Biomedical subjects
Publications and source records attributed to K Baczko.
Suspension cultures of a human prostate cell line (MA160) supported abundant growth of the Edmonston strain of measles virus. The virus yields obtained with these suspension cultures (150 to 800 PFU/cell) were at least 20- to 100-fold higher than those frequently reported in the literature. Monolayer cultures of MA160 cells did not support a virus replication nearly as efficiently (progeny yield, 25 PFU/cell).
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In KB cells productively infected with adenovirus type 2, alkali-stable greater than 100S and 40-100S viral DNAs are synthesized starting 2-4 hr postinfection, i.e., before unit length (34 S) viral DNA is made. The amount of greater than 100S and 40-100S viral DNA increases when 34S viral DNA synthesis begins, and at 16-18 hr postinfection, the 40-100S viral DNA represents 5-20% of the total intracellular viral DNA. The 40-100S viral DNA is synthesized throughout infection. Part of the 40-100S DNA synthesized 5-8 hr postinfection has a density in alkaline CsCl gradients intermediate between those of viral and cellular DNAs. This finding indicates that newly synthesized viral DNA is covalently linked to cellular DNA. Viral sequences can be excised from the cellular DNA of infected cells with the EcoRI restriction endonuclease. Fragments of viral DNA are detected in polyacrylamide-agarose gels by DNA-DNA hybridization, and these fragments correspond in size to most of the known EcoRI fragments of adenovirus 2 DNA. Viral DNA sequences in size-classes between the EcoRI-A and -C fragments are also found and probably represent viral DNA linked to cellular sequences.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
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A recent examination of measles virus mRNA molecules has shown that the nucleocapsid and haemagglutin messengers are of the size expected from a consideration of their protein products. However, the mRNA for membrane protein is approximately 50% larger than the size required. The molecular weight of matrix protein has been determined by SDS-polyacrylamide gel electrophoresis, and this procedure can lead to an underestimation of the true size of hydrophobic molecules which show increased SDS binding. It is therefore appropriate to examine the molecular weight determination of this protein to exclude such an artefactual discrepancy in mRNA and protein sizes. We report here that measles virus membrane protein does not shown such anomalous behaviour and confirm that the size discrepancy is a true phenomenon.