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K Arisue

Publications and source records attributed to K Arisue.

At least 19 recordsLinked to original sources
PubMed1998

Clinical study of refractory apical periodontitis treated by apicectomy. Part 1. Root canal morphology of resected apex.

The morphology of the root apex was analysed by observation of the anatomy of specimens obtained by apicoectomy in cases of refractory apical periodontitis that did not respond to nonsurgical root canal treatment. Apical ramifications were present in 19 (70%) of the roots, while one were found in the remaining eight (3%) roots. This frequency is far higher than that reported by other investigators, suggesting that there is a close relationship between the anatomical complexity of the root canal and the occurrence of refractory apical periodontitis.

Apicoectomy↗
PubMed1995

Assay of magnesium in serum and urine with use of only one enzyme, isocitrate dehydrogenase (NADP+).

We report a method for assaying magnesium in serum and urine involving only one enzyme, isocitrate dehydrogenase (NADP+)(EC 1.1.1.42), which requires magnesium ion for activity. The enzymatic reduction of NADP+ by isocitrate increases in rate linearly up to at least 20 mmol/L magnesium in the presence of appropriate concentrations of the two metal-chelating reagents, EDTA and glycol ether diamine-N,N,N',N'-tetraacetate. Within-run (n = 20) CVs and day-to-day (n = 10) CVs for sera are < or = 1.5% and < or = 2.6%, respectively. Analytical recovery of magnesium in sera averages 96-100%. This method is not affected by bilirubin, hemoglobin, or lipemia. The method (y) gives the following results correlating with atomic absorption spectrophotometry (x): y = 1.03x + 0.06 mmol/L (n = 62, r = 0.995, Sylx = 0.03) for sera, and y = 1.03x - 0.10 mmol/L (n = 62, r = 0.989, Sylx = 0.19) for urines; with the calmagite method (x): y = 0.99x + 0.04 mmol/L (n = 62, r = 0.991, Sylx = 0.03) for sera, and y = 0.98x + 0.03 mmol/L (n = 62, r = 0.999, Sylx = 0.02) for urines.

Autoanalysis↗
PubMed1994

[Changes in serum lipid and lipoproteins in alloxan-diabetic rats--studies for one year].

Alloxan-diabetic rats were kept on an ordinary or 0.25% cholesterol supplemented diet for up to 12 months, and changes in the serum lipid and lipoprotein levels were examined. The diabetic rats showed IRI levels of 3-5 IU/ml, which were 1/5-1/10 that of the normal rats, and about 10 times higher serum glucose levels than the normal rats. The diabetic rats consumed 1.5-2 fold amounts of diet but their body weights were almost constant. The diabetic rats showed marked hyperlipidemia, especially in the cholesterol and triglyceride levels. The phospholipid level also increased but for a lesser extent and the c/p ratio increased. The serum lipoprotein levels increased in the diabetic rats, 5-10 times in the ordinary diet fed groups and 10-20 times in the cholesterol diet fed groups. Chylomicron, VLDL, IDL and LDL increased but HDL decreased. These changes were more significant in the diabetic rats fed the cholesterol diet. The triglyceride levels in the lipoprotein fractions increased in the diabetic rats, but the increase in cholesterol was more significant and the relative composition ratios of triglyceride decreased. The composition ratios of phospholipids, however, remained almost constant in all the lipoprotein fractions. The increase in IDL, especially cholesterol in the IDL fraction, was remarkable in the diabetic rats. The responsiveness of the diabetic rat VLDL to lipoprotein lipase (release of free fatty acids) was less than a half of that of the normal rat VLDL. In conclusion, the diabetic rats showed an increase in triglyceride but more significant increase in cholesterol in the lipoprotein fractions. These changes in serum lipid and lipoprotein levels and compositions appeared as early as 3 months after the alloxan injection and lasted for 12 months without any progress in the disturbance.

Alloxan↗
PubMed1986

Automatic analysis of serum lactate dehydrogenase isoenzymes by high-performance ion-exchange chromatography.

