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Biomedical subjects

K Asada

Publications and source records attributed to K Asada.

At least 127 records · Page 7Linked to original sources
PubMed1990

The polymerase chain reaction for Mycoplasma pulmonis.

In vitro DNA amplification by polymerase chain reaction was examined to detect Mycoplasma pulmonis. A pair of synthetic oligonucleotide primers was constructed, and used to amplify a unique sequence of M. pulmonis DNA. Amplified products were detected by agarose gel electrophoresis and verified by blot hybridization with a synthetic oligonucleotide probe. This system detected cellular DNA of M. pulmonis but not M. arthritidis or M. neurolyticum, and thus appears to be useful for M. pulmonis diagnosis.

Base Sequence↗
PubMed1990

Rapid and reliable protocol for direct sequencing of material amplified by the polymerase chain reaction.

A simple and reliable method is described for direct sequencing of material generated by the polymerase chain reaction. The protocol is based on the purification of the amplified double-stranded product by polyethylene glycol precipitation, annealing of primer with template by a "snap-cooling" procedure and sequencing by the dideoxy chain termination method with the use of Klenow fragment or Taq polymerase. The limit of the size of PCR products that can be sequenced is also discussed.

Base Sequence↗
PubMed1989

The primary structure of porcine liver acylamino acid-releasing enzyme deduced from cDNA sequences.

Two overlapping cloned cDNAs encoding the entire amino acid sequences of the subunits of acylamino acid-releasing enzyme (AARE) [EC 3.4.19.1] have been isolated from porcine liver cDNA lambda gt10 cDNA libraries and sequenced. Sequence analyses of the cDNA and several Achromobacter protease I-digested peptides of the purified protein revealed that porcine liver AARE consists of four identical subunits, and each comprising a single chain of 732 amino acids with acetylmethionine at the N-terminus.

Animals↗
PubMed1989

cDNA cloning of human calpastatin: sequence homology among human, pig, and rabbit calpastatins.

cDNA of human calpastatin, an inhibitor protein specific for calpain (EC 3.4.22.17; Ca2(+)-dependent cysteine proteinase) was isolated by screening of a library prepared from human liver mRNA with pig calpastatin cDNA fragment as a probe. The primary structure of human calpastatin was deduced from the nucleotide sequence of the cDNA and compared with that of pig and rabbit calpastatins already reported. Human calpastatin consisted of 673 amino acid residues and had 78% and 77% identity to pig or rabbit calpastatins, respectively. Human calpastatin had a domain structure with four internally repetitive sequences and one N-terminal non-homologous sequence like the other calpastatins. Human calpastatin had two deletions, 22 and 13 residues long in domain L and domain 1, respectively, compared to pig or rabbit calpastatins.

Amino Acid Sequence↗
PubMed1989

[Case of sialolithiasis with severe fatty change of the submandibular salivary gland].

We encountered a 57-year-old female with sialolithiasis associated with severe fatty change in the submandibular salivary gland. The submandibular salivary gland was removed under general anesthesia, and the postoperative course was uneventful. Histopathological changes observed in submandibular salivary glands with sialoliths are mainly inflammatory cell infiltration or connective tissue infiltration. On the other hand, increase in fat cells with age has been reported, but these age-related fatty changes are not severe. It was considered that the cause of the fatty change in this case was related to anoxia, which increased with long-term stasis of the duct system that was brought on by the development of the sialolith.

Adipose Tissue↗
PubMed1989

[Arthrography of recurrent dislocation of the TMJ--with special reference to the movement of the articular disk].

To investigate the movement of the articular disk in recurrent luxations, 4 temporomandibular joints in three patients were studied with the arthrotomographs. All the superior articular cavities were extended anteriorly. On the mouth open in two patients with recurrent subluxation, the mandibular head accompanied with the articular disk located very anterior position from the articular tubercle. At this time, the retro-discal pad were stretched, but the relation between mandibular head and articular disk were normal findings: the mandibular head located on the central thin part of the articular disk. One of them showed anterior disk displacement with reduction. The other patient showed the bone cavity at the anterior slope of the articular tubercle. On the mouth open, the mandibular head and posterior band fitted that cavity. The other hand, remaining one patient was recurrent luxation on the left side. On the mouth open, the relation between mandibular head and articular disk were abnormal findings: The mandibular head located very anterior position, over the articular tubercle and anterior band of the disk. Therefore, the inferior articular cavity was extended, but the retro-discal pad was not stretched. The anterior slope of the articular tubercle was steep. The other side TMJ of this patient was recurrent subluxation and anterior disk displacement with reduction. On the mouth open, it was showed the hypermobility of mandibular head. But the relation between mandibular head and articular disk were normal findings. As mentioned above, in luxation and subluxation, it was made obvious that there were tow types about the relation between mandibular head and articular disk on the mouth open: one type was that mandibular head located anteriorly to the anterior band of the disk, the other was under the disk.

