Biomedical subjects

K A Hartman

Publications and source records attributed to K A Hartman.

At least 19 recordsLinked to original sources

PubMed2002

A gene coding for a novel protein specific to the olfactory rosettes of Atlantic salmon.

We have isolated a 1.6 kb clone from a cDNA library made from the olfactory rosettes of the Atlantic salmon (Salmo salar). The clone contains a 1200 bp, open reading frame (named OSC) which codes for a protein with 400 amino-acid residues (Oscp). The mRNA corresponding to OSC is strongly expressed in the olfactory rosettes and weakly expressed in gills but is expressed in only these two tissues. This suggests that Oscp may have a specific and important role in olfaction. The sequence of Oscp suggests that it is not globular. Predictions show only a small fraction of alpha-helix. Oscp is hydrophilic but with the number of positively charged residues equal to the number of negatively charged residues. No closely similar protein can be found on the basis of homology searches or hydrophobicity comparisons. However, a 44 residue segment (G300 through K343) is significantly homologous to a segment of alpha-lactalbumin (G51 through K94). The similarities include the 19 residues of the "alpha- lactalbumin-lysozyme C signature," the ten residues of the Ca(2+) binding elbow and the four cysteine residues which provide two key disulfide links in alpha-lactalbumin and lysozyme C. Two more Cys residues are also very similarly placed. We conclude that the gene OSC codes for a unique protein which most likely contains a specific site for binding Ca(2+) and plays a unique role in the signal pathway of olfaction in salmon.

Amino Acid Sequence↗

PubMed1995

Structures of poly(dG-dC) and poly(dA-dT) stabilized by anions.

Infrared spectra were used to show that the sodium salts of acetate, sulfate and phosphate (pH 7.2) selectively stabilize some of the alternative structures of poly(dG-dC).Na and poly(dA-dT).Na as a function of hydration in nonoriented gels. NaCl was used as a reference. Each anion was present at 0.36 mole per mole of nucleotide residue. The weak absorption bands from these anions did not interfere with conclusive interpretation of the IR spectra of the polynucleotides. Poly(dG-dC).Na assumed the usual B* structure with each of the anions at high hydrations (r.h. of the ambient air > or = 94%). Lowering the hydration gave the following results. With acetate, the B* structure remained with only a small fraction of a modified Z or some other unusual structure present. With sulfate or phosphate, a sharp transition to the Z structure occurred (essentially complete by 86% r.h.). With reference to chloride ions, acetate favors the B* while sulfate and phosphate (pH 7.2) favors the Z structure. Poly(dA-dT).Na assumed the usual B structure with each of the anions at high hydrations. Lowering the hydration gave the following results. With acetate, the A structure was observed at the same hydrations as with chloride. With sulfate, a sharper transition to the A structure occurred (complete by 80% r.h.). With phosphate, a still sharper transition to the A structure occurred (complete by 86% r.h.). With reference to chloride, acetate shows little difference but sulfate and phosphate (pH 7.2) promote the A over the B structure. These results are compared with past results for NaNO3.

Acetates↗

PubMed1993

Conditions for the stability of the B, C, and Z structural forms of poly(dG-dC) in the presence of lithium, potassium, magnesium, calcium, and zinc cations.

The occurrence of alternative structures for the lithium, sodium, and potassium salts of poly(dG-dC) was determined as a function of hydration using IR spectra of nonoriented gels. Poly(dG-dC).K with added KCl (r = 0.56 where r is the moles of KCl per mole of nucleotide residue) gave results essentially identical to the much studied poly(dG-dC).Na with added NaCl (r = 0.56). Both gave a sharp transition from a unique B structure (hereafter designated B*) to the Z structure upon dehydration. Poly(dG-dC).Li with added LiCl (r = 0.36) assumed the B* structure at high hydration but made a broad transition to the C structures as hydration was lowered. We believe this is the first clear evidence of the C structure for poly(dG-dC). No other structures (A, D, or Z) were observed at any hydration in nonoriented gels. Poly(dG-dC).Na with added ZnCl2 (r = 0.2) existed as a mixture of the B* and Z structures in maximally hydrated gels. A broad, incomplete transition to a higher mole fraction of Z structure occurred upon dehydration. Zn2+ promotes the Z structure for poly(dG-dC) and appears to bind to guanine residues. Poly(dG-dC).Na with added MgCl2 or CaCl2 (r = 0.2) assumed the normal B* structure at maximum hydration with no hint of Z structure. Slight dehydration produced a very sharp transition to the Z structure. Both Mg2+ and Ca2+ are strong promoters of the Z structure but do not bind to cytosine or guanine residues.

