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K Bandyopadhyay

Publications and source records attributed to K Bandyopadhyay.

18 recordsLinked to original sources
PubMed2005

Maximum entropy and the problem of moments: a stable algorithm.

We present a technique for entropy optimization to calculate a distribution from its moments. The technique is based upon maximizing a discretized form of the Shannon entropy functional by mapping the problem onto a dual space where an optimal solution can be constructed iteratively. We demonstrate the performance and stability of our algorithm with several tests on numerically difficult functions. We then consider an electronic structure application, the electronic density of states of amorphous silica, and study the convergence of the Fermi level with increasing number of moments.

Journal Article↗
PubMed2001

Role of 67 kDa cell surface laminin binding protein of Leishmania donovani in pathogenesis.

The role that interaction with laminin may play in Leishmania donovani infection was investigated. Binding of (125)I-radiolabeled laminin, in a liquid-phase assay, by the parasite was rapid, saturable, specific, reversible, and of high affinity. Using a Western blotting procedure, a 67 kDa laminin-binding protein (LBP) was identified from the membrane of both the promastigote and amastigote forms of L. donovani. Subsequently, the protein was purified by affinity chromatography. Immunofluorescence with a polyclonal antibody against LBP as well as flow cytometric analysis demonstrated its presence at the parasite surface. After stimulation with phorbol-12-myristate-13-acetate (PMA), U937 cells exhibited the ability to adhere to laminin and LBP specifically inhibited this adhesion. The reduced parasite adhesion after tunicamycin treatment suggested the importance of sugar residues in cell adhesion. Although co-administration of either laminin or LBP or anti LBP antibody reduced parasite virulence, resulting in a lower level of infection in the BALB/c mouse model, an in vitro macrophage culture-enhanced level of infection was observed in the case of laminin-coated parasites. The results collectively suggest a role for LBP in the interaction of the parasite with extracellular matrix elements, which may constitute a basis for the homing of the parasite to its physiological address.

Animals↗
PubMed2000

Ion recognition at the interface of self-assembled monolayers (SAMs) of bis-thioctic ester derivatives of oligo(ethyleneglycols).

Self-assembled monolayers (SAMs) of bis-thioctic ester derivatives of oligoethylene glycols were prepared. When the number of (-CH2-CH2-O-)n units in these podands was either five or six, the corresponding SAMs showed ion binding properties and selectivities similar to those exhibited by 15-crown-5 or 18-crown-6 in aqueous solution, respectively. Impedance data for the SAMs as a function of metal ion concentrations were fitted by using a Langmuir isotherm to determine the association constants (Ka) with the different ions. The SAM derived from the n = 5 compound is selective for Na+ while that with n = 6 is selective for K+. Results presented here confirm the formation of ion recognition domains during self-assembly of acyclic polyethylene glycol derivatives on gold surfaces; this suggests that surface-confined pseudocrown ether structures are formed.

Journal Article↗
PubMed2000

Dithia-crown-annelated tetrathiafulvalene disulfides: synthesis, electrochemistry, self-assembled films, and metal ion recognition

The synthesis and electrochemistry of a series of tetrathiafulvalene (TTF) and dithia-crown-TTF derivatives attached with one or two disulfide group(s) 7a-f are reported. The self-assembled monolayers (SAMs) of these TTF disulfides on gold were prepared and characterized by reflection-absorption infrared spectroscopy. The SAMs are extremely stable under a wide variety of conditions and over extended periods of time and show remarkable electrochemical stability upon repeated potential scans. SAMs of the crown-TTF disulfides 7c,d,f can recognize alkali metal ions, and the process can be monitored following the electrochemical potential shift of the surface-confined TTF group.

Journal Article↗
PubMed2000

Disaster recovery planning by HMOs: theoretical insights.

HMOs are becoming increasingly reliant on health management information systems (HMISs) for their effective functioning, competitive viability, and survival. Because of this critical dependence, HMOs must use disaster recovery planning (DRP) to safeguard their HMIS assets from natural as well as man-made disasters. This article purposes a theoretical framework, based on the theory of organizational innovation, to explain the factors involved in the adoption of DRP by HMOs.

Administrative Personnel↗
PubMed1999

Self-assembled Monolayers of Two Aromatic Disulfides and a Diselenide on Polycrystalline Silver Films: An Investigation by SERS and XPS.

