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Biomedical subjects

K Belej

Publications and source records attributed to K Belej.

At least 19 recordsLinked to original sources

[Morphologic study of mastocytes from skin biopsies in patients with non-systemic and systemic mastocytosis].

The authors describe morphologic changes in mastocytes in the picture of transmission electron microscopy (TEM) and skin changes in patients with urticaria pigmentosa (7 causes) and systemic mastocytosis (2 causes). The presented study analyzes the changes in the cellular membrane and cytoplasmic processes, intracytoplasmic membrane structures, mitochondria, granules, and nuclei of mastocytes in patients with systemic mastocytosis. The authors evaluate these cells as being atypical, respectively immature. The study discusses the malignization of these states. (Tab. 2, Fig. 8, Ref. 16.)

Biopsy

Morphological changes of Neisseria gonorrhoeae in amniotic fluid of pregnant women in their midtrimester.

Ten selected samples of amniotic fluid obtained through transabdominal amniocentesis from 10 pregnant women in their 17th-19th weeks of pregnancy were investigated for the survival of Neisseria gonorrhoeae in amniotic fluid. In 8 cases an antibacterial effect was observed with morphological changes comparable to the effect of benzylpenicillin. When N. gonorrhoeae was inoculated in amniotic fluid at 10(5)-10(7) cells/ml, it survived as an average 2 h longer than after inoculation of 10(2)-10(4) cells/ml. Electron microscopic pictures of gonococci taken after 2.6 and 24 h incubation in amniotic fluid correlated with the growth curves. Electron microscopically there were marked morphologic changes of N. gonorrhoeae, viz. vacuolar degeneration of their cytoplasm with a damage to the bacterial wall up to its complete destruction and lysis of the cell.

Amniotic Fluid

[Review of the morphological changes in peripheral nerves in experimental diabetes mellitus].

A survey of morphological changes of myelinated nerve fibers of peripheral nerves recorded in experimental streptozotocin induced diabetes mellitus in rats is presented. Causes of discordant interpretations of findings are considered and pathogenetic mechanisms involved in morphological changes of myelinated nerve fibers of peripheral nerves observed in this model of diabetes mellitus are discussed. (Ref. 41.)

Animals

Light microscopy study of Schmidt-Lantermann incisures in the guinea pig sciatic nerve.

The authors studied the shape, orientation and number of Schmidt-Lantermann incisures in the sciatic nerve of the healthy guinea pig. The nerves were removed, fixed, shredded and evaluated in the light microscope. Internode length, fibre diameter and segment length were measured in isolated nerve fibres with an eyepiece micrometer. The number and configuration of the Schmidt-Lantermann incisures in the internode were found to be variable. The incisures follow one another at irregular intervals, dividing the whole internode into cylindroconical segments of varying lengths. The measured values were processed on a PMD 85/2 personal microcomputer.

Animals

Ultrastructure of the Schmidt-Lantermann incisures in the guinea pig sciatic nerve.

The authors studied Schmidt-Lantermann incisures in myelinated fibres of the guinea pig sciatic nerve. Incisures of different shapes were observed and described in longitudinal sections; some of them were simply shaped, but others had a very intricate form. It is assumed that the atypical, intricate forms are complete incisures which change course and acquire a complex shape in longitudinal sections.

Animals

[The effect of amniotic fluid on Neisseria gonorrhoeae].

This study shows the antibacterial effect of amniotic fluid collected on 10 women from 32 to 38 weeks. Two techniques were used. The first is a quantitative analysis of a bacterial culture with amniotic fluid as compared to controls, with inhibition by the amniotic fluid demonstrated. The second is a morphologic analysis of gonorrheal cultures by electron microscopy, showing alteration of the bacteria when amniotic fluid is present. These facts are of interest when one tries to understand the discrepancy between high rates of discovery of bacteria during pregnancy and low rates of intra-amniotic infection by Neisseria gonorrhoeae.

Amniotic Fluid