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Biomedical subjects

K Austin

Publications and source records attributed to K Austin.

At least 19 recordsLinked to original sources

Biodiversity in urban habitat patches.

We examined the biodiversity of urban habitats in Birmingham (England) using a combination of field surveys of plants and carabid beetles, genetic studies of four species of butterflies, modelling the anthropochorous nature of the floral communities and spatially explicit modelling of selected mammal species. The aim of the project was to: (i) understand the ecological characteristics of the biota of cities model, (ii) examine the effects of habitat fragment size and connectivity upon the ecological diversity and individual species distributions, (iii) predict biodiversity in cities, and (iv) analyse the extent to which the flora and fauna utilise the 'urban greenways' both as wildlife corridors and as habitats in their own right. The results suggest that cities provide habitats for rich and diverse range of plants and animals, which occur sometimes in unlikely recombinant communities. The studies on carabids and butterflies illustrated the relative importance of habitat quality on individual sites as opposed to site location within the conurbation. This suggests that dispersal for most of our urban species is not a limiting factor in population persistence, although elements of the woodland carabid fauna did appear to have some geographical structuring. Theoretical models suggested that dormice and water voles may depend on linear habitats for dispersal. The models also indicated that other groups, such as small and medium sized mammals, may use corridors, although field-based research did not provide any evidence to suggest that plants or invertebrates use urban greenways for dispersal. This finding indicates the importance of identifying a target species or group of species for urban greenways intended as dispersal routeways rather than as habitat in their own right. Their importance for most groups is rather that greenways provide a chain of different habitats permeating the urban environment. We suggest that planners can have a positive impact on urban biodiversity by slowing the pace of redevelopment and by not hurrying to tidy up and redevelop brownfield sites.

Animals↗

Response of T lymphocytes from previously infected calves to recombinant Cryptosporidium parvum P23 vaccine antigen.

We had previously demonstrated that a Type-1-like immune response involving interferon-gamma expression in lamina propria lymphocytes accompanied by IgG2 subclass fecal antibodies to Cryptosporidium parvum p23 emerged in gut mucosa of calves recovering from cryptosporidiosis. Because a recombinant p23 had been shown to protect calves from cryptosporidiosis when administered as a vaccine antigen to late gestation cattle, this study was undertaken to determine if the same vaccine antigen could induce a Type-1-like, in vitro response by T cells from calves that had recovered from C. parvum infection. We isolated peripheral blood mononuclear cells from calves that had been previously infected with C. parvum oocysts and incubated them in the presence or absence of the recombinant C. parvum p23 vaccine antigen. We used flow cytometry to simultaneously detect cells in cell cycle and identify the T cell subset containing cycling cells. We also used flow cytometry to identify interferon-gamma positive cells and 2-dimensional gel electrophoresis to profile proteins made by PBMC stimulated with the recombinant p23 vaccine antigen. The results demonstrated that CD4+ T lymphocytes proliferated and that interferon-gamma was synthesized by a subset of stimulated cells. Two-dimensional gel electrophoresis revealed the presence of several cytoplasmic proteins in a size range of approximately 25-80 kDa that were detected in p23-stimulated, but not in unstimulated, cytoplasmic samples. Together, the results show that the recombinant p23 vaccine antigen can stimulate a Type-1-like immune response by T cells from calves that have recovered from C. parvum infection.

Animals↗

Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples.

The performance of the Virogen Rotatest latex agglutination test (LAT) was evaluated for detection of bovine rotavirus antigen. Sixty-three fecal samples from diarrheic calves were collected from November 1999 to May 2000 and screened by LAT, the Rotazyme II enzyme-linked immunosorbent assay (ELISA), and virus isolation (VI) followed by an anti-rotavirus fluorescent-antibody (FA) test to detect the presence of group A rotavirus antigen. Of the 63 samples screened by VI-FA, 33 (58%) tested positive for rotavirus antigen. When the results from the LAT were compared to those from VI-FA, the "gold standard" for detection of bovine rotavirus in fecal samples, the sensitivity and specificity were found to be 87.8 and 73.3%, respectively. Latex agglutination compared with ELISA (the reference method) showed 100% sensitivity and 96.3% specificity, and when ELISA was compared with VI, the sensitivity was 84.8% and the specificity was 73.3%. Latex agglutination is easy to perform in a short time and does not require expensive equipment or skilled personnel, and the reagents have long shelf lives. These factors make the LAT suitable and highly efficient for use in a clinical laboratory as a rapid screening test for bovine rotavirus.

