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K Basak

Publications and source records attributed to K Basak.

5 recordsLinked to original sources
PubMed1976

Effect of some inhibitors on kanamycin formation by Streptomyces kanamyceticus.

The formation of kanamycin is markedly inhibited by mercuric chloride, sodium iodoacetate, 2,4-dinitrophenol, sodium arsenite and sodium azide particularly when these are added at the start of fermentation. Less inhibition of kanamycin synthesis is observed in case of sodium 5,5-diethylbarbiturate, malonic acid, sodium arsenate and sodium fluoride. Inhibition of kanamycin synthesis is associated with growth inhibition in case of 2,4-dinitrophenol, sodium arsenite and sodium azide. Bacitracin and D-cycloserine have a stimulatory effect on kanamycin synthesis with slight inhibition of cellular growth. This stimulation might be due to accumulation of cell wallintermediates--aminosugar and sugar--which are shunted to the pathway of kanamycin synthesis. Penicillin lowers kanamycin synthesis by 65 percent as compared with 19 percent reduction of cellular growth. Chloramphenicol has a stimulatory effect at lower concentration (20 mug/ml), when it is added at 24 h of fermentation. At higher concentration (50 mug/ml) chloramphenicol shows marked inhibition of both cellular growth and antibiotic biosynthesis.

Arsenic↗
PubMed1975

Mineral nutrition of Streptomyces kanamyceticus for kanamycin formation.

Kanamycin production by Streptomyces kanamyceticus ATCC 12853 requires magnesium sulfate and potassium phosphate at concentrations of 0.4 and 1.0 g per liter, respectively. The optimal concentrations of Fe and Zn for production of kanamycin are 0.25 and 0.575 mug/ml, respectively, whereas Mo at 0.04 mug/ml allows maximal cellular growth and antibiotic synthesis. Mn and Ca are without any effect. Cu, Co, Ni, and V have inhibitory effect on growth of the organism as well as kanamycin formation.

Calcium↗
PubMed1973

Utilization of carbon and nitrogen sources by Streptomyces kanamyceticus for kanamycin production.

To select a suitable synthetic medium for kanamycin production, we tested a number of carbon and nitrogen compounds for their effect on growth of Streptomyces kanamyceticus ATCC 12853 as well on kanamycin production. Galactose was found to be the most suitable carbon source, though dextrin, soluble starch, and potato starch gave moderate yields. Sodium nitrate and glycine were adequate nitrogen sources for kanamycin production. There was, however, no direct relation between the growth of the organism and antibiotic formation. The pH of the medium might be an important factor for kanamycin formation, as media giving high kanamycin yields showed an alkaline pH without exception.

Alcohols↗