Biomedical subjects

K A Kaluniants

Publications and source records attributed to K A Kaluniants.

3 recordsLinked to original sources

PubMedDate not supplied

[Regularities in the synthesis of neutral protease from Bacillus subtilis].

Experiments were carried out to study the formation of extracellular protease by the suspension from Bacillus subtilis str. 103 washed cells selected at the growth stages characterized by the highest rate of the enzyme synthesis (after 12 and 35 hours). In relation to the protein and RNA synthesis as measured by 14C-valine and 14C-uracyl the cells of that age showed a high metabolic activity. The enzyme synthesis by the cells of the log-phase was inhibited completely with chloramphenicol and by the cells of the stationary phase--almost completely. The experiments demonstrated the protease synthesis insensitive to riphampycin for a long time. This may be indicative of a certain pool of long-lived mRNA responsible for the biosynthesis.

Bacillus subtilis↗

PubMedDate not supplied

[Enzyme complex celloconingine P10x].

The composition and properties of the enzyme preparation celloconingine P10x were studied. The preparation contained a number of citolytic enzymes and showed proteolytic and amylolytic activities. Optimal conditions for the enzyme action were found to be: for total citolytic and hemicellulase activities pH 5.2-6.2 and 45-55 degrees, for endo-beta-glucanase activity pH 4.2-5.2 and 58-62 degrees, for proteolytic activity pH 4.0-4.8 and 64-68 degrees, and for amylolytic activity pH 3.6-4.2 and 60-66 degrees C.

Glycoside Hydrolases↗

PubMedDate not supplied

[Effect of sources of carbon, nitrogen, and phosphorus on the synthesis of proteases from Bacillus subtilis cultures].

The paper gives data on the influence of different sources of carbon, nitrogen and phosphorus on the accumulation of biomass and synthesis of neutral protease by Bacillus subtilis str. 103. The highest proteolytic activity was obtained on the medium containing maltose or hydrolyzed starch as a carbon source, monopotassium phosphate as a phosphorus source, and ammonium sulphate as a nitrogen source. The enzyme activity increased upon an addition of albumin, peptone or casein. A study of the amino acid effect showed that methionine, isoleucine and valine stimulated the protease synthesis to the greatest extent.

Amino Acids↗