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Biomedical subjects

K C Hong

Publications and source records attributed to K C Hong.

At least 19 recordsLinked to original sources
PubMed2005

Beta-lactoglobulin is a thermal marker in processed milk as studied by electrophoresis and circular dichroic spectra.

As much of the sterilization process involves heat treatment during the preparation of milk on an industrial scale, the unpredictable measures of the process are an essential issue in determining the quality of the milk. The purpose of the present study was to investigate the major protein change(s) of whey proteins in processed milk and extend the knowledge for future reference in the dairy industry. Using a native polyacrylamide gel electrophoresis, we showed almost a 90% loss and denaturation of beta-lactoglobulin (LG), but not alpha-lactalbumin (LA), in some brands of the processed and dry milks. Immunochemical analysis using Western blotting revealed that part of the loss was attributed to the formation of large multiple forms of LG in the processed product. Such denaturation was presumably associated with the heating procedure used in the process. Essentially, LG was the only major fraction converted to aggregates in milk heated at 95 degrees C for 30 min on 2-dimensional PAGE. The detailed thermal denaturation of purified LG and LA at various temperatures (50 to 95 degrees C) and time (5 to 960 s) were investigated using a circular dichroic analysis. The maximal changes of ellipticity at 205 nm (converting beta-structure to disordered structure) were correlated to heating temperature and time. There were no significant conformational changes of LG at temperatures below 70 degrees C for as long as 480 s. Pronounced and rapid changes occurred between 80 to 95 degrees C in a time-dependent manner. Fifty percent of the maximal changes could be reached within 15 s. In conclusion, the unique chemical and immunochemical loss and conformational changes made LG a superior marker for evaluating the thermal processing of milk. The detailed thermal denaturation curves of LG constructed with its time and temperature in this study provide a valuable reference for the dairy industry. We postulate that heat treatment over 80 degrees C in 15 s may induce a significant denaturation of milk LG.

Amino Acid Sequence↗
PubMed2001

Hepatocellular carcinoma with lung metastasis in a 9-year-old-boy.

Hepatocellular carcinoma (HCC) remains chemoresistant and, therefore, the principal treatment of HCC is surgical resection. After a 9-year-old boy with huge HCC with lung metastasis received the 5 cycles of chemotherapy (cisplatin and Adriamycin), the lung metastasis had been resolved completely and the size of HCC in liver had decreased. Right hepatic trisegmentectomy was performed, and then the additional 3 cycles of chemotherapy was given. Currently, the patient shows no recurrence of HCC 42 months after surgical extirpation.

Carcinoma, Hepatocellular↗
PubMed2001

Chronic hepatitis: in vivo proton MR spectroