Biomedical subjects

K B DeOme

Publications and source records attributed to K B DeOme.

At least 19 recordsLinked to original sources

PubMed1984

Development and characterization of the BALB/cNIV mouse strain.

The strain BALB/cNIV/Crgl was developed by infecting BALB/c/Crgl mice with mouse mammary tumor virus from C3Hf mice. A BALB/c normal mammary duct was transplanted into the gland-free fat pad of a hormone-stimulated female C3Hf X BALB/c F1 mouse. A hyperplastic alveolar nodule was found in the BALB/c ductal outgrowth and was transplanted into another hybrid gland-free fat pad. The resultant hyperplastic alveolar outgrowth was finally transplanted to female BALB/c mice. The hyperplastic alveolar outgrowth contained an exogenous, infectious mouse mammary tumor virus named the nodule-inducing virus, which was thought to be derived from the endogenous low oncogenic mouse mammary tumor virus found in C3Hf mice. The hyperplastic alveolar outgrowth-bearing BALB/c mice were inbred for four generations, and one family was selected as the strain BALB/cNIV/Crgl. It was found that (a) the mouse mammary tumor virus found in the BALB/cNIV strain was milk transmitted, but not transmitted by infected males; (b) the BALB/cNIV breeding females had a low tumor incidence (40%) and a longer latent period (14 months) than did female BALB/cfC3H mice (92% at 8 months); (c) the BALB/cNIV nodule outgrowths had low tumor-producing capabilities (50%) and longer latent periods (13.4 months) than did nodule outgrowths derived from female BALB/cfC3H mice (100% at 7.7 months).

Animals↗

PubMed1982

Transplantation of mouse mammary epithelial cells grown in primary collagen gel cultures.

A technique for the transplantation of mouse mammary epithelial cells, grown in collagen gels, has been developed that demonstrates that the phenotype of the cells prior to culture was not altered by the culture conditions. Mammary epithelial cells from virgin and midpregnant C57BL/Crgl mice; virgin, midpregnant, and multiparous nonpregnant BALB/cfC3H/Crgl mice; a BALB/c hyperplastic alveolar nodule line, and mammary tumors from BALB/cfC3H/Crgl mice were embedded inside collagen gels and grown for 10 to 14 days in the presence of 25% swine serum plus cholera toxin (0.01 microgram/ml). The epithelial cells increased in number during the culture period. At the end of the culture period, the cells were removed from the collagen gels and transplanted to the gland-free mammary fat pads of 3-week-old syngeneic female mice. Culture in collagen gels increased the number of cells necessary to obtain a high percentage of mammary outgrowths as compared to cells not grown inside collagen gels. In general, mammary cells grown inside collagen gels gave rise to outgrowths, similar in phenotype to those from noncultured cells, and were representative of the tissue of origin. Mammary epithelial cells from C57BL/Crgl virgin donors grown in collagen gels for 10 to 14 days retained their ability to respond to the endogenous hormones of pregnancy and lactation of the host and formed lobuloalveolar structures full of secretion similar to the host's own mammary gland. The data indicate that the growth of mammary epithelial cells in collagen gels and subsequent transplantation into the gland-free fat pads of syngeneic hosts provides a simple system, wherein cells can be grown in vitro and their phenotypes determined in vivo.

Adenocarcinoma↗

PubMed1981

Recovery of transformed nodule and ductal mammary cells from carcinogen-treated C57BL mice.

Transformed nodule and ductal mammary cells were recovered by cell dissociation and transplantation of mammary cells from C57BL/Crgl mice treated with 7,12-dimethylbenz(a)anthracene. Four-week-old mice were divided into the following groups: Group A, not treated; Group B, 2 pituitary isografts under the kidney capsule; Group C, 1.0 mg 7,12-dimethylbenz(a)anthracene intragastrically at 5 and 6 weeks of age; and Group D, 2 pituitary isografts and 1.0 mg 7,12-dimethylbenz(a)anthracene intragastrically at 5 and 6 weeks of age. At 10, 14, 18, and 22 weeks of age, the mammary glands were enzymatically dissociated, and 10(5) cells were injected into the gland-free mammary fat pads of 3-week-old syngeneic mice. After 10 weeks, the outgrowths were examined and classified as ductal, ductal dysplasia, or hyperplastic alveolar nodule. Ductal dysplasias and hyperplastic alveolar nodule outgrowths were recovered from carcinogen-treated mice. Pituitary isografting enhanced the recovery of ductal dysplasia. Five serially transplanted dysplastic outgrowth lines were established and are their fifty and sixth transplant generations. The data demonstrate that transformed mammary gland cells can be removed from carcinogen-treated mice by means of cell dissociation and transplantation.

9,10-Dimethyl-1,2-benzanthracene↗

PubMed1978

Detection of inapparent nodule-transformed cells in the mammary gland tissues of virgin female BALB/cfC3H mice.

Inapparent nodule-transformed cells were recovered from morphologically normal mammary tissue from virgin female BALB/cfC3H/Crgl (mouse mammary tumor-positive) mice before the appearance of hyperplastic alveolar nodules (HAN) or tumors, by means of the cell dissociation technique. Mammary tissues were dissociated by means of collagenase (0.1%), hyaluronidase (0.1%), and pronase (1.25%). Aliquots of 10(5) viable cells in 0.01 ml medium were injected into the gland-free mammary fat pads of 3-week-old female syngeneic mice. After 10 weeks the outgrowths were examined and classified as ductal, HAN, tumor, or combinations of these types. The presence of HAN outgrowths indicated the presence of nodule-transformed cells in the donor mammary tissues. Nodule-transformed cells were recovered from 2-month-old donors, and the number of HAN outgrowths increased with donor age. Overt HAN and tumors did not appear in virgin female BALB/cfC3H/Crgl mice younger than 8 to 9 months of age. The data suggest that inapparent nodule-transformed cells occurred long before the appearance of HAN of tumors and that the numbers of transformed cells increased with donor age.

Age Factors↗

PubMed1978

Effect of parity on recovery of inapparent nodule-transformed mammary gland cells in vivo.

Inapparent nodule-transformed cells were recovered from five late-pregnant, first-pregnancy BALB/cfC3H females 4 months of age and from five late-pregnant multiparous females 6 to 7 months of age. Mammary tissues were removed from each donor and dissociated by means of the enzyme collagenase (0.1%), hyaluronidase (0.1%), and pronase (1.25%). Aliquots of 100,000 viable cells in 0.01 ml of media were injected into the gland-free mammary fat pads of 3-week-old syngeneic host mice. Ten weeks after the injection the outgrowths were classified as ductal, nodule, tumor, or combinations of these types of outgrowths. The recovery of nodule outgrowths indicated the presence of nodule-transformed cells in the cell suspension that was injected. All donors yielded nodule outgrowths, and the percentage of outgrowths was significantly greater than was the percentage recovered from virgin BALB/cfC3H females of the same age groups. The latent period for the emergence of nodules and tumors was reduced from 8 to 9 months in virgin females to 4 months in parous females. The incidence of both nodules and tumors was greatly increased. The data suggest that parity significantly increases the numbers of nodule-transformed cells in donor tissue, decreases the time required for the emergence of nodules and tumors, and increases the number of overt nodules and tumors.

Age Factors↗

PubMed1973