Animals↗

A-dec as a partner to dentists.

A-dec has become the largest manufacturer of dental equipment in America by focusing on the customer, the employee, and the product rather than the bottom line. The driving principles of the company are (a) quality comes first, (b) customers are the focus of everything, (c) continuous improvement, (d) employee involvement is a way of life, (e) dealers and suppliers are our partners, and (f) integrity is never compromised.

Dental Equipment↗

Assessing the contribution of natural sources to regional atmospheric mercury budgets

Naturally mercury-enriched substrate is a long-lived source of mercury to the global atmospheric mercury cycle. Field flux chambers, laboratory gas exchange chambers and micrometeorological methods may be applied to estimate emissions from these sources. However, field chamber experimental design may affect the magnitude of the fluxes measured, and the laboratory chamber only provides a minimum estimate of flux. Many factors, such as mercury concentration and speciation in substrate, light, precipitation, and temperature, influence the emission of mercury from the substrate. Mercury concentration in the substrate is a dominant factor controlling emissions and may be used to predict emissions from regions of mercury enrichment. Mercury fluxes measured from three areas of natural enrichment and three areas with low levels of mercury enrichment are 1-5 orders of magnitude greater than the value applied to global belts of natural enrichment. Preliminary scaling of emissions from one of these areas and for western North America indicates that mercury enriched areas may be significant sources of mercury to the atmosphere, and that their contribution to regional and global atmospheric budgets needs to be reassessed.

Journal Article↗

Multiple single units and population responses during inhibitory gating of hippocampal auditory response in freely-moving rats.

Paired clicks were presented to awake, freely-moving rats to examine neuronal activity associated with inhibitory gating of responses to repeated auditory stimuli. The rats had bundles of eight microwires implanted into each of four different brain areas: CA3 region of the hippocampus, medial septal nucleus, brainstem reticular nucleus, and the auditory cortex. Single-unit recordings from each wire were made while the local auditory-evoked potential was also recorded. The response to a conditioning stimulus was compared to the response to a test stimulus delivered 500 ms later: the ratio of the test response to the conditioning response provided a measure of inhibitory gating. Auditory-evoked potentials were recorded at all sites. Overall, brainstem reticular nucleus neurons showed the greatest gating of local auditory-evoked potentials, while the auditory cortex showed the least. However, except for the auditory cortex, both gating and non-gating of the evoked response were recorded at various times in all brain regions. Gating of the hippocampal response was significantly correlated with gating in the medial septal nucleus and brainstem reticular nucleus, but not the auditory cortex. Single-unit neuron firing in response to the clicks was most pronounced in the brainstem reticular nucleus and the medial septal nucleus, while relatively few neurons responded in the CA3 region of the hippocampus and the auditory cortex. Taken together, these data support the hypothesis that inhibitory gating of the auditory-evoked response originates in the non-lemniscal pathway and not in cortical areas of the rat brain.

Animals↗

The identification of mistakes in road accident records: Part 1, Locational variables.

The current method of checking police-reported road accident data involves a rigorous process of manual and computer validation, with the objective of removing all the errors that exist on the accident report forms. This paper shows how a geographic information system (GIS) can be used to identify mistakes that exist in several locational variables once this process has been undertaken. It compares items contained on the accident report form with accurate highway feature information obtained from other sources. There were less than 10% of mistakes for the variables of district, speed limit, road class, and road number; less than 20% for junction control, junction detail, and pedestrian crossing facilities; and over 20% for carriageway type. If highway data were routinely entered onto a GIS by all British highway authorities, the above variables might not need to be entered by the police on their accident report forms.