Calcium↗

PubMed1991

The existence of unique B structures in polynucleotides with alternating purine-pyrimidine sequences.

The infrared spectra of the sodium salts of calf-thymus DNA, poly(dA-dC).poly(dG-dT), poly(dA-dT) and poly(dG-dC) were measured for the samples as highly hydrated, nonoriented gels. The bands from the sugar-phosphate vibrational modes show that poly(dG-dC) assumes a B-family structure which is different from the B structures of the other samples. Poly(dG-dC) most likely assumes a wrinkled B structure. The other samples retain a smooth B structure. An alternating purine-pyrimidine sequence is not a sufficient condition for the formation of wrinkled B structure in a polynucleotide.

DNA↗

PubMed1990

Development and change of young adults' preventive health beliefs and behavior: influence from parents and peers.

A longitudinal data set is employed to explore the sources of stability and change in young adults' health beliefs and behavior concerning drinking, diet, exercise, and wearing seat belts. There is substantial change in the performance of health behaviors during the first three years of college, and peers have a strong impact on the magnitude of that change. In total, however, parents are much more important than peers as sources of influence over these beliefs and behaviors. Of the various social influence processes considered, the direct modeling of behavior appears to be the most important avenue of influence for both parents and peers. These data, along with previous papers in our research program, suggest a pattern of gradually increasing parental influence on their children's health beliefs and behavior while the children are living at home, and the persistence of that influence at least through the college years.

Adolescent↗

PubMed1989

Further explorations of common-sense representations of common illnesses.

A simple method is presented for measuring people's illness cognitions--their common-sense representations of common illnesses. Data were collected from 1,628 different respondents who described a recent illness form 1 to 3 separate times over a 17-month period. A free-clustering task performed by a set of naive participants confirmed that these cognitions fall into the five components that have been previously noted: identity, time line, consequences, cause, and cure. These five components are found to be reasonably stable over time and across different illness episodes. Several consequences of these illness cognitions, in terms of changes in health-locus-of-control beliefs and different propensities to visit a doctor, are also noted. Specifically, controllable attributions for getting sick and personal responsibility attributions for getting better are associated with increased beliefs in Self-Control Over Health and decreased beliefs in Chance Health Outcomes; people with strong Identity and Cure components in their common-sense representations of common illnesses have a greater propensity to visit a doctor when feeling ill.

Adolescent↗

PubMed1989

Existence of the C structure in poly(dA-dC).poly(dG-dT).

We show that the lithium salt of calf-thymus DNA can assume the C structure in nonoriented, hydrated gels. The transitions between the B and C structures showed little hysteresis and none of the metastable structural states which occur in oriented gels. Therefore crystal-lattice forces are not needed to stabilize the C structure. The occurrence of the alternative structures of the Li, Na and K salts of poly(dA-dC).poly(dG-dT) was measured as a function of hydration for nonoriented gels. Poly(dA-dC).poly(dG-dT).Li exists in the B structure at high hydrations and in the C structure at moderate hydrations with no A or Z structure at any hydration tested. The Na salt of poly(dA-dC).poly(dG-dT) exists in the B structure at high hydration, as mixtures of B and C at moderate hydrations and in the A structure at lower hydrations. The potassium salt behaves similarly except that mixtures of the C and A structures exist at lower hydrations. ZnCl2 and NaNO3, which promote the Z structure in duplex poly(dG-dC), promote the C structure in poly(dA-dC).poly(dG-dT). Information contained in the sequence of base pairs and not specific ionic interactions appear to determine the stability of the alternative structures of polynucleotides as hydration is changed.

