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Biomedical subjects

K B Jones

Publications and source records attributed to K B Jones.

14 recordsLinked to original sources
PubMed1991

AIDS law in effect in Pennsylvania.

The "Confidentiality of HIV-Related Information Act" signed by Governor Casey on November 29, 1990, became effective February 27, 1991. Hospitals have an additional 60 days--until April 28--to establish written confidentiality procedures. The Act's provisions are generally consistent with traditional medical consent and confidentiality concepts tailored to the special circumstances surrounding the human immunodeficiency virus (HIV) and the acquired immunodeficiency syndrome (AIDS).

Acquired Immunodeficiency Syndrome↗
PubMed1988

Amphibian oocyte maturation induced by extracts of Physarum polycephalum in mitosis.

The orderly progression of eukaryotic cells from interphase to mitosis requires the close coordination of various nuclear and cytoplasmic events. Studies from our laboratory and others on animal cells indicate that two activities, one present mainly in mitotic cells and the other exclusively in G1-phase cells, play a pivotal role in the regulation of initiation and completion of mitosis, respectively. The purpose of this study was to investigate whether these activities are expressed in the slime mold Physarum polycephalum in which all the nuclei traverse the cell cycle in natural synchrony. Extracts were prepared from plasmodia in various phases of the cell cycle and tested for their ability to induce germinal vesicle breakdown and chromosome condensation after microinjection into Xenopus laevis oocytes. We found that extract of cells at 10-20 min before metaphase consistently induced germinal vesicle breakdown in oocytes. Preliminary characterization, including purification on a DNA-cellulose affinity column, indicated that the mitotic factors from Physarum were functionally very similar to HeLa mitotic factors. We also identified a number of mitosis-specific antigens in extracts from Physarum plasmodia, similar to those of HeLa cells, using the mitosis-specific monoclonal antibodies MPM-2 and MPM-7. Interestingly, we also observed an activity in Physarum at 45 min after metaphase (i.e., in early S phase since it has no G1) that is usually present in HeLa cells only during the G1 phase of the cell cycle. These are the first studies to show that maturation-promoting factor activity is present in Physarum during mitosis and is replaced by the G1 factor (or anti-maturation-promoting factor) activity in a postmitotic stage. A comparative study of these factors in this slime mold and in mammalian cells would be extremely valuable in further understanding their function in the regulation of eukaryotic cell cycle and their evolutionary relationship to one another.

Animals↗
PubMed1985

Traumatic rupture of the hepatic duct demonstrated by endoscopic retrograde cholangiography.

Persistent jaundice and abdominal distention were noted postoperatively in a 37-year-old white male who had undergone splenectomy and suture of a liver laceration following a motorcycle accident. Abdominal paracentesis yielded bile-stained fluid. Endoscopic retrograde cholangiography (ERC) demonstrated rupture of the hepatic duct at its bifurcation which had been missed on initial laparotomy. ERC was valuable in planning and subsequently performing a hepatojejunostomy. We found no previous report of using ERC to diagnose and locate a missed bile duct injury.

Adult↗
PubMed1978

Development of normal and dystrophic chick muscle in tissue culture. Production and release of creatine kinase and acetylcholinesterase.

Creatine kinase (CK) and acetylcholinesterase (AChE) were followed during the course of development of embryonic muscle in tissue culture for periods of up to 8 weeks. Control muscle cultures released CK and AChE into the medium for the first 2-3 weeks, after which time measurable enzyme release ended. In dystrophic muscle cultures release of CK and AChE continued over most of the 8-week culture period. Cumulative measurable activity of CK was significantly greater than that of controls by the 3rd week and thereafter and cumulative AChE release was greater than that of controls by the 5th week and averaged about twice that of controls by the end of the culture period. Total cell CK activity of the dystrophic muscle cultures was greater than control values but results of cellulose acetate electrophoretic analysis of CK isozyme composition indicated that control muscle cultures attained higher percentage levels of MM isozyme type and lower levels of MB isozyme. Breast muscle extracts from adult dystrophic chickens had an approximately 10--13% content of MB isozyme while in similar extracts of control chicken muscle only the MM isozyme was detectable and total activity was greater than in dystrophics. Bound AChE activity of dystrophic muscle was significantly greater than control levels subsequent to the 2nd week in culture. Soluble AChE activity of dystrophic muscle was somewhat greater than comparable control activity in 2 of 3 tissue culture series at approximately the same period. In 7--10 week-old dystrophic chickens, both soluble and bound AChE activities of dystrophic breast muscle extracts were markedly increased over control values.

Acetylcholinesterase↗