Arthrography↗
PubMed1988

COOH-terminal residues of D1 and the 44 kDa CPa-2 at spinach photosystem II core complex.

The COOH-termini of the 32 kDa D1 and 44 kDa CPa-2 were determined by protein sequencing of peptides from trypsinized photosystem II core complexes. COOH-terminal fragments were isolated by affinity chromatography using anhydrotrypsin-agarose. One peptide had a sequence corresponding to the segment from Asn at position 335 to Ala at position 344 of the sequence deduced from the psbA gene coding for D1. Nine amino acids may be cleaved from the COOH-terminus of pre-D1 during maturation. In contrast, CPa-2 was not modified at its COOH-terminus.

Amino Acid Sequence↗
PubMed1988

Augmentation of coronary bypass graft flow induced by dipyridamole and its relation to bypass graft patency.

To evaluate the effect of dipyridamole on coronary bypass graft flow, 10 mg of dipyridamole was injected intravenously, during the measurement of graft flow, at the time of surgery. Its concentration in serum was measured and compared with that after oral administration. In 50 individual vein grafts performed on 35 patients, graft flow increased from 65 +/- 37 to 96 +/- 55 ml/min (p less than 0.001) after the dipyridamole injection and the arterial pressure decreased slightly. In 40 grafts whose graft flow was increased by more than 10 ml/min by dipyridamole, the patency rate (at 5 weeks) was 98 per cent, whereas that of the 10 other grafts, which responded poorly, was only 50 per cent (p less than 0.01). The serum concentration of dipyridamole, 3 minutes after intravenous injection, was 1.46 +/- 0.68 micrograms/ml, while the level of orally administered dipyridamole, in 3 groups of patients who were given 50 mg, 75 mg and 100 mg, three times a day, respectively, was steady, being 0.68 +/- 0.20 micrograms/ml, 1.43 +/- 0.41 micrograms/ml and 1.73 +/- 0.50 micrograms/ml, 2 hours following ingestion. We concluded that intravenous dipyridamole increases the graft flow and that a better patency is obtained in those grafts in which the graft flow is increased by more than 10 ml/min. It is also expected that routine doses of oral dipyridamole possibly increase the graft flow after coronary bypass surgery.

Administration, Oral↗
PubMed1988

Superoxide production in aprotic interior of chloroplast thylakoids.

The site of superoxide production in spinach thylakoids was found to be the aprotic interior of the thylakoid membranes near the P700 chlorophyll a protein at the reaction center of photosystem I complexes. This conclusion was drawn from the following findings. (i) Cytochrome c reduction by illuminated thylakoids, which was confirmed to be superoxide dependent by the failure of this reaction to occur in anaerobiosis, was completely inhibited by a dibutyl catechol, but partially inhibited by a hydrophilic disulfonated derivative. (ii) P700 chlorophyll a proteins were preferentially iodinated by lactoperoxidase by the use of hydrogen peroxide that was derived from the disproportionation of superoxides in illuminated thylakoids. (iii) Hydrogen peroxide production and oxygen uptake were induced by ammonium chloride, a proton conductor that can permeate through thylakoid membranes, but whole superoxide in the bulk solution was oxidized back to molecular oxygen by cytochrome c. The effective concentration of ammonium chloride decreased to one-sixtieth of the original, when an ammonium ion ionophore, nonactin, was added. Thus, the weak acid allowed superoxide to yield hydrogen peroxide disproportionately in the thylakoid membrane interior.

Ammonium Chloride↗
PubMed1988

[Considerations in determining indication for valvular surgery].

Between 1970 and 1987 valvular operations with cardiopulmonary bypass were performed on 636 patients, with an overall early mortality of 7.5%. To determine the critical indication for valvular surgery, preoperative clinical characteristics were examined by comparing 35 patients, who had died of surgery because of low cardiac output syndrome or multiple organ failure, with operative survivors. The multivariable logistic analyses demonstrated that NYHA functional class IV, multiple valvular disease, large CTR more than 70%, high left ventricular end-diastolic pressure more than 15 mmHg, depressed left ventricular ejection fraction less than 40%, and renal and hepatic dysfunction were powerful independent clinical characteristics concerning operative mortality. The mortality was highly significant when a patient was associated with more than three of the above factors in addition to being NYHA class IV. It should be emphasized, however, that there are three survivors among 11 patients with all six factors. On the other hand the prognosis of such patients without surgery was very poor, fifteen of 21 patients, medically treated during the same period, died within two years after reaching NYHA class IV condition. These results show that several predictors are useful for decision making regarding the indication for valvular surgery. Surgery, however, should not be denied because of the severity of the disease.

Heart Valve Diseases↗