Self-assembled monolayers of diphenyldisufide (DDS), naphthalenedisulfide (NDS), and diphenyldiselenide (DDSe) on polycrystalline silver films have been investigated by surface enhanced Raman spectroscopy (SERS) and X-ray photoelectron spectroscopy (XPS). DDS adsorbs on Ag through a homolytic cleavage of the S-S bond and resultant thiolate at the surface decomposes upon prolonged exposure to air. The geometry of the molecule is such that the benzene ring is almost horizontal to the surface. The Raman spectrum has been assigned in the light of ab-initio molecular orbital calculations. In DDSe, the Se-Se bond is retained upon adsorption and the molecule sticks up. In contrast, NDS is highly reactive on the microscopically rough surface so that a stable monolayer could not be prepared. A temperature dependent Raman study of the DDS monolayer shows the absence of any reorientation at the surface as one would expect from the adsorption geometry. XPS study complements the SERS data and shows the presence of Ag2S on an NDS exposed surface. Copyright 1999 Academic Press.

Journal Article↗
PubMed1999

Isolation of a laminin-binding protein from the protozoan parasite Leishmania donovani that may mediate cell adhesion.

Extracellular matrix (ECM)-binding proteins on the surface of Leishmania are thought to play a crucial role in the onset of leishmaniasis, as these parasites invade mononuclear phagocytes in various organs after migrating through the ECM. In a previous report, we presented several lines of evidence suggesting that Leishmania has a specific receptor for laminin, a major ECM protein, with a Kd in the nanomolar range. Here we describe the identification, purification and biochemical characterization of the Leishmania laminin receptor. When the outer membrane proteins of L. donovani were blotted on to nitrocellulose paper and probed with laminin, a prominent laminin-binding protein of 67 kDa was identified. The purified protein was isolated by a three-step process involving DEAE-cellulose, Con A (concanavalin A)-Sepharose and laminin-Sepharose affinity chromatography and was used to raise a monospecific antibody. The same protein was obtained when parasite membrane extracts were adsorbed to antibody affinity matrix and eluted with glycine. The affinity-purified protein bound to laminin in a detergent-solubilized form as well as after integration into artificial bilayers, and was subsequently characterized as an integral membrane protein. Metaperiodate oxidation and metabolic inhibition of glycosylation studies indicate the binding protein to be glycoprotein in nature and that N-linked oligosaccharides play a part in the interaction of laminin with the binding protein. Surface-labelled parasites attached to microtitre wells coated with laminin and the 67 kDa protein blocked the adhesion to laminin substrate. We propose that the 67 kDa protein is an adhesin involved in the attachment of Leishmania to host tissues.

Animals↗
PubMed1998

Effect of Co-Adsorbed Surfactant on the Structure of Self-Assembled Monolayer of Thiol on Polycrystalline Gold.

The structure and formation kinetics of a self-assembled monolayer (SAM) of long-chain alkane thiol in the presence and absence of an anionic surfactant like sodium dodecyl sulfate (SDS) has been studied using impedance, cyclic voltammetic, and quartz crystal microbalance (QCM) measurements. The comparison of these results, especially the ionic permeability of the monolayer, suggests a composite monolayer where both the surfactant and thiol molecules are cooperatively adsorbed to form distinct surfactant patches. This is further substantiated by the voltammetric experiments with redox probe in solution. A gradual change in the double layer capacitance value for the thiol monolayer alone (1.4 µF/cm2) to the composite monolayer (6.7 µF/cm2) and to the surfactant alone (11 µF/cm2) further supports the nature of the mixed monolayer. The kinetics of monolayer formation also shows interesting changes as revealed by the QCM studies, where a phase transition from simple to composite monolayer has been observed around an approximate coverage of 0.44. The same growth scheme is also observed for 1-pentanethiol, naphthalene disulfide, and diphenyl disulfide, suggesting that the mechanism appears to be general. Copyright 1998 Academic Press.

Journal Article↗
PubMed1996

Evidence of a laminin binding protein on the surface of Leishmania donovani.

Both the promastigote and amastigote forms of the intracellular parasite, Leishmania donovani bind the basement membrane glycoprotein laminin with high affinity (Kd = 3.56 x 10(-9) M and 3.98 x 10(-9) M respectively) with approximately 9000 and approximately 800 sites per cell. Bound laminin was identified by direct autoradiography and the binding protein through analysis of the parasite extract by SDS-PAGE and immunoblotting. A major component of 67 kDa was detected. The same protein was obtained when parasite outer membrane proteins were adsorbed to laminin-sepharose affinity matrix and subsequently eluted with SDS. The binding affinity of the isolated receptor was similar to that of the whole cells. Such a receptor isolated in Leishmania for the first time, may function as one of the bridging molecules for extracellular matrix recognition.

Animals↗