Biomedical subjects
K A Leonard
Publications and source records attributed to K A Leonard.
Immunohistologic demonstration of myocardial proteins with applications.
The authors used antibodies specific for creatine kinase MB isoenzyme (CK-MB) and myosin light chain-1 (MLC-1) for immunohistologic staining. At appropriate dilutions of antibody, frozen sections of human heart muscle were positive for both CK-MB and MLC-1, whereas sections of human skeletal muscle were negative for both proteins. Staining for both CK-MB and MLC-1 also was demonstrated in an immature teratoma. Furthermore, staining was localized to the rhabdomyosarcomatous elements within the teratoma; other components of the tumor did not stain for CK-MB or MLC-1. Biopsies of skeletal muscle revealed that regenerative, but not intact normal or degenerating, fibers also contained CK-MB and MLC-1. Immunohistologic stains for CK-MB and MLC-1 may be useful as tumor markers and as markers for regenerative muscle fibers.
Secretion of creatine kinase MB isoenzyme by an immature teratoma with predominant rhabdomyosarcomatous elements.
A 37-year-old man with metastatic immature (malignant) teratoma with prominent rhabdomyosarcomatous elements had markedly increased activity of creatine kinase (EC 2.7.3.2) MB in serum. There was no electrocardiographic evidence of infarction or ischemia, and autopsy revealed no myocardial infarction, significant coronary atherosclerosis, myocarditis, or invasion of the heart by tumor. A high proportion of the creatine kinase activity in a homogenate of the tumor was attributable to the MB isoenzyme. Persistent increases of creatine kinase-MB and an unusually high MB isoenzyme activity, out of proportion to total creatine kinase activity, may indicate a nonmyocardial origin of this isoenzyme.
Accelerated induction to two-kidney hypertension in rats and renin-angiotensin sensitivity.
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Firearm deaths in Canadian adolescents and young adults.
This article describes the problem of firearm deaths among Canadians aged 15-24 years. It is based on data obtained from Statistics Canada, the Metropolitan Toronto Police Department, and the Canadian Department of Justice. Firearms are the third leading cause of death in this age group, accounting for 276 deaths in 1990, after motor vehicle accidents (997) and non-firearm suicides (358). Some 23% of Canadian homes contain a firearm; the average number of firearms per home is 2.67. Medical and public health professionals are urged to work toward prevention by educating patients and families about the risks of a firearm in the home and by supporting legislation to decrease the availability of firearms to young people.