[Experimental studies of the anticariogenic action of glass ionomer cement].
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Biomedical subjects
Publications and source records attributed to K Bössmann.
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Methacrylate embedding was suggested for improving the histologic examination of teeth with surrounding alveolodental ligament, including large sections of the jaw bone. The method introduced here makes it possible to prepare sections of decalcified as well as undecalcified material which, for the most part, are free of artefacts.
Conditioning the enamel is extremely important for the prophylactic treatment of caries with sealers. The possibility of dentition depends on the depth and pattern of etching. Incisors and molars were etched with seven etching agents. The depth of etching was examined via microradiography; the etching pattern, with the scanning electron microscope. The best etching agent proved to be a 50% solution of H3PO4 + 2% solution of NaF; The depth of etching was greater in the molars than in the incisors. The etching pattern produced with this agent was also the most even. The etching pattern could not be predicted on the basis of the etching agent.
The mechanisms contributing to plaque formation have still not been adequately explained. An attempt therefore was made to form plaque in vitro in order to acquire a model for the processes and what influences them. Plaque forms in various stages, analogous to the natural condition when saliva and microorganisms are present on the surface of the teeth. Microorganisms from plaque formed in situ demonstrate a decreased ability for adhesion and cohesion when compared with free salivary microorganisms. Sucrose encourages more rapid and thicker plaque formation.
The adhesiveness of plastic sealers have only been studied in face-ground enamel specimens. A procedure for examining adhesiveness in naturally formed enamel surfaces is described. After treatment with varying concentrations of phosphoric acid, three naturally formed occlusal surfaces were sealed with Enamite, Nuva-Seal, and Alpha-Seal. The best adhesiveness was obtained after conditioning with 50% H3PO4, without additives. The best values were achieved with Nuva-Seal; the worst, with Enamite. Additives to the cauterizing agent distinctly reduced adhesiveness.
Plastic sealers on the tooth are effective for no more than two years because of mechanical and thermal stress. Three plastics with different chemical compositions (Nuva-Seal, Alpha-Seal, and Enamite) therefore were examined in terms of abrasion. Necessary resealing results in thicker layers of sealer in the nonabrased areas, as determined with the three above-mentioned preparations. Bis-EMA type sealers satisfactorily resisted abrasion and also form the thinnest sealing layers.
A histological examination of solid tissue was undertaken to find out whether the appearance of border caries of fillings performed in situ and in vivo differs or looks the same. The results prove that secondary caries can be produced experimentally, and that the same mineral loss occurs as in secondary caries occurring under natural conditions. It occurs in 2 forms: the outer lesion and the lesion of the cavity wall. In addition results were obtained on the breadth of border clefts, forms of preparations and the quality of fillings and underfillings.
The genesis of dental caries and marginal periodontopathies has a conclusive connection with dento-gingival plaque. Plaque is a constantly regenerating accumulation of salivary constituents and especially of various oral micoorganisms. Microbial decomposition of sugars from the food produces acids from fermentation which lead to local demineralization of the teeth and carious destruction. Antigenic plaque substances cause immunological reactions. With persistent bad oral hygiene, a gingivitis may develop into a progressive periodontitis with deepened gingival pockets.
Under the electron microscope isolated mitochondria were found in the odontoblastic processes in the dentin up to the dentinoenamel junction. The larger number of mitochondria in the dentin third next to the pulp explains the finding by other authors of a localized activity of mitrochondrially bound enzymes in this part of the dentin.
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Comparative clinical studies on the caries incidence of 10-year-old school children in Kiel and Landskrona (Sweden) showed after statistical evaluation that the Landskrona children had lesser DF surfaces than the Kiel children. A comparison of incidence broken up into separate D and F surfaces showed that the Kiel children had more D surfaces, while the children of Landskrona had more F surfaces. The connection between caries incidence and prophylactic measures is discussed.