The repetitive analysis of serum lactate dehydrogenase (LDH) isoenzymes has been performed on a weak anion exchanger (TSKgel DEAE-5PW), which was developed by introducing diethylaminoethyl groups into TSKgel G5000PW (10 microns particle diameter)--a hydrophilic polymer-based material of large pore size--for high-performance gel chromatography. By use of this anion exchanger, a high-pH (greater than 8.0) solvent could be used and the albumin peak was completely separate from the LDH isoenzyme peaks. After 10 successive analyses with an autosampler, the coefficient of variation of the LDH isoenzyme elution times was less than or equal to 0.90%, and the coefficient of variation for peak areas was less than or equal to 3.85%. After 40 successive analyses, resolution between isoenzymes was generally greater than 1.25. This column can be used for more than 300 intermittent injections of human serum.

Autoanalysis↗
PubMed1984

Automated enzymatic determination of serum and urine creatine using the Abbott ABA-200.

A new enzymatic method is described for the determination of creatine in serum and urine with Abbott ABA-200. The measurement is accomplished by transforming creatine to formic acid in a reaction catalyzed by creatinase (creatine amidinohydrolase), sarcosine oxidase and formaldehyde dehydrogenase (see Figure 1). The assay takes less than 20 minutes. The standard response is linear for creatine concentrations up to 10 mg/dL (serum) and 80 mg/dL (urine). The coefficients of variation at 0.69 mg/dL (serum) and 4.93 mg/dL (urine) for within-day determination were less than 4% and, for between-day determination, were less than 5%. Results obtained by this procedure on one hundred serum and urine samples conformed well with a manual enzymatic method and the Folin method. The method is useful for the automated measurement of creatine in serum and urine.

Aldehyde Oxidoreductases↗
PubMed1983

Effects of sodium ursodeoxycholate, hyodeoxycholate and dehydrocholate on cholesterol and bile acid metabolism in rats.

Effects of sodium ursodeoxycholate, hyodeoxycholate and dehydrocholate on serum and liver cholesterol levels, bile flow, biliary cholesterol, phospholipid and bile acid secretions, and fecal sterol and bile acid excretions were examined with Wistar strain male rats fed ordinary and 2% cholesterol supplemented diets. Dehydrocholate increased the liver cholesterol level, bile flow and biliary lipid secretion, but ursodeoxycholate and hyodeoxycholate did not. The serum cholesterol level was not changed by the treatments. Ursodeoxycholate and hyodeoxycholate increased their own secretion into the bile and decreased cholic acid secretion, while dehydrocholate increased deoxycholic acid and oxo bile acid secretion. Ursodeoxycholate increased but dehydrocholate decreased the fecal sterol excretion, and hyodeoxycholate caused no change. Dehydrocholate decreased the fecal coprostanol level. The total amounts of the fecal bile acids were similar in all the treated groups, but ursodeoxycholate increased lithocholic acid, alpha, beta- and omega-muricholic acids and ursodeoxycholic acid; hyodeoxycholate increased hyodeoxycholic acid, 3 alpha, 7 beta, 12 alpha-trihydroxy-5 beta-cholanoic acid and oxo bile acids; and dehydrocholate increased deoxycholic acid, cholic acid, omega-muricholic acid and oxo bile acids and decreased hyodeoxycholic acid. These data suggested that ursodeoxycholate was transformed into lithocholic and muricholic acids, and dehydrocholate into cholic and deoxycholic acids during the enterohepatic circulation, but hyodeoxycholate showed almost no change. Ursodeoxycholate and hyodeoxycholate caused neither accumulation of cholesterol in tissues nor increase in bile flow and biliary lipid secretion as well as chenodeoxycholate did. The biological effect of dehydrocholate was similar to that of cholate, and this was partially due to its conversion into cholic acid and deoxycholic acid.

Animals↗
PubMed1983

Semi-automated continuous-flow enzyme immunoassay for antiepileptic drugs in serum.

