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K C Hodde

Publications and source records attributed to K C Hodde.

11 recordsLinked to original sources
PubMed1990

Advances in corrosion casting methods.

This paper briefly discusses the concept of corrosion cast preparation (primarily of blood vessels), the use of the scanning electron microscope (SEM) to study these casts and the observations which can be made, together with the merits and the limitations in various applications. A number of reviews and surveys are quoted in which the different injection media, injection methods, animal preparations and corrosion procedures are described. A new procedure of cleaning the corrosion casts with sodium hydroxide and Triton X-100 is described. The observations which can be made are listed and illustrated both on the cellular level as well as in organ systems as a whole. The discussion centers around some common misconceptions, the feasibility in various applications and the limitations of the method. The conclusion is that the method has proven to be useful especially in conjunction with other methods. Moreover, while the concept of the method may be very straight-forward the approach and the interpretation often need careful consideration and might not be as straight-forward as one tends to expect from the simple sounding principle.

Animals↗
PubMed1990

The morphology of synovial lining of various structures in several species as observed with scanning electron microscopy.

Data concerning surface morphology of synovial lining of tendons, tendon sheaths, cruciate ligaments, infra-patellar fat pads and peripatellar synovial ridges in various species (rat, rabbit, dwarf goat, sheep, pig, dog, human) are reported on. Supportive studies with transmission electron microscopy (TEM) and light microscopy were performed. Three principal morphological appearances of the synovium are evident. On structures with a dense fibrous architecture like tendons, tendon sheaths and cruciate ligaments the intimal cells and processes are mostly slender and may tend to orientation in the length-axis of the structure. On the peri- and infrapatellar adipose tissues two principal 'extremes' are seen: one in which the contours of the fat cells are clearly visible with fungoid shaped structures in between them, and one in which the fat cell contours are not recognizable and the intima consists of cauliflower-like cells. Transitional forms exist. Several features observed on tendons and tendon sheaths which have not been reported on before are presented in this paper. A consistent classification of synovium is presented.

Animals↗
PubMed1987

Vascular corrosion casts of the dog testis after experimental microvascular surgical orchiopexy. A scanning electron microscope study.

Five mongrel dogs underwent microvascular surgical orchiopexy by anastomosing the testicular artery and vein with the respective caudal epigastric vessels. In order to assess the effect of a 40 to 50-min period of warm ischaemia (the time required to make the anastomosis) on the testicular blood vasculature, plastic corrosion casts were made of the operated testes and compared with non-operated control specimens. The resulting blood vessel casts were inspected in the scanning electron microscope. One case of testicular atrophy developed, caused by a postoperative wound abscess. Comparing operated with non-operated testes, no differences in vascularisation, pattern or density of arterial supply, capillary network and venous drainage were seen. These findings are in agreement with data from the literature concerning histological, hormonal and clinical results.

Anastomosis, Surgical↗
PubMed1987

Vasculature of the crista ampullaris of the rat as seen in microcorrosion casts.

A scanning electron microscope study of corrosion cast preparations of the vessels of the crista ampullaris was carried out in adult rats. This method shows the microvasculature of the cristae in the three-dimensional model. As a result of this technique we have been able to demonstrate on microscopical level a particular microvasculature in the septum cruciatum of the crista in rat.

Animals↗
PubMed1987

Effect of intracranial pressure on bridging veins in rats.

The behavior of bridging veins at their entrance into the superior sagittal sinus during elevated intracranial pressure (ICP) was investigated in rats using a cranial window and infusion of mock cerebrospinal fluid. The bridging veins became slightly smaller as the ICP rose (maximum reduction 17%). Compression or collapse of the veins was not observed, even at an ICP level of 100 mm Hg, and there was no cuffing of bridging veins upstream of the entrance. Both the scanning electron microscopic investigation based on resin vessel casts and the histological examination of whole-head coronal sections indicated that the narrowest points of the bridging veins are at their entrance to the superior sagittal sinus, caused by the oval aperture with thickened wall structure in the lateral sinus wall to which the bridging veins are connected. The present data thus support the concept that cerebral blood flow during intracranial hypertension is not reduced by venous cuffing or compression of the lateral lacunae and bridging veins; by contrast, the arteriovenous pressure difference seems to remain the determining factor even at high ICP. Thus, blood flow through the brain stops when ICP approaches the level of blood pressure.

Animals↗
PubMed1987

Vascular changes in popliteal lymph nodes due to antigen challenge in normal and lethally irradiated mice.

The microvascular system of the murine popliteal lymph node was investigated using scanning electron microscopy of microcorrosion casts. Time-dependent changes in the microvasculature following regional antigen challenge in normal and lymphocyte-depleted mice were studied. Normal lymph node microvasculature exhibited a significant increase in both the vascular bed and post-capillary venules containing high-endothelium in response to antigen challenge. Lymph nodes of lymphocyte-depleted mice showed no microvascular size increase following antigen challenge and a reduction in the amount of high-endothelium was observed.

Animals↗
PubMed1986

The angioarchitecture of the choroid plexus of the lateral ventricle of the rabbit. A scanning electron microscopic study of vascular corrosion casts.

The vascularization of the choroid plexus of the rabbit's lateral ventricle has been studied by scanning electron microscopy of vascular corrosion casts. Based on the vascular patterns discerned, the plexus was subdivided into 6 different parts: the inferior, lateral, central and superior parts, the free margin and the lingula. These differ in structure and localization. While the arterial supply of the inferior part is via the anterior choroidal artery, the central and superior part are supplied by the posterior choroidal arteries. Three main veins leave the plexus and drain to the basilar vein from the inferior part and to the internal cerebral vein from the inferior, central and superior part. Capillary diameters were typically 10-15 micron. Regular nodular thickenings up to 24 micron in diameter were seen in the free margin and the superior, lateral and inferior parts. 'Garland' shaped capillary configurations sheathing arteries as well as veins were found all over the plexus. It is suggested that these 6 different parts of the plexus described here may reflect functionally different areas.

Animals↗
PubMed1979

The vascularization of the pineal gland (epiphysis cerebri) of the rat.

The vascularization of the rat pineal gland was investigated with, respectively, latex and resin injection preparations of the cephalic blood vessels in adult Wistar rats. From the dissection of the latex-injected and aldehyde-fixed rat brains under the stereo light microscope it was found that the arterial supply comes exclusively from separate branches of the posterior cerebral artery. The pineal area was dissected out from the resin injected and corroded specimens under the stereo light microscope and was inspected in the SEM. The supplying arteries, identified on the basis of their endothelial cell imprint patterns, were 4 to 6 in number with a diameter of 20 to 40 micron. The venous drainage consisted of 12 to 16 short veins with a diameter of 40 to 60 micron, all draining directly into the great cerebral vein and thereafter via the sinus influens immediately into the systemic venous circulation.

Animals↗
PubMed1978

Scanning electron microscopy of the cochlear vasculature.

Studies on the vascularization of the cochlea with conventional injection methods that use light microscopy have failed in viewing both the overall three-dimensional picture, as well as the detailed construction of the cochlea vascular blood vessels. This basic shortcoming can be omitted by the use of scanning electron microscopy (SEM) on corrosion casts, which only presently has been introduced. The use of a commercial resin (Mercox) as an injected medium, followed by application of macerating fluids, provides clean and completely filled casts of the cochlea vascular system, which can then be inspected by SEM. This method not only confirms the general findings about cochlea vascularization but gives the opportunity to view the overall picture, as well as the detailed pattern of the intricate cochlear capillary beds.

Animals↗