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K BENSCH

Publications and source records attributed to K BENSCH.

12 recordsLinked to original sources
PubMed1964

GRANULAR PNEUMOCYTES: ELECTRON MICROSCOPIC EVIDENCE OF THEIR EXOCRINIC FUNCTION.

The contents of the lamelar bodies of granular pneumocytes are normally released into the alveolar lumen. Exposure of guinea pigs to an atmosphere containing carbon dioxide causes the formation of abnormal lamellar bodies and a significant increase in the pulmonary surface tension. The eventual return to a normal pulmonary surface tension coincides with the formation of normal lamellar bodies.

Alveolar Epithelial Cells↗
PubMed1964

THE FATE OF DNA-CONTAINING PARTICLES PHAGOCYTIZED BY MAMMALIAN CELLS.

Particulate DNA protein coacervates were digested immediately after being phagocytized by L strain fibroblasts in suspension culture. Enlargement of the phagocytotic vacuoles occurred simultaneously with a loss of the electron opacity of the phagocytized particles. Cytochemical reactions positive for non-specific esterase, acid phosphatase, and nucleoside phosphatase in the phagocytotic vacuoles provided additional evidence for the probability of complete hydrolysis of the phagocytized nucleoprotein.

Acid Phosphatase↗
PubMed1963

Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.

THE ALDEHYDES INTRODUCED IN THIS PAPER AND THE MORE APPROPRIATE CONCENTRATIONS FOR THEIR GENERAL USE AS FIXATIVES ARE: 4 to 6.5 per cent glutaraldehyde, 4 per cent glyoxal, 12.5 per cent hydroxyadipaldehyde, 10 per cent crotonaldehyde, 5 per cent pyruvic aldehyde, 10 per cent acetaldehyde, and 5 per cent methacrolein. These were prepared as cacodylate- or phosphate-buffered solutions (0.1 to 0.2 M, pH 6.5 to 7.6) that, with the exception of glutaraldehyde, contained sucrose (0.22 to 0.55 M). After fixation of from 0.5 hour to 24 hours, the blocks were stored in cold (4 degrees C) buffer (0.1 M) plus sucrose (0.22 M). This material was used for enzyme histochemistry, for electron microscopy (both with and without a second fixation with 1 or 2 per cent osmium tetroxide) after Epon embedding, and for the combination of the two techniques. After fixation in aldehyde, membranous differentiations of the cell were not apparent and the nuclear structure differed from that commonly observed with osmium tetroxide. A postfixation in osmium tetroxide, even after long periods of storage, developed an image that-notable in the case of glutaraldehyde-was largely indistinguishable from that of tissues fixed under optimal conditions with osmium tetroxide alone. Aliesterase, acetylcholinesterase, alkaline phosphatase, acid phosphatase, 5-nucleotidase, adenosine triphosphatase, and DPNH and TPNH diaphorase activities were demonstrable histochemically after most of the fixatives. Cytochrome oxidase, succinic dehydrogenase, and glucose-6-phosphatase were retained after hydroxyaldipaldehyde and, to a lesser extent, after glyoxal fixation. The final product of the activity of several of the above-mentioned enzymes was localized in relation to the fine structure. For this purpose the double fixation procedure was used, selecting in each case the appropriate aldehyde.

Aldehydes↗