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Biomedical subjects

K Adetugbo

Publications and source records attributed to K Adetugbo.

16 recordsLinked to original sources
PubMed2000

How well are randomized controlled trials reported in the dermatology literature?

OBJECTIVE: To assess the methodological quality of the design and reporting of randomized controlled trials published in one major dermatology specialty journal. DESIGN AND DATA SOURCES: In a survey of all published parallel group randomized controlled trials, we found 73 reports with allocation described as randomized from all issues of Clinical and Experimental Dermatology from its inception in 1976 through 1997. MAIN OUTCOME MEASURES: Direct and indirect measures of the adequacy of randomization, trial sample size, baseline comparisons, and intention-to-treat analysis. RESULTS: Hand searching identified 73 randomized controlled trials, but only 31 of these were found by searching MEDLINE for the publication type clinical trials. Of the 73 randomized controlled trials, 68 contained sufficient information to include in the analysis. Only 1 study (1%) reported the method of random sequence generation, and only 5 studies (7%) reported adequate concealment of allocation. Among 38 trials that used simple randomization, the sample sizes in the comparison groups were identical in 22 occasions, raising the possibility that simple randomization might not have been adequately generated or concealed. Most trials (88%) excluded some randomized participants from their analysis. The median sample size was 23 per trial. Only 1 trial reported sample size and statistical power considerations and had an a priori main hypothesis. CONCLUSIONS: Hand searching is important for locating all relevant trials. There is the need for higher methodological quality in clinical trial reporting in dermatology journals. The adoption of the CONSORT (Consolidated Standards of Reporting Trials) statement and checklist for the reporting of trials should enhance the validity of and strengthen the evidence from clinical trials reports.

Data Collection↗
PubMed1998

The Cochrane Skin Group. Preparing, maintaining, and disseminating systematic reviews of clinical interventions in dermatology.

In 1979, Prof Archie Cochrane challenged the medical profession to produce a critical summary of randomized controlled clinical trials according to specialty, which should be updated periodically. The Cochrane Collaboration, an international voluntary group of reviewers and researchers from a range of professional backgrounds dedicated to producing systematic reviews, was established in 1992 in response to Cochrane's challenge. Systematic reviews produced by the Cochrane Collaboration start with individuals who formulate questions that are important to the care of patients. Every effort is then made to locate published and unpublished evidence to answer the question, and explicit criteria are used to select studies for inclusion in the review and to assess their quality. If appropriate, meta-analysis is used to combine results from several smaller studies to produce an overall result. Reviews are published in the Cochrane Library, an electronic publication (CD-ROM or diskette form), and the reviews are updated quarterly. In December 1997, a Cochrane Skin Group was registered with the Cochrane Collaboration to prepare, maintain, and disseminate reviews on the effects of health care for people with dermatological conditions. Currently, 25 titles and 9 review protocols have been registered with the Cochrane Skin Group, and the first set of dermatological reviews will be available before the end of 1999. The Cochrane Skin Group aims to become the best source of unbiased external evidence for summarizing the effects of dermatological care.

Dermatology↗
PubMed1997

Effect of early complementary feeding on nutritional status in term infants in rural Nigeria.

Breastfeeding is common in developing countries, but exclusive breastfeeding is rare, and complementary foods are introduced at an early age. The objective of the present study is to determine the effect of early complementary feeding on the nutritional status of infants. Weight-for-age (WA) indices have been determined for 82 infants 3 to 4 months old participating in a breastfeeding study. They included 42 that started complementary feeding early (before 2 months) and 40 that started later. Weight-for-age indices were significantly lower for the early group than for the later group (t = 3.00, p = 0.004). The prevalence of underweight (WA SD scores below -2.0) was 7.5% in the later complementary feeding group and 28.6% in the early group (chi(2) = 4.76, p = 0.0292). Severe underweight (WA SD scores below -3.0) prevalences were 0% and 14.3% respectively (p = 0.0259). Thus, poorer nutritional status was significantly associated with earlier complementary feeding. The results suggest that exclusive breastfeeding, (together with promotion of weaning education and growth monitoring) should be vigorously promoted in these rural communities.

Body Weight↗
PubMed1997

Breast-feeding promotion in a diarrhoea programme in rural communities.

Breast-feeding promotion is an important intervention for the control of infant diarrhoea. This study assesses the impact of a breast-feeding counselling programme on the prevalence of exclusive breast feeding in rural communities in Nigeria. Mothers attending primary care facilities because their infants had acute diarrhoea were randomised into two groups. The study group (n = 82) received individual, focused breast-feeding counselling, while the controls (n = 79) had routine advice for diarrhoea. Both groups were monitored and followed with home visits to determine infant-feeding practices. The results showed marked increases in exclusive breast-feeding prevalence for the intervention group at day 7 (49% vs. 6% control; p < 0.0001) and day 21 (46% vs. 8%; p < 0.0001). Moreover, diarrhoea re-occurrence at day 21 was less in the intervention group (12%) than controls (18%). It is concluded that focused breast-feeding counselling can increase exclusive breast feeding and reduce the prevalence of diarrhoea in rural communities.

Adolescent↗
PubMed1997

Breastfeeding training improves health worker performance in rural Nigeria.