Chlorides↗

PubMed1988

Structural forms and transitions of poly(dG-dC) with Cd(II), Ag(I) and NaNO3.

Infrared spectroscopy was used to study the structures and transitions in hydrated gels of double-helical poly(dG-dC) complexed with the metal carcinogens Cd(II) and Ag(I). For one Cd(II) per ten nucleotides (r = 0.1), the B structure was stable at high and moderate hydrations with D2O and the B and Z structures coexisted at low hydrations. For poly(dG-dC) with Cd(II) at r = 0.2 to 0.35, the Z structure was stable at high hydrations (94% r.h. for r = 0.2). At a given value of hydration, H2O gave a higher content of Z structure than D2O. Cd(II) most likely binds to guanine residues at N7 in both the B and Z forms of poly(dG-dC) but binding to guanine N3 can not be excluded. It is unlikely that Cd(II) binds to cytosine residues at the r values studied and the cytosine residues did not protonate at N3 as Cd(II) bound to guanine residues. Poly(dG-dC) with Ag(I) at r = 0.2 to 0.36, existed in a B-family structure which is different from the B-family structure of the type I complex of Ag(I) and calf-thymus DNA. Poly(dG-dC) with Ag(I) did not assume the Z structure at lower hydrations even though NO3- was present in the sample. Ag(I) differs from other soft-metal acids which promote the Z structure. Ag(I) most likely binds to the guanine N7 or N3 and not to cytosine residues. Cytosine residues did not protonate at N3 as Ag(I) was bound to guanine.(ABSTRACT TRUNCATED AT 250 WORDS)

Cadmium↗

PubMed1987

Structural forms and transitions for the complex of mercury(II) with poly(dG-dC).

Infrared spectroscopy was used to study hydrated double-helical poly(dG-dC) complexed with varying amounts of mercury(II). For one Hg(II) per ten nucleotide residues (r = 0.1), the B structure was stabilized and the B* structure was absent at high hydration. The Z structure did not form as hydration was reduced. For r = 0.2, the B and Z structures coexisted at high hydration and the transition to total Z structure was broad as hydration was reduced. Hg(II) was bound exclusively to the guanine residues probably at N3 or N7 for r less than or equal to 0.25. The cytosine residue did not protonate (at N3) as Hg(II) was bound to guanine. The addition of NaCl together with Hg(II) reduced the binding of Hg(II), stabilized the B structure at the highest hydration and caused a sharp transition between the B and Z structures as hydration was lowered. Hydration with D2O stabilized the Z structure for poly(dG-dC) complexed with HgCl2.

DNA↗

PubMed1986

Structural forms, stabilities and transitions in double-helical poly(dG-dC) as a function of hydration and NaCl content. An infrared spectroscopic study.

The poly(dG-dC) helical duplex forms a modified, B-family structure (B*) at very high hydration and a normal B structure at slightly lower hydration. The B* structure is slightly different in sugar-phosphate and base-stacking conformations than the B structure. Increasing the hydration or decreasing the NaCl content stabilizes B* with respect to B. Poly(dG-dC) forms the Z structure at low NaCl contents when the hydration is sufficiently reduced. At moderate NaCl content, the B to Z transition is sharp and cooperative for hydration with D2O. Hydration with H2O broadens the transition which occurs at lower hydration. This suggests that hydrogen bonding is stronger in the Z structure and helps stabilize Z over B. IR spectra may be used to quantitatively estimate the fractions of B and Z structures present in a sample. Some new indicator bands are described.

DNA↗

PubMed1986

Health as a value: methodological and theoretical considerations.

