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Biomedical subjects

K Becker

Publications and source records attributed to K Becker.

At least 55 records · Page 3Linked to original sources
PubMed1988

Influence of the specific antagonist of PAF-acether, BN 52021, and of Ginkgo extract on the rejection of murine tail skin allografts and the PAF-acether mortality in mice in particular consideration of the role of TXB2.

An oral application of 2 x 10 mg/kg daily of the PAF antagonist, BN 52021, significantly prolonged the distal allograft survival in the murine tail skin model of cell-mediated allograft rejection. BN 52021 diminished the TXB2 content in the skin at and near to the transplantation site by two thirds whereas PAF-acether significantly enhanced the TXB2 content in murine tail skin. Similar to the tail skin BN 52021 also reduced by more than 70% the TXB2 release from human granulocytes stimulated by calcium ionophore A 23187. The PAF-acether mortality (LD75) in NMRI mice was decreased to 20% by BN 52021 with a lower dose than by BN 52020. Comparing Ginkgo total extract in doses which significantly diminished the 75% mortality by PAF-acether in mice with BN 52021 and BN 52020 in doses that produced the same inhibitory effect, a potentiated effect of Ginkgo extract resulted with respect to the share of BN 52021 and BN 52020 in Ginkgo extract.

Animals↗
PubMed1988

Influence of inhibitors of the eicosanoid metabolism, of antagonists of the eicosanoids and of PAF on mortality assayed in three biochemically characterized shock models.

Experiments were carried out to lower the mortality (LD70-90) of rats in ovalbumin-induced anaphylactic (DA) shock and in endotoxin-induced (ET) shock, and of mice after injection of Platelet-activating Factor (PAF shock) comparing the effects of the cyclooxygenase (COX)-inhibitors aspirin (ASA), indomethacin, of the COX-/lipoxygenase (LOX)-inhibitors nordihydroguajaretic acid (NDGA), phenidone and X 86 (analogue of BW 755c), of the inhibitor of thromboxane (TX) synthesis HOE 944, of the TX-antagonist BM 13177, of the PAF-antagonist BN 52021 and of ketotifen. Ketotifen was strongly effective in DA shock, COX- and LOX-inhibitors only slightly. Combined COX- and LOX-inhibitors and BN 52021 showed good effects in the ET shock. Ketotifen was inefficacious. All the used substances influenced the PAF shock. The shock syndromes were biochemically characterized by determination of isocitratedehydrogenase (ICDH) activity, lactate, glucose, haematocrit, numbers of thrombocytes and leucocytes, TXB2 and 6-keto-Prostaglandin(PG)F1 alpha.

Anaphylaxis↗
PubMed1988

Influence of prostaglandins and PG-analogues on the mortality of PAF-acether in mice and the TXB2 release from human granulocytes.

The PAF-acether mortality in female mice varied with strains: AB mice were very resistant, NMRI mice showed dose-dependent effects. We used an i.v. injection of 75 micrograms/kg PAF-acether resulting in a 75% mortality in NMRI mice. Nileprost and PGE1 in one tenth of the doses of iloprost or PGI2 were able to reduce the PAF-acether mortality. Nalador, PGE2 and flunoprost were ineffective. 10 min after PAF application the TXB2 content in tail skin was increased significantly. The inhibitory effect on the TXB2 release induced by A 23187 from human granulocytes was stronger by nileprost than by iloprost. Nalador was ineffective, flunoprost enhanced the release. The lowered TXB2 formation is a possible explanation for the prevention of the PAF-acether induced death by PGs.

6-Ketoprostaglandin F1 alpha↗
PubMed1987

[Everyday psychiatry--a contribution to the hermeneutics of diagnostic and therapeutic clinical practice].

The relations between diagnostic and therapeutic practice and the events of everyday life are investigated in a two-fold regard. The first view is directed towards the signification of "psychiatry" and of the psychiatric view-points in the everyday life of society; the second view is the experience of everyday life inside psychiatric institutions. Under both aspects an interest in the development and the both aspects an interest in the development and the process of mental illness is pursued, which is also an interest in correct diagnostic insight and in promoting therapeutic consequence that positively influences the process.

Adaptation, Psychological↗
PubMed1987

[Cardia cancer: attempt at a therapeutically relevant classification].

On the basis of a prospective analysis of 107 adenocarcinomas of the gastro-esophageal junction we propose a differentiation into adenocarcinomas of Barrett's esophagus (endobrachyesophagus), "real" carcinoma of the cardia, and subcardial carcinoma of the fundus. This classification allows the determination of an adequate surgical resection. Therefore the operative risk can be kept low. Total esophagogastrectomy is reserved only for advanced "real" carcinomas of the cardia in this concept.

Adenocarcinoma↗
PubMed1986

Rescue of a tk-plasmid from transgenic mice reveals its episomal transmission.

