PubMed Health⌕ Search

Biomedical subjects

K Brülhart

Publications and source records attributed to K Brülhart.

7 recordsLinked to original sources
PubMed1995

The number of circulating CD34+ blood cells predicts the colony-forming capacity of leukapheresis products in children.

In children, only a few guidelines are available for optimizing peripheral blood progenitor cell (PBPC) harvesting. We analyzed by means of flow cytometry and clonogenic assays 60 harvest products obtained from 20 children by standardized leukapheresis after treatment with chemotherapy and CSF. In addition, 27 fresh blood samples obtained prospectively during the mobilization phase were studied. CFU-GM/kg significantly correlated with MNC/kg, CD34+ cells/kg and CD34+33- cells/kg in apheresis products (P < 0.001). In fresh blood samples, CFU-GM/ml significantly correlated with MNC/ml, CD34+ cells/ml and CD34+33- cells/ml (P < 0.001). The numbers of CD34+ cells/ml, CD34+33- cells/ml and MNC/ml in 19 blood samples taken prior to leukapheresis were compared with CFU-GM/kg harvested and thawed after cryopreservation applying multiple regression analysis with stepwise variable selection. The number of circulating CD34+ cells/ml prior to leukapheresis highly correlated with and was predictive for the number of collected CFU-GM/kg (P < 0.001). In addition, a significant correlation (P < 0.05) between the number of progenitor cells/kg reinfused and the time to myeloid and platelet recovery was found in children undergoing high-dose therapy. Our data indicate that a single leukapheresis will be sufficient to obtain a minimum number of 5 x 10(4) CFU-GM/kg if the pre-harvest number of circulating CD34+ cells is > or = 10(5)/ml. Thus, our results will help to optimize PBPC transplantation in children.

Adolescent↗
PubMed1993

[Functional therapy of Achilles tendon rupture].

We are going to present two cases with functional treatment of Achilles tendon ruptures. We define a treatment as functional if the involved limb is either not immobilized at all or whenever the immobilization is incomplete and for a limited time only and when no operation is necessary. The functional treatment is presented as an alternative, and we are going to present the advantage and disadvantage.

Achilles Tendon↗
PubMed1988

[Surgery of congenital megaureter].

Despite the wide use of the international classification of megaureters, there is a lack of clear concepts especially for congenital malformations. We performed a follow-up study in 132 children with 171 megaureters operated on during the past 13 years. In 24 additional cases reconstructive surgery was not feasible due to advanced damaging of the parenchyma. In 97% of 171 megaureters, resection of the distal stenosis and ureterocystoneostomy was successful. In 20%, additional ureteric tailoring was necessary. Only one patient had recurrent reflux, and reoperation for recurrent stenosis was indicated in less than 2.5%. Urographic studies revealed that postoperatively 38% of calices resumed a normal appearance, whereas 80% of the ureters were of normal caliber and had normal peristaltic activity. From this study it is concluded that ureteric stenosis should be treated operatively in refluxing and obstructive cases. As a rule, correction of bladder or urethral pathology does not obviate the need for ureter surgery.

Child↗
PubMedDate not supplied

Pharmacokinetics and therapeutic study with nimesulide suppositories in children with post-operative pain and inflammation.

The pharmacokinetic pattern of 100 mg nimesulide administered rectally at different times prior to undergoing minor surgery was studied in 45 children. Absorption of nimesulide was relatively fast, a peak plasma concentration of 75 mg/l being reached 3 h after administration, and the elimination half-life was 3.15 h. The efficacy and tolerability of the nimesulide suppositories were assessed in a randomized, double-blind, dipyrone-controlled study of 50 children suffering from moderate to severe post-operative pain, the drugs being administered one to three times daily as required; 26 patients received nimesulide and 24 dipyrone. A consistent reduction in pain was recorded during nimesulide therapy given for a mean period of 2.5 days, with a mean consumption of 3.5 suppositories. Similar results were obtained in dipyrone-treated patients. The efficacy of both drugs was judged by the physicians to be good or very good in 70% of cases and there were no statistically significant differences between the two treatment groups in the dosage required or the pain relief. Tolerability of both drugs was excellent, with only one patient in each treatment group complaining of nausea.

Anti-Inflammatory Agents, Non-Steroidal↗