Modified EA staining solution using fast green in the Papanicolaou method.
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Eosine Yellowish-(YS)↗
Biomedical subjects
K Akura
Publications and source records attributed to K Akura.
[Evaluation of trypsin pretreatment in Giemsa's staining].
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Chromosomes↗
Use of immunocytochemistry on urinary sediments for the rapid identification of human polyomavirus infection. A case report.
The application of immunocytochemistry in urinary cytology for the identification of a human polyomavirus infection is described. The Papanicolaoustained slides of voided urine specimens of a 26-year-old man undergoing steroid therapy showed many inclusion-bearing epithelial cells. After subsequent destaining of the same slides, the presence of human papovavirus antigen in the nuclei of infected exfoliated cells was demonstrated by immunocytochemical staining with simian virus 40 antiserum and peroxidase. Papovaviruses were also detected by electron microscopic study of the smears, confirming the diagnosis of a human polyomavirus infection. The use of immunoperoxidase studies proved to be advantageous for the rapid cytodiagnosis of human polyomavirus infection in the urinary specimens in this case; such studies may be of particular value in equivocal cases to prove or disprove the viral nature of morphologic changes observed in routine preparations.
Adult↗