The concept of value placed on health is very important in several different theoretical approaches to the study of health behavior. In practice, however, health value is generally assumed to be universally high rather than being directly measured. If this assumption is incorrect, then theories that include health value have rarely been adequately tested. This paper presents a short 4-item Likert scale designed to measure the value placed on health. Norms from the utilization of this scale in five different samples are presented. Health value is found to increase with age among girls, but the increase apparently stops by late adolescence, before full adult levels of health value are achieved. Middle-aged women place a higher value on health than do middle-aged men, although no comparable sex difference appears in a sample of undergraduates. Consistent with theoretical predictions, both health locus of control beliefs and beliefs in the efficacy of certain preventive health behaviors correlate more highly with the performance of those same behaviors 5 to 9 months later among respondents who place a high value of health relative to those who do not value health so highly. However, this interaction is found only when it can be safely assumed that health is the primary value underlying the behavior. The importance of considering a variety of values in addition to health as possible motivators of preventive health behavior is stressed.

Adolescent↗

PubMed1985

The infrared spectrum and structure of the type I complex of silver and DNA.

Infrared spectroscopy was used to study films of the type I complex of Ag+ and DNA as a function of hydration with the following conclusions. Ag+ binds to guanine residues but not to cytosine or thymine residues. Cytosine becomes protonated as Ag+ binds to guanine. (These conclusions confirm previous models.) The type I complex remains in the B family of structures with slight modifications of the sugar-phosphate geometry. This modified B structure remains stable at lower values of hydration for which pure DNA is in the A form. Binding of Ag+ to PO2-, O-P-O or the deoxyribose oxygen is excluded.

Animals↗

PubMed1981

Structural studies of tobacco mosaic virus and its components by laser Raman spectroscopy.

Raman spectra have been recorded for oriented gels of tobacco mosaic virus, aqueous solutions of TMV protein in the form of capsid and disk, and protein-free TMV RNA. The protein-free RNA molecule contains about 80% ordered type-A structure, which is similar to other RNA molecules in solution. RNA in the virus particle does not exhibit the base stacking and pairing normally found in protein-free RNA but instead shows at least two distinct structures characterized by different phosphodiester group frequencies in the Raman spectrum. Approximately one out of three such linkages retains the type-A geometry characteristic of aqueous RNA, while two out of three appear to have a different geometry not encountered previously for RNA. The secondary structure of the TMV coat protein molecule is similar for the three aggregation states studied (virus, capsid, and disk) and consists of 40-50% alpha helix, 40-50% irregular structure, and 0-20% beta sheet. Two of the three tryptophan residues per protein molecule reside in hydrophobic regions, and the third is in contact with water. The distribution of the four tyrosine residues per protein molecule among hydrogen-bonding states is the same for capsid and virus but different for the disk. The latter could be a consequence of the presence of Cl- ions which may form hydrogen bonds with one or more p-hydroxyl groups of tyrosine residues.

Capsid↗

PubMed1978

Studies of virus structure by laser-Raman spectroscopy. Turnip yellow mosaic virus and capsids.

Laser-Raman spectroscopy of the turnip yellow mosaic virus (TYMV) and its capsid indicate the following features of the structure and assembly of the virion. The secondary structure of coat-protein molecules in TYMV is comprised of 9 +/- 5% alpha-helix, 43 +/- 6% beta-sheet, and 48 +/- 6% irregular conformation and is not altered by the removal of the RNA from the capsid. Introduction of as many as 200 chain scissions per RNA molecule also does not affect the overall secondary structure of the encapsulated RNA, which is 77 +/- 5% in the A-helix form. Tryptophan and cysteine residues of the coat protein appear to be in contact with the solvent, while only one of three tyrosines per coat protein is available for hydrogen bonding of its p-hydroxyl group with H2O molecules. Both cytosine and adenine residues of TYMV RNA are protonated in substantial numbers near pH 4.5, suggesting elevation of their respective pKa values within the virion. The Raman data are consistent with chemical evidence favoring interaction between protonated bases of RNA and amino acid side chains of coat protein in TYMV.

Capsid↗

PubMed1976