Accidents, Traffic↗

The identification of mistakes in road accident records: Part 2, Casualty variables.

It has been acknowledged that there are many mistakes in recording the casualty variables on the police accident report forms. This study shows how a computer-based linkage of police and hospital records can be used routinely to identify the mistakes for the variables of casualty age, gender, and severity. The level of omissions and misidentifications were 0.4% for gender, 12.2% for severity, and 15.7% for age. The number of records that had age omitted or coded incorrectly was not significantly greater for seriously injured casualties than for those who were slightly injured. This system could be effectively used to code casualty severity using medical information instead of police judgement.

Accidents, Traffic↗

[The psychological factors that affect the making of a decision for surgical sterilization and the likely later changes].

The practice of female surgical sterilization in Panama during the past decades, motivated the realization of this study, to determine the factors which caused women to use this permanent contraceptive method, and to see if regrets later occurred after that decision, in the sterilized women. A sample of 151 women were selected, between the ages of 28 and 45 years, concurrently pregnant and with 2 or more living children at the moment of the study. Psychological tests were applied during three different phases (pre-operatively and subsequently 12 and 24 months, post operatively). Results showed that female sterilization did not cause long term complications, and amongst the majority of women conforming the sample, a stable psychological profile was found up to 24 months after the interventions, with a significant improvement in the last evaluation of the social adaptation variable, in comparison to the pre-operative assessment.

Adult↗

Spectral analysis of the electroencephalogram in the developing rat.

Power spectral measures of the EEG obtained from the frontal cortex and hippocampal formation during different vigilance states in the developing rat have been computed and compared. The most significant ontogenetic changes were observed in the hippocampal power spectra obtained during the vigilance state of REM sleep. These spectral analyses have revealed in the hippocampus: (1) a significant increase in the frequency at which the peak power occurs in the theta-frequency (4-11 Hz) band from 14 to 45 days of age; (2) a decrease in the quality factor of the peak from 14 to 45 days of age; (3) a decrease in the relative power co-ordinate for the center of spectral mass associated with the 0-4-Hz frequency band coupled with an increase in the frequency coordinate of the 4-11-Hz frequency band from 14 to 45 days of age, and; (4) a significant decrease in the average percent relative power associated with the 0-4-Hz frequency band from 14 to 22 days of age. For the EEG obtained from the frontal cortex, the major findings of note were: (1) a dominant contribution of relative power in the 0-4-Hz frequency band which was observed at every age and during every vigilance state tested, and; (2) a significant increase in the average percent relative power associated with this band at 18, 22, and 45 days of age. The results of this study provide a quantitative description of the electroencephalographic (EEG) ontogeny of the hippocampal formation and the frontal cortex in the rat. These ontogenetic changes in EEG activity relate closely to development of the internal circuitry and synaptic maturation in the hippocampal formation and frontal cortex.

Aging↗

Power spectral analysis of hippocampal and cortical EEG activity following severe prenatal protein malnutrition in the rat.

We have studied the effects of prenatal protein malnutrition on development of the hippocampal and frontal cortical electroencephalographic (EEG) activity. Using power spectral analyses in rats of several age groups we found that protein malnutrition, instituted prenatally and continued postnatally, produces marked alterations in power spectral measures, i.e., alterations in peak theta frequency in the hippocampus during rapid eye movement (REM) sleep. Peak theta frequency was found to be significantly retarded in malnourished animals, especially during the preweaning period of development. Protein malnutrition, therefore, appears to affect mechanisms responsible for generating the tonic component of theta activity.

Age Factors↗

Computer aids for floodlighting design.