We have developed a semi-automated method for measuring five kinds of antiepileptic drugs in serum by successfully adapting commercial competitive-binding enzyme immunoassay kits (MARKIT; Dainippon) for use with a continuous-flow analyzer (Technicon AutoAnalyzer II equipped with a dialyzer). The free enzyme-labeled drug is automatically separated by a microfilter from the competitive immunoreaction mixture between labeled and unlabeled drug for anti-drug immunoglobulin coupled to bacterial cell walls. The concentrations of the antiepileptic drugs in serum samples can be determined by automated measurement of enzyme activity of the enzyme-labeled drugs. Results of the semi-automated method correlated well with those obtained by manual enzyme immunoassay, gas-liquid chromatography, and "high-pressure" liquid chromatography. The correlation coefficients were all greater than 0.95, showing the practicality of this method for therapeutic monitoring of antiepileptic drugs.

Anticonvulsants↗
PubMed1982

A new enzymatic method to determine creatine.

A new enzymatic method is described for the determination of creatine in serum and urine using creatine amidinohydrolase (EC 3.5.3.3), sarcosine oxidase (EC 1.5.99.1) and formaldehyde dehydrogenase (EC 1.2.1.1). The principle of the method is as follows. Creatine is degraded to sarcosine and urea, and the sarcosine formed is measured with sarcosine oxidase in the presence of formaldehyde dehydrogenase and NAD+. The NADH + H+ produced is measured at 340 nm. Creatine concentration can be calculated directly from the absorptivity of NADH + H+ generated in the reaction or from creatine standard solutions. The assay takes less than 20 min. The standard curve is linear up to 50 mg creatine/1 (serum) and 800 mg creatine/1 (urine). Fifty random samples were assayed by this method (y) and simultaneously by the Folin method (x). The correlation coefficients were 0.995 for serum samples, 0.994 for urine samples, and the regression equations were y = 0.979x - 0.01 (serum) and y = 0.978x - 0.01 (urine).

Aldehyde Oxidoreductases↗
PubMed1982

Assays of serum lipase by the "BALB-DTNB method" mechanized for use with discrete and continuous-flow analyzers.

We successfully adapted the dimercaprol (BAL) tributyrate-5,5'-dithiobis(2-nitrobenzoic acid) method (J. Biochem. 81: 361, 1977) for assay of lipase in human serum to a discrete analyzer (the TBA 880) (I) or a continuous-flow analyzer (AutoAnalyzer, Type II) (II). In both, BAL-tributyrate is used as substrate, in combination with serum esterase inhibitors and a chromogenic reagent for the SH group of the liberated BAL. Serum lipase activities of patients with pancreatic diseases, measured at 90 or 40 samples per hour by I or II, respectively, correlated well with those measured by the corresponding manual method or by Kaplan's radioassay (Anal. Biochem. 33: 213, 1970). The correlation coefficients were all greater than 0.95, and the coefficients of variation were less than 8%, showing the practical usefulness of these procedures.

Autoanalysis↗
PubMed1981

Continuous-flow enzymic determination of creatine in urine.

A new enzymic method is described for the determination of creatine in urine by continuous-flow analysis. The measurement is accomplished by transforming creatine to formaldehyde in reactions catalyzed by creatinase (creatine amidinohydrolase) and sarcosine dehydrogenase. The formaldehyde is reacted with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole to form a purple product, which is measured colorimetrically. The method gives a linear standard curve for creatine concentrations up to 100 mg/L. Precision and analytical recovery are excellent, and results correlate well with those by the more-difficult Folin method, which currently is most often used for this analysis.

Autoanalysis↗
PubMed1980

Enzyme immunoassay of hepatitis B surface antigen with use of the "Hepanostika" test kit and a new photometer, "Photo-Elisa 1".

We describe an enzyme immunoassay for hepatitis B surface antigen, with use of a Hepanostika test kit and "Photo-Elisa 1," a photometer capable of measuring small quantities of test material. Because the length of the optical path in the "Photo-Elisa 1" is only 1.2 mm, volumes as small as 15 micro L can be can be measured. Accuracy is enhanced through cancellation of background absorbance by measurement at two wavelengths. Results obtained with the kit compare well with those by radioimmunoassay. By substituting photometric reading for visual reading, Hepanostika provides a more nearly accurate screening test.

Enzyme-Linked Immunosorbent Assay↗