This communication demonstrates the usefulness of the plasmid rescue procedure for recovery of plasmids from transgenic mice. We have microinjected the plasmid pSK1 harbouring the Herpes simplex virus thymidine kinase gene into fertilized mouse oocytes and succeeded in recovering plasmids from newborns by transformation of E. coli either with HindIII cut cellular DNA or with uncut DNA. The majority of the rescued plasmids were indistinguishable from pSK1 by restriction analysis. The rescued plasmids proved to be functionally active in a transient expression assay in mouse Ltk- cells. The pSK1 DNA sequences were inherited by up to 90% of the second generation progeny mice, which is not in agreement with a Mendelian transmission of heterozygous markers integrated into a single site of the chromosome. These data support the assumption that germ line transmission of non-integrated episomal plasmids can occur.

Animals↗
PubMed1986

Evaluation of the Coulter S-Plus V three-part differential in a community hospital, including criteria for its use.

The Coulter S-Plus V three-part differential was evaluated for its precision, correlation with 100-cell manual differentials, ability to process routine specimens without flagging, and ability to detect abnormalities as defined by manual differential. The instrument's precision is markedly greater than that of manual methods, especially in the mononuclear category. Correlation with manual differential lymphocyte, mononuclear, and granulocyte percentages was 0.91, 0.54, and 0.94, respectively. Eighty-four percent of routine samples could be processed by the instrument without further review. With the use of a combination of S-Plus V-generated flags and preset laboratory criteria, only 9% of abnormal manual differentials would have been undetected. Three-fourths of these undetected cases involved low-frequency abnormalities of questionable clinical significance. The three-part differential should be able to decrease the number of manual differentials that are performed, which would result in a cost savings to the laboratory.

Cell Count↗
PubMed1986

[Social environment of alcoholic patients after clinic discharge and its effect on treatment success].

The study concerns the social situation of alcoholics after a 3- to 6-weeks in-patient withdrawals. As central question appears that for the influence of the social situation on treatment outcome. We distinguish between the life-subjects work and occupation, partnership, spare time, living situation, financial situation and official and judicial problems. In order to register the treatment outcome we had catamnestic interviews 3 months after the patients left the hospital. The sample consist of 48 patients. The most important result is: the more disadvantageous the social situation, the patients relapse. Having an employment, a good partnership and enough income is must important for the treatment outcome.

Adult↗
PubMed1986

[New observations in a case of Cronkhite-Canada syndrome].

We report about a 76 years old patient with Cronkhite-Canada syndrome. The diagnosis has been found with the following clinical symptoms: diarrhea, anorexia, alopecia, and onychotrophia. Laboratory values: severe hypoproteinemia (total serum protein 4.3 g/dl, albumin 2.4 g/dl); endoscopical and radiological findings: a generalized polyposis which involved the whole intestine except the oesophagus. As far as we saw in our literature-overview of 55 patients with Cronkhite-Canada syndrome, this patient had for the first time a carcinoma of the urinary bladder and a Bricker operation 17 years before the onset of his disease. Further we remarked a lack in the resorption of the enterally administered thyroidal hormones. The progress was fatal despite a parenteral hyperalimentation and a treatment with antibiotics and glucocorticoids.

Aged↗
PubMed1985

Comparative column chromatographic estimations of phenylalanine in plasma, whole blood, native and paper-dried capillary blood of healthy children and adults, and patients with hyperphenylalaninaemia.

The concentration of phenylalanine in plasma, whole venous and capillary blood, and paper-dried blood of 75 probands (25 healthy adults, 27 healthy children, and 23 patients with hyperphenylalaninaemia) were measured by use of a sensitive short column chromatography method. The comparison of the values in each group of probands by several statistic methods showed an excellent correlation of the phenylalanine concentration in paper-dried whole blood to those measured in venous plasma. Evaluation of the analytical method revealed a high sensitivity and accuracy by use of a sample volume of 50 microliter. We would therefore suggest that the estimation of phenylalanine for the diagnosis and therapy control in hyperphenylalanine is as accurate in paper-dried blood as in venous plasma and would simplify sampling for the patients as well as enhance the diagnosis and therapy control in hyperphenylalaninaemia.

Adult↗
PubMed1985

IncP-1 R plasmids decrease the serum resistance and the virulence of Pseudomonas aeruginosa.

Pseudomonas aeruginosa strain PAO1 was compared to PAO1 strains containing an IncP-1 R plasmid (RP1, R68, or R68.45) in an experimental mouse burn infection model. All R plasmids tested caused a 10- to 400-fold increase in mean lethal dose (LD50). The decrease in virulence produced by plasmids R68 and R68.45 was significantly greater than the decrease caused by the closely related plasmid RP1. All plasmids also led to an increased sensitivity of strain PAO1 to human serum bactericidal activity. Virulence and serum resistance of strain PAO1 were restored by curing of the entire plasmid R68.45 but not by deletions in the plasmid's transfer gene regions.

Animals↗