The lighting engineer is faced with many problems when specifying floodlighting schemes, not least the large amount of computation required to evaluate each potential solution in terms of illuminance grids and isolux contours across the area of interest. Traditionally these kinds of evaluations have been carried out on (programmable) calculators with a design time days for anything other than trivial schemes. Mainframe computers are used in batch mode to ease this problem, but there is a need for an interactive system directly available in the design office. Interactive desk-top microcomputers with graphics facilities make this kind of system both technically and economically viable for the small design office. This paper describes the recently implemented system at Abacus Municipal Ltd, a company involved in the design, supply, and installation of a wide range of floodlighting systems. The primary objective is to provide the lighting engineer with a simple interactive computer system for specifying the details of a floodlighting scheme, leaving the computer to perform the relatively simple but tedious calculations to determine the illumination over the area of interest. User interaction in terms familiar to the lighting engineer is considered to be important so that the engineer's skills, knowledge and experience are enhanced rather than replaced. The design time using the system is typically reduced to a matter of hours rather than days, and improved and more precise results can be presented to the potential client.

Journal Article↗

Spectral analysis of neocortical and hippocampal EEG in the protein malnourished rat.

In these studies, power spectral analysis techniques were utilized to quantify changes in the cortical and hippocampal EEG obtained from rats reared on either an 8% or 25% casein diet as they mature from 14 to 22 days of age. Analyses of the power spectral data obtained from the frontal cortex of rats reared on the 25% casein diet during this preweaning period (14-22 days) indicated increases in the power in the 0.5-3.5 c/sec frequency band primarily during slow-wave sleep. Prenatal protein malnutrition (8% casein diet) significantly reduced the low frequency power content in the cortical EEG obtained during slow-wave sleep at 18 and 22 days of age. Analyses of power spectral data obtained from the hippocampal EEG during REM sleep also revealed developmental and diet-related differences. The frequency at which the peak power occurs in the theta band (4-11 c/sec) was found to increase normally in a linear fashion from 14 to 22 days of age (from 4.4 to 5.5 c/sec) in rats reared on the 25% casein diet. In addition, power in the 4-7 c/sec band significantly increased during this preweaning period. Prenatal protein malnutrition significantly slowed the development of theta frequency and produced higher values of power in the component of theta rhythm. Finally, vigilance profiles showed that rats normally progress from 14 days of age when REM sleep dominates their vigilance profile, to 22 days of age when they spend significantly more time in slow-wave sleep. Prenatal protein malnutrition retards this normal developmental sequence by significantly reducing the REM sleep time at 14 and 18 days of age and increasing the time spend in aroused waking at both these ages. However, at the time of weaning, i.e., at 22 days of age, these differences in sleep-waking behavior were no longer observed to be significant.

Animals↗

Endocrine-metabolic response to acute starvation in human gestation.

During a 72 hour fast in pregnant women, significant decrements in the maternal plasma glucose concentrations, accompanied by a significant increase in the plasma placental lactogen (hPL) concentration, occur. At the same time, utilization of glucogenic amino acids, principally alanine, takes place. The mean postprandial glucose concentration in pregnancy is significantly lower than that of comparable nonpregnant women (70.5 +/- 1.7 versus 79.5 +/- 1.3 mg. per 100 ml., p less than 0.001). There appears to be a significant sparing effect on the maternal plasma glucose concentration during acute fasting which may be mediated through hPL. Concentrations of amniotic fluid and fetal plasma glucose from women undergoing fasting decrease in a manner parallel to that of the mother. Fasting provokes a mean rise in plasma hPL of 33.2 per cent over basal levels. This rise is still evident 72 hours after refeeding, after which it gradually returns to pretest concentrations. The infusion of alanine or arginine to pregnant women at the end of the fast produced increments in the peripheral maternal glucose concentration. The response was much greater with alanine than with arginine, demonstrating the increased gluconeogenic potential of this amino acid. The increment in human growth hormone (hGH) following alanine infusion was significantly greater than that observed after arginine administration. Hypoaminoacidemia was present in nonpregnant and pregnant women in response to fasting, but the decline was greater in pregnancy. Acute fasting in the first half of gestation appears to produce significant alterations in carbohydrate metabolism evidenced by profound hypoglycemia, hypoinsulinemia, and hypoaminoacidemia. This maternal deficit can be reflected in fetal substrate concentrations. The effect of these changes on fetal growth and development is speculative at this time.

Abortion, Therapeutic↗