An outbreak of Norwalk-like virus infection in Tokyo and Saitama in late 1995.
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Biomedical subjects
Publications and source records attributed to K Bosu.
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Serotyping of human rotavirus in the Tokyo area was conducted from 1990 to 1993 by enzyme immunoassay with monoclonal antibodies (EIA-MAbs) against VP7 and by reverse transcription and polymerase chain reaction (RT-PCR) amplification of the VP4 and VP7 genes. The results by EIA-MAbs were very similar to those obtained by RT-PCR. Evidence of intraserotypic variations was suggested because strains of undetermined serotypes were detected by either EIA-MAb or RT-PCR. This kind of study is required for vaccine development.
A 9 month old boy with suspected rotavirus encephalitis developed infantile spasms and delayed psychomotor development. Anti-rotavirus antibodies in cerebrospinal fluid, blood, and faeces were studied.