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Biomedical subjects

K C Bergmann

Publications and source records attributed to K C Bergmann.

At least 19 recordsLinked to original sources
PubMed1979

[Immunologic diagnosis].

The development of the immunologic basic research and a deeper insight into the pathogenesis of diseases with changed immune reactivity led to an intensivation of the clinic-immunologic in-vivo- and in-vitro-diagnostics. At the instance of the pulmonary diseases according to the classification of Gell and Coombs the immunologic-diagnostic possibilities and problems are shown. Here is referred to the importance of the use of standardized tests and of the wide replaceability of in-vivo-techniques by in-vitro-methods.

Anti-Glomerular Basement Membrane Disease↗
PubMed1979

[Evaluation of lymphocyte transformation test: relationship between nucleus and cell sizes of PHA-stimulated lymphocyes (author's transl)].

A methodological comparision of evaluation of lymphocyte transformation test is described. Three experts examined smear-slides of cells from five lymphocyte cultures and the corresponding controls in a blind study. In the morphological differentiation of 100 cells per slide in comparison to 1 000 cells per slide it was found a good intraindividually but a unsatisfactory interindividually correlation. 1 000 cells from stimulated and non-stimulated cultures shows the greatest difference for the parameter "total cell size" and the lowest difference for the "nucleus plasma relation" by the planimetrically imeasurement.

Cell Nucleus↗
PubMed1979

[Detection of antibodies against Aspergillus fumigatus. 1st report: making of antigens (author's transl)].

A method of antigen preparation from Aspergillus fumigatus has been described. We received the antigens from medium and mycel used for the detection of precipitins and IgE-antibodies. The antigenic components are demonstrated in the immunoelectrophoresis. By the use of absorption we did not find essential differences between medium antigen and mycel antigen for the detection of precipitins. The medium antigen recommended for diagnostic is easier to produce and, moreover, has advantages for the detection of specific IgE antibodies.

Antibodies, Fungal↗
PubMed1979

[Investigations for the use of micromodification of lymphocyte transformation test in patients with lung disease (author's transl)].

In a preliminary study a micromodification of lymphocyte transformation test was examined for use in patients with specific and nonspecific lung diseases. Investigation was carried out about the influence of different conditions in the cell culture system. A micromodification was defined and introduced in the clinic simultaneously to the conventional macromodification of lymphocyte transformation test. This micro lymphocyte transformation test is of use for determination of cell mediated immunity. However, in the described form it is doubtful for the detection of present specific sensibilization.

Dose-Response Relationship, Drug↗
PubMed1978

[Allergic alveolitis. Diagnosis and therapy in practice].

The diagnostic steps for the disease are listed and are discussed. The diagnosis includes: 1. the anamnesis, 2. the clinical examination, 3. the chest X-ray, 4. serological-immunological methods of examination including detection of antibodies, skin tests, allergen inhalation test, 5. lung function tests. The therapy consists in antigen exclusion and corticosteroids.

Adrenal Cortex Hormones↗
PubMed1978

[Frequency of lung function disturbances of industrial poultry farmers (author's transl)].

The frequency of antibodies against organic dusts of fowls were examined on 339 poultry farmers. 22% of workers had 1 antibody and 3% had 2 antibodies in the serum. The ventilation, the arterial oxygen tension, the residual volume, the Slope Index, the closing volume of each person were measured in the mobile laboratory. Signs of central obstruction and equal ventilation were found in sensitized persons (2 antibodies) about four times more than non-sensitized persons. Suitable lung function screening parameters were the FEV1/FVC and the Slope Index.

Allergens↗
PubMed1978

[Sensitivity and specifitivity of the counterimmunoelectrophoresis for determination of precipitating antibodies (author's transl)].

Counterimmunoelectrophoresis (C.I.E.) is suitable for routine-work in clinical medicine to give quantitative evidence of precipitating antibodies. Sensitivity of this method is high (lowest level being 200 to 400 ng albumin/ml serum) and range of evidence being very broad (14-15 titer steps). The direction of migrations of proteins with anodal migration (Ig) is changed by carbamylation. The experience gained in more than 3,000 investigations, concerning methods and evaluation of the results, is reported in detail. Counterimmunoelectrophoresis is superior to immunoelectrophoresis and double diffusion (Ouchterlony) due to its higher sensitivity and to RAST or RIST by reason of its lower costs.

Antigens↗
PubMed1978

[Allergy and lectins: action between IgE-mediated histamine release and glycoproteins from Viscum album L. (mistletoe)].

Low-molecular constituents from Viscum album (toxic) release, in irritatively toxic manner, histamine from human leukocytes without destroying the cells (cytotox test). This histamine release is prevented by preincubation with viscum lectins. The viscum lectin blocks the Fc-portion of allergen-specific IgE against anti-IgE [125I]. Lectin also inhibits the allergen-specific histamine release from human leukocytes occurring through IgE; this can be demonstrated on leukocytes from patients with atopic extrinsic asthma. The inhibitory effect of lectin could also be due to a primary membrane effect, in addition to blockage of IgE.

Glycoproteins↗
PubMed1977

[Immunoglobulin E--formation, determination and significance].

The detection of class E of immunoglobulins 10 years ago led to a decisive progress in the pathogenesis and diagnostics of allergic diseases. In the literary survey several essential findings concerning the structure of the IgE and concerning the quantitative and qualitative recognition of the total IgE and the allergen-specific freely circulating or cellularly fixed IgE-antibodies are cited. Diagnostically usable changes of concentrations of the level of total IgE are described.

Adolescent↗
PubMed1977

[Isolation and characterization of mistletoe extracts (Viscum album L.). II. Effect of agglutinating and cytotoxic fractions on mouse ascites tumor cells].

Lectin from mistletoe (Viscum album L.) was studied for its relations with the toxins from Viscum album, ascites tumor cells of mouse, and human immunoglobulins. Using affinity chromatography on glutaraldehyde-crosslinked IgG (human) from viscum crude extract, a fraction was isolated which exhibited full agglutination capacity and high toxicity. The supernatant showed no agglutination capacity but a strong toxic effect on mouse ascites tumor cells. This toxic effect could not be influenced by further additions of insolubilized IgG. Chromatography on DEAE cellulose also gave agglutinating fractions with toxic effects and a non-agglutinating toxic portion. Column chromatography on Sephadex G 75 allowed separation of toxic from agglutinating components. The molecular weight of the toxin remaining after lectin removal was above 10,000. Lectin was found to bind more readily to mouse ascites tumor cells than to erythrocytes.

Animals↗