publications and source records attributed to Jutta Bachmann.

5 recordsLinked to original sources
PubMed2005

Protein biochips: the calm before the storm.

The growth of protein biochip technology is on a different trajectory than other drug discovery and development technologies, such as DNA sequencing and high-throughput screening, where output per experiment has grown exponentially. By contrast, experimentation with protein biochips immediately hit barriers in output because of the limited availability of content and the challenges of running biochemical experiments on the surface of a biochip. Nevertheless, the industry has been making significant progress recently by launching new platforms with focused content and new multiplexed biochemical assays. However, this success might only represent the calm before the storm. Over the long-term, protein biochips have the potential to change the drug discovery and development process at the molecular level. The output and throughput of protein biochips could enable researchers to change from the traditional model of one target-one drug to a new model of evaluating one or more potential drugs against a panel of relevant molecular targets from a complex disease state.

Drug Design↗
PubMed2005

Protein microarrays: applications and future challenges.

Within the last decade protein microarray technology has been successfully applied for the simultaneous identification, quantification and functional analysis of proteins in basic and applied proteome research. These miniaturized and parallelized assay systems have the potential to replace state-of-the-art singleplex analysis systems. However, prior to their general application in robust, reliable, routine and high-throughput applications it is mandatory that they demonstrate robustness, sensitivity, automation and appropriate pricing. In this review, the current state of protein microarray technology will be summarized. Recent applications for the simultaneous determination of a variety of parameters using only minute amounts of sample will be described and future challenges of this cutting-edge technology will be discussed.

Animals↗
PubMed2004

Protein microarrays and multiplexed sandwich immunoassays: what beats the beads?

Protein microarray technology allows the simultaneous determination of a large variety of parameters from a minute amount of sample within a single experiment. Assay systems based on this technology are currently applied for the identification, quantitation and functional analysis of proteins. Protein microarray technology is of major interest for proteomic research in basic and applied biology as well as for diagnostic applications. Miniaturized and parallelized assay systems have reached adequate sensitivity and hence have the potential to replace singleplex analysis systems. However, robustness and automation needs to be demonstrated before this technology will finally prove suitable for high-throughput applications. Miniaturized and parallelized sandwich immunoassays are the most advanced assays formats among the different protein microarray applications. Multiplexed sandwich immunoassays can be used for the identification of biomarkers and the validation of potential target molecules. In this review an overview will be given on the current stage of protein microarray technology with a special focus on miniaturized multiplexed sandwich immunoassays.

Automation↗