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K B Cooke

Publications and source records attributed to K B Cooke.

14 recordsLinked to original sources
PubMed1980

Further characterization of a melanoma-specific protein from human urine.

Isolation of a melanoma-specific protein (MSP) from human urine has been achieved using antibody affinity chromatography. MSP migrates as a single homogeneous protein on SDS PAGE and comparison of these data and ultracentrifuge analyses indicates that MSP contains a single polypeptide chain. MSP, however, shows considerable charge heterogeneity on isoelectric focusing. The desialo form, alpha 2 MSP, is found predominantly in patients with advanced metastatic disease, whilst only the sialo form alpha 1 MSP, is obtained from the urine of patients with early-stage disease. MSP does not react with antisera raised to alpha 1 foetoprotein (AFP) or carcinoembryonic antigen (CEA) and hence is immunologically distinct from these other tumour-associated glycoproteins. Antisera raised to MSP do not react with normal skin melanocytes nor with any foetal tissue tested, and hence the origin of MSP remains unresolved.

Antibody Specificity↗
PubMed1978

Melanoma specific protein: occurrence in the urine of patients with halo naevus and vitiligo.

Melanoma specific protein is immunologically related to altered naevus cell cytoplasm. It is excreted by patients with malignant melanoma but in no other malignancy. The protein has been detected in patients with actively developing halo naevi but not when repigmentation is taking place. It also occurs in patients with very active vitiligo but in no other pigment condition we have studied. It is suggested that the protein is a marker of active destruction of naevus cells by immune mechanisms and that the release of toxic materials during this immune reaction may be responsible for the production of the halo phenomenon and for the areas of vitiligo that may be seen elsewhere on the skin.

Adolescent↗
PubMed1978

Myelomatosis and the hyperviscosity syndrome.

Eleven patients are described in whom myelomatosis was complicated by the laboratory and clinical features of the hyperviscosity syndrome (HVS). The myeloma type was IgA in nine and IgG3 in two. In those patients with IgA myeloma the HVS was related to the presence of high molecular weight complexes in the serum. Remission of clinical features was obtained in all patients by plasma exchange. Clinical improvement coincided with reduction of whole blood viscosity and in those patients with IgA myeloma, with a parallel reduction of the high molecular weight complexes. The relationship between the IgA complexes and blood viscosity has been examined by physicochemical analysis of purified IgA monomer and polymers and evidence is presented to show that the IgA polymer has a higher intrinsic viscosity and axial ratio than the larger IgM molecule. The significance of these observations is discussed.

Blood Viscosity↗
PubMed1978

Sialic acids of human large bowel mucosa: o-acylated variants in normal and malignant states.

Five sialic acid variants have been demonstrated in normal, 'transitional', and tumour extracts of human large bowel mucosa by thin-layer chromatography. Two-dimensional thin-layer chromatography, with intermediate alkaline hydrolysis, confirmed that the variants were N-acetyl neuraminic acids with additional O-acylation. Cases studied so far show changes in the proportions of these variants in malignancy.

Acylation↗
PubMed1975

IgM paraproteins.

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Amyloidosis↗
PubMed1974

Changes in composition of mucin in the mucosa adjacent to carcinoma of the colon as compared with the normal: a biochemical investigation.

Fifteen surgical specimens from patients with carcinoma of the colon and rectum were studied. Scrapings from normal mucosa distant from the tumour and from macroscopically normal mucosa adjacent to the tumour (;transitional') were used for chemical estimation of hexosamines, sialic acid, and proteins. The presence of hexosamines and sialic acid was confirmed in both normal and transitional mucosa. Transitional mucosa showed increased levels of total hexosamines and sialic acid as compared with the normal and this was accompanied by an increase in neuraminidase-sensitive sialic acids. The present data have been compared with previous histochemical and autoradiographic studies and it is suggested that the changes described in the transitional mucosa are transformations representing an early stage of carcinogenesis.

Chromatography, Paper↗
PubMed1968

Elastofibroma.

Three cases of elastofibroma are described and the literature is reviewed. It is suggested that they are non-neoplastic lesions resulting from trauma. Their staining reactions and the response of the elastic type fibres to enzyme digestion suggest that these fibres are either true elastin or very closely related to it.

Aged↗