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Biomedical subjects

K B Orr

Publications and source records attributed to K B Orr.

At least 19 recordsLinked to original sources
PubMed1992

Do not resuscitate.

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Cardiopulmonary Resuscitation↗
PubMed1990

An ELISA procedure to detect anti-double-stranded and anti-single-stranded DNA binding antibodies in connective tissue disorders.

A simple and sensitive enzyme-linked immunosorbent assay (ELISA) is described for the measurement of antibodies to native DNA (double-stranded) and to heat-denatured DNA (single-stranded) in sera of patients with connective tissue disorders. DNA bound to polyvinylchloride plates is incubated with serum samples, and antibodies to both single- and double-stranded DNA are detected by means of goat antihuman IgG alkaline phosphatase conjugate. The binding in individual sera is expressed as binding units per milliliter and refers to the absorbance in relation to the absorbance value obtained with a set of standards. The various parameters of the ELISA assay are described, and the results are compared with results of the Crithidia and Farr DNA antibody assays. Two case reports are discussed.

Adult↗
PubMed1986

Low-dose (diagnostic-like) x-ray as a cocarcinogen in mouse colon carcinoma.

To test the hypothesis that low-dose (diagnostic-like) radiation may play the part of a cocarcinogen in inflammatory bowel disease, we used four groups of BALB/C mice, (a control, dimethylhydrazine, dimethylhydrazine plus low-dose radiation, and low-dose radiation). We found no protective or carcinogen effects of the radiation in combination with dimethylhydrazine compared to dimethylhydrazine alone. This type of negative experimental finding is important in that individuals with inflammatory bowel disease have many diagnostic x-rays throughout life.

Animals↗
PubMed1986

Modified counterimmunoelectrophoresis procedure for easy identification of antibodies to extractable nuclear antigens.

Much effort is being expended to detect and to characterize the multiplicity of antibodies directed against extractable nuclear antigens in connective tissue diseases. Hemagglutination and counterimmunoelectrophoresis are highly sensitive but lack specificity and must be followed by double diffusion. Double diffusion, however, suffers from lack of sensitivity and often from lack of clarity for interpretation of the resulting precipitin lines. We describe here a modified counterimmunoelectrophoresis procedure utilizing offsetting wells, followed by diffusion, which provides the clarity of precipitin lines for easy interpretation as well as overnight interpretation and the use of small amounts of reagents.

Antibodies, Antinuclear↗