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K Anil

Publications and source records attributed to K Anil.

6 recordsLinked to original sources
PubMed1997

Effect of n-3 fatty acids on VLDL production by hepatocytes is mediated through prostaglandins.

The mechanism of the hypolipidemic effect of n-3 fatty acids was studied using isolated rat hepatocytes maintained in culture. EPA and DHA caused a significant reduction in the incorporation of 3[H]-leucine into apoB associated with the VLDL produced by hepatocytes in culture when compared to that in presence of palmitic acid. Presence of indomethacin, an inhibitor of cyclo-oxygenase reversed the effect of EPA on VLDL synthesis while diethyl carbamazine an inhibitor of lipoxygenase did not show any effect suggesting that the effect of EPA may be mediated through prostaglandins. This was further tested by invivo experiments where animals were fed fish oil containing diet with and without aspirin, which inhibits formation of prostaglandins. The incorporation of 3[H]-leucine into apo B and 14[C]-acetate into cholesterol of VLDL produced by hepatocytes from aspirin treated animals were significantly high. The reversal of the effect of n-3 fatty acids by agents which inhibit the formation of prostaglandin suggests that the n-3 fatty acids may exert their effect on VLDL production by liver cells through prostaglandins.

Animals↗
PubMed1995

Blocking of alcohol-induced effects on synthesis and secretion of apo B containing lipoproteins by substituting sardine oil in the diet.

The effect of substitution of fish oil in the diet on the alcohol-induced changes in the metabolism of very low density lipoprotein (VLDL) by primary cultures of rat hepatocytes has been studied. Rats fed groundnut oil diet or sardine oil diet were given alcohol (3 g/kg) for 4 weeks. Substitution of fish oil for groundnut oil in the diet blocked the hypertriglyceridemia and hyperlipoproteinemia caused by alcohol. Enhanced incorporation of [3H]leucine into apo B secreted into the medium and [14C]acetate into lipids associated with secreted VLDL indicated an increased rate of synthesis of apo B containing lipoproteins by hepatocytes from livers of rats receiving alcohol. Fish oil in the diet reduced incorporation of [3H]leucine into apo B and that of [14C]acetate into lipids indicating a lower rate of synthesis of apo B containing lipoproteins. Pulse chase experiments confirmed the above observation. Thus it is suggested that fish oil in the diet prevents hyperlipoproteinemia caused by alcohol possibly by reducing the synthesis and secretion of VLDL by liver.

Animals↗
PubMed1992

Metabolism of very low density lipoproteins--effect of sardine oil.

The effect of feeding fish oil on the metabolism of lipoproteins was studied in rats. Rats were fed diet containing 10% sardine or groundnut oil for 6 weeks. There was a significant decrease in the total cholesterol, phospholipids and triglycerides as well as the amount of the lipids associated with VLDL and LDL in serum in fish oil-fed rats. The synthesis and secretion of lipoproteins particularly apoB containing lipoproteins by primary cultures of hepatocytes from these rats were studied by 14(C)-acetate or 3(H)-leucine labelling. Primary cultures of hepatocytes derived from sardine oil-fed rats showed reduced incorporation of 3(H)-leucine into apoB containing lipoproteins secreted into the medium when compared to those fed groundnut oil, indicating a decreased synthesis and secretion of apoB. This was further confirmed by significantly lower incorporation of 14(C)-radioactivity into total and individual lipids of VLDL secreted into the medium, as well as that associated with different lipids in cell layer. The activity of lipoprotein lipase in adipose tissue and aorta was significantly higher in rats fed sardine oil which may cause an increased clearance of triglyceride-rich lipoproteins from circulation. These results indicate that the fish oil exerts hypolipidemic effect particularly by decreasing the synthesis and secretion of VLDL by liver and possibly by an increased clearance of triglyceride-rich lipoproteins from circulation.

Administration, Oral↗
PubMedDate not supplied

Effect of feeding sardine oil on synthesis and secretion of apolipoprotein B-containing lipoproteins by rabbit hepatocytes.

The effect of feeding sardine oil on the metabolism of lipoprotein was studied in rabbits. Rabbits were fed a diet containing 10% sardine oil or groundnut oil for 6 wk. A significant decrease in serum cholesterol and triglycerides was observed in rabbits receiving sardine oil. Radiolabeling studies with [3H]leucine and [14C]acetate on the synthesis and secretion of apolipoprotein B (apoB)-containing lipoproteins by primary cultures of rabbit hepatocytes were carried out. Significantly lower incorporation of [3H]leucine into apoB secreted into the medium by hepatocytes derived from rabbits fed sardine oil indicates a reduced synthesis and secretion of apoB-containing lipoproteins. This was further confirmed by a decreased incorporation of [14C]acetate into cholesterol and cholesteryl ester associated with lipoprotein floating at the very-low-density lipoprotein density range. Radioactivity incorporated into cholesterol associated with the cell layer was also reduced. These results indicate that sardine oil exerts a hypolipidemic action in rabbits mainly by decreasing the synthesis and secretion of apoB-containing lipoproteins by liver.

Acetates↗
PubMedDate not supplied

Haptoglobin in rheumatic fever.

Serum haptoglobin levels were studied in rheumatic fever and in established rheumatic heart disease to find whether rheumatic activity influenced serum haptoglobin levels. The study was carried out on 90 subjects of paediatric age group, comprising 30 children suffering from acute rheumatic fever (Group I) and Group II of 30 children of established rheumatic heart disease (not having rheumatic activity). They were matched with group III of 30 normal children of the same age as controls. Levels of ASO titre, C-reactive protein, serum haptoglobin and ESR were estimated in all cases of study and control groups. Estimations of serum haptoglobin levels and ESR were repeated in all children of rheumatic fever after 2 weeks. Haptoglobin levels were estimated by electrophoresis on a cellulose acetate paper. In healthy children (Group III) mean serum haptoglobin was 92.2 +/- 19.77 mg%. This was significantly lower when compared with acute rheumatic fever group before treatment (606.58 +/- 171.04 mg%) and rheumatic heart disease group (120.8 +/- 26.03 mg%).

Child↗