The training of health workers in breastfeeding and lactation management is to enable them make correct breastfeeding recommendations to mothers. This study aims to provide answers to two research questions: what components of breastfeeding training are easily achieved with extension health workers, and what health worker variables affect these outcomes. Multivariable analysis of the outcomes of a controlled breastfeeding training programme for community health workers (CHW) in rural communities of Osun State, Nigeria, was performed by logistic regression. The results show that the training was the most powerful predictor of correct CHW recommendations on breastfeeding (OR = 60.25, p-0.0000), and of 'perfect' breastfeeding knowledge (OR = 192.49, p = 0.0000). Younger CHWs (in the age bracket 20 to 29 years) were significantly more likely to make correct recommendations on exclusive breastfeeding (OR = 3.02, p = 0.0304). Other CHW variables such as sex, experience, job status, and marital status did not make consistently significant independent contributions to the outcomes. The results suggest that breastfeeding education can enhance CHW professional recommendations on breastfeeding and should be extended to all categories of health workers.

Adult↗
PubMed1978

Evolution of immunoglobulin subclasses. Primary structure of a murine myeloma gamma1 chain.

The amino acid sequences of the first six CNBr fragments (303 residues) of MOPC 21 heavy chain have been determined, using conventional techniques. This completes the determination of the covalent structure of MOPC 21 immunoglobulin (IgG1, K). MOPC 21 gamma1 chain is a unique sequence of 440 amino acid residues. The variable region is homologous with human VHIII sequences. A comparison of the constant region with homologous sequences shows that mouse gamma1 and gamma2 proteins differ much more than the gamma subclasses in man and in some other species and supports earlier suggestions that mouse gamma1 and the four human gamma subclasses have a common ancestor different from the mouse gamma2 ancestor. Unlike some other subclasses, but like those in the guinea pig, mouse gamma1 and gamma2 genes have diverged before speciation. An expansion-contraction mechanism is proposed for the evolution of gamma genes.

Amino Acid Sequence↗
PubMed1978

Spontaneous somatic mutations. Structural studies on mutant immunoglobulins.

The precise alterations in the protein amino acid sequences of the immunoglobulin heavy chains of spontaneously arisen MOPC 21 mutant clones IF2 and IF1 have been determined. All the cyanogen bromide fra-ments of both heavy chains have been isolated and compared to the wild type CNBr fragments. For IF2, there is an internal deletion, from the wild type sequence, of 96 amino acids, from residues 121 to 215 inclusive. Moreover, in IF2, there are no disulfide bonds formed between heavy and light chains, presumably because of the deletion of the CH1 pseudosubunit. There are no other alterations in its covalent structure. For IF1, there is a deletion of the COOH-terminal 83 amino acids (residues 358 to 440, inclusive). Although IF1 heavy chain behaves on dodecyl sulfate-polyacryl-amide gels as if it were only 10 to 15 residues shorter than wild type, no other amino acid sequence differences from wild type are found. IF1 arose most likely by a nonsense mutation of a serine codon. For IF2, whose deletion is like that seen in some human heavy chain disease proteins, the most likely explanation is an error of recombination. The structure of IF2 suggests that the heavy chain variable region ends at a position homologous to residue 120 of the MOPC 21 heavy chain.

Amino Acids↗
PubMed1978

Expression of antibody genes in tissue culture: structural mutants and hybrid cells.

Detailed information on the nature and frequency of somatic mutations has been derived from studies of the clonal diversification of the myeloma MOPC 21 in tissue culture. A screening procedure is described that permitted the isolation of four spontaneous mutations at the gamma1 structural gene locus. These originate from four mutation events. Two seem to be point mutations: a "nonsense" and a "mis-sense." Of the other two, one is a frameshift leading to mistranslation and early termination, the other a large deletion due to perhaps an intrachromosomal translocation or a mitotic recombination. Fusion between myeloma-producing cells has shown that variable and constant region genes cannot be scrambled. Differentiation from stem to plasma cells seems to involve changes in the primary sequence of the DNA. Fusion between myeloma cells and spleen cells from immunized animals is a satisfactory method for the derivation of permanent tissue culture lines producing specific antibody. The hybrids express the myeloma as well as the specific antibody light and heavy chains. By subcloning and selection, one can derive lines that selectively lose individual chains. Lines that no longer express the myeloma components can thus be derived. The use of appropriate defective variants of the myeloma parental line is another way of avoiding the presence of the myeloma components.

Animals↗
PubMed1977

Molecular analysis of spontaneous somatic mutants.

Eukaryotic structural gene mutations occurring spontaneously in a mouse myeloma cell line offer the opportunity to study somatic mutation in animal cells at the molecular level. Studies on the myeloma protein and on mRNA have enabled us to characterise four such mutants representing four different mutation mechanisms. The results may have some bearing on the origin of antibody diversity.

Amino Acid Sequence↗
PubMed1975

Mouse immunoglobulin subclasses: cyanogen bromide fragments and partial sequence of a gamma1 chain.

The purification and characterisation of all the cyanogen bromide fragments of MOPC 21 heavy (gamma1) chain is described. The ten BrCN fragments account for the whole chain. Four of these fragments have been used to establish the sequence of the C-terminal stretch (138 residues) that includes the entire CH3 and a part of the CH2 homology regions. A comparison of this sequence with homologous sequences is presented. Mouse gamma1 and gamma2 proteins differ much more than the gamma subclasses in humans and in other species. The comparison further suggests that the four human gamma subclasses and mouse gamma1 have a common ancestor which differs from the mouse gamma2 ancestor. Unlike other subclasses, mouse gamma1 and gamma2 genes have diverged before speciation.

Amino Acid Sequence↗