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Biomedical subjects

K A Langheinrich

Publications and source records attributed to K A Langheinrich.

At least 19 recordsLinked to original sources
PubMed2000

An evaluation of methods for the detection of spinal cord in product derived from advanced meat recovery systems.

This study investigated the usefulness of immunohistochemistry, histochemistry, and polarization microscopy in documenting the presence of spinal cord in ground product produced by advanced meat recovery systems (AMRS). A battery of diagnostic stains proved most helpful for definitive documentation of spinal cord in the AMRS product. Antibodies to neurofilament and glial fibrillary acidic proteins were useful for highlighting the presence of neural tissue in comminuted product but when used alone did not definitively differentiate central nervous system tissue from peripheral nerve. Polariscopy, trichrome staining, and immunostaining for synaptophysin were very useful for differentiating spinal cord from peripheral nerve. Spinal cord was found in 2 of 196 AMRS samples when only hematoxylin and eosin staining was used. In a later immunohistochemical subset of the survey study, spinal cord was found in 7 of 17 AMRS samples. More recently, spinal cord tissue has been found in 3 of 26 regulatory samples using hematoxylin and eosin stains combined with immunohistochemical techniques.

Animals↗
PubMed1996

A retrospective study of multicentric bovine renal cell tumors.

Tissues from twenty mature cows with primary renal cell tumors were submitted over an 11-year period because of gross lesions detected during routine slaughter and inspection. Tumors visualized grossly were multiple and bilateral in seven cattle, multiple within one kidney in four cattle, and solitary in nine cattle. The tumors were primarily cortical, yellow-orange to tan, proliferative, well circumscribed, and extended above the capsular surface of the kidney. Tumors were microscopically multiple even when grossly described as solitary lesions, except in one cow. Twelve tumors (60%) were microscopically multiple in one kidney, seven tumors (35%) were multiple and bilateral, and only one cow (5%) exhibited extrarenal metastasis. Tumors from nineteen cows were composed of eosinophilic granular epithelial cells; tumors from one cow were clear cell type. Each tumor contained several histologic patterns. Corpora amylacea, proteinaceous secretions, and hemosiderin were characteristic findings in bovine renal cell carcinoma. All 20 cows with renal cell tumors exhibited positive immunoreactivity to uromodulin (Tamm-Horsfall protein).

Animals↗
PubMed1994

Bovine pulmonary blastomas: histomorphologic description and immunohistochemistry.

Three adult female dairy cattle with pulmonary blastomas were evaluated. The gross lesions at postmortem were described as multiple white circumscribed masses throughout the lungs, with pleural involvement in one cow and lymph node metastasis in the other two cows. Histologically, the tumors contained a dual population of mesenchymal and epithelial neoplastic cells. Epithelial cells formed nests, tubules, and formations resembling bronchioles of normal lung. Mesenchymal cells were spindle shaped with oval nuclei and fibrillar eosinophilic cytoplasm, were large rounded cells with multiple round nuclei and granular eosinophilic cytoplasm, or appeared blast-like, with large hyperchromatic nuclei and amphophilic cytoplasm. The tumors varied greatly in appearance from one field to another within the same tumor. Epithelial cells stained positively with anticytokeratin antibodies. Some spindle-shaped mesenchymal cells exhibited smooth muscle cell differentiation with positive staining with anti-vimentin, anti-muscle-specific actin, and anti-smooth muscle actin antibodies, whereas other rounded mesenchymal cells expressed striated muscle cell differentiation with multiple nuclei and positivity to anti-neuron-specific enolase and anti-muscle-specific actin antibodies. The variable expression of the intermediate filaments and cytoplasmic enzymes indicates multiple pathways of differentiation in the pulmonary blastomas.

Animals↗
PubMed1993

Bovine myocardial epithelial inclusions.

Light microscopic, histochemical, immunohistochemical, and ultrastructural methods were used to examine myocardial epithelial masses in the hearts of ten cattle. The tissues consisted of paraffin-embedded or formalin-fixed samples from eight hearts that were being inspected in slaughter houses and from two hearts from calves that died of septicemia. The ages of the cattle ranged from 4 days to 12 years; the breeds were unspecified for all but one Hereford female and the two Holstein calves; and there were three males, four females, and three steers. The masses in these cases were compared with similar appearing lesions found in other animal species. The lesions in the bovine hearts were single to multiple, well circumscribed, found in the left ventricle wall, and composed of squamous to cuboidal epithelial cells that formed tubular, ductular, and acinar structures with lumens that were void or filled with amorphous protein globules. Electron microscopic examination revealed epithelial cells that had sparse apical microvilli, tight apical intercellular junctions, perinuclear bundles of filaments, and rare cilia. Almost half of the bovine epithelial masses (4/9) had occasional diastase-resistant periodic acid-Schiff-positive granules in their cytoplasm, and few had hyaluronidase-resistant alcian blue-positive granules (2/9) or colloidal iron-positive granules (1/9). All myocardial masses had abundant collagen surrounding the tubular and acinar structures, and 2/9 had elastin fibers as well. None of the myocardial masses had Churukian-Schenk or Fontana Masson's silver staining granules in epithelial cells. Immunohistochemically, all bovine myocardial tumors stained positively for cytokeratin (8/8), and occasional masses stained positively for vimentin (3/8) or carcinoembryonic antigen (3/8). None of the masses stained positively for desmin. The myocardial epithelial tumors most likely represent endodermal rests of tissue misplaced during organogenesis.

Animals↗
PubMed1992

Reticuloendotheliosis in a wild turkey (Meleagris gallopavo) from coastal Georgia.

An emaciated wild turkey (Meleagris gallopavo) exhibiting neurologic signs was found on Ossabaw Island, Chatham County, Georgia (USA) on 11 April 1989. The neurologic abnormalities observed included ataxia, drooping wings, head tremors, torticollis, and circling. At necropsy, discrete yellowish-white nodules, varying in size from 2 to 5 mm, were present in the spleen. White nodular lesions approximately 2 mm in diameter were observed beneath the mucosal surface of the distal esophagus. Histopathologic examination of the splenic nodules disclosed large numbers of primitive lymphoreticular cells with leptochromatic nuclei and abundant, slightly basophilic cytoplasms. The mitotic index in these cells was moderate to high. Similar neoplastic cells composed the masses observed in the esophagus. Multifocal, mild perivascular cuffing with mononuclear cells was found in the lumbar spinal cord, brain, and brain stem. Reticuloendotheliosis virus, subtype 3, was isolated from samples of the spleen and liver.

Animals↗
PubMedDate not supplied

Visceral granulomas in chickens infected with a filamentous bacteria.

Visceral granulomas containing gram-positive filamentous bacteria were diagnosed in eight chickens collected from processing plants over a period of 4 years. Formalin-fixed tissues were submitted from the chickens after slaughter, but no specimens suitable for bacterial culture were received. The bacteria lacked similar tinctorial, morphologic, and ultrastructural features of other gram-positive bacteria that have been identified in chickens. The bacteria were visible with hematoxylin and eosin and faintly visible with Gomori methenamine-silver, but negative with periodic acid-Schiff and Fite-Faraco acid-fast stains. Granulomas were found in the liver, spleen, and peritoneum.

Animals↗
PubMedDate not supplied

Proventricular hemangiosarcoma identified in a broiler at slaughter.

A discrete circumscribed red nodule present on the surface of the proventriculus of a 7-week-old broiler was diagnosed as a hemangiosarcoma. Histologically, discrete islands and dense sheets of anaplastic ovoid to spindle-shaped basophilic cells surrounded irregular vascular clefts and spaces. Mitotic figures were focally numerous, and extension into the tunica muscularis of the proventriculus was seen. Neoplastic islands were also present in the tunica mucosa and the adjacent mesentery.

Animals↗
PubMedDate not supplied

Neurofibrosarcoma and leiomyosarcoma in slaughterhouse broilers.

Two uncommon neoplastic lesions--a neurofibrosarcoma and a leiomyosarcoma--were found in 7-week-old slaughterhouse chickens. Grossly, the neoplasms both appeared as locally extensive, nodular lesions that displaced the mesentery and abdominal viscera. Histologically, these tumors were anaplastic and invasive into the surrounding tissues.

Abattoirs↗
PubMedDate not supplied

Polymelia in a broiler chicken.

A polymelus monster was observed in a 7-week-old slaughterhouse chicken. The supernumerary limbs were smaller than the normal appendages but contained an equal number of digits.

Animals↗
PubMedDate not supplied

Zygomycosis in the duck.

The fungal disease zygomycosis was diagnosed in the thoracic cavity of two 7-weeks-old ducks. In the first case, the diagnosis was based on examination of granulomas that were found to contain fungal hyphae characteristics of zygomycetes. The second case was diagnosed using similar criteria along with the subsequent culture of Mucor sp. from the lesion. To attain a definitive diagnosis of zygomycosis, the fungal hyphae found during histologic examination must be differentiated from Aspergillus sp. and Dactylaria gallopava.

Animals↗
PubMedDate not supplied

A retrospective immunohistochemical study of type II avian adenoviral infection in turkey, pheasant, and chicken tissues.

A retrospective study of avian cases submitted to the Michigan Animal Health Diagnostic Laboratory between 1986 and 1992 produced four pheasant, five turkey, and five chicken cases in which splenic tissue was positive for type II adenoviral antigen using an agar gel immunodiffusion assay. Tissue sections from these cases were evaluated microscopically using both routine hematoxylin and eosin (H&E) staining and an immunohistochemical staining technique for type II adenoviral antigen. Tissues from all four pheasant cases and four of five turkey cases, but none of the five chicken cases, stained positively by histochemistry. Five additional chicken cases, obtained from the U.S. Department of Agriculture Eastern Laboratory of the Food Safety Inspection Service, had splenic lesions of undetermined etiology; these were also evaluated. One of the five cases had splenic inclusions on H&E staining and had strong positive histochemical staining. The immunohistochemical staining technique was sufficiently sensitive for use on diagnostic tissues that have been routinely fixed in formalin and processed for histopathology, and it detected all three recognized type II adenoviruses.

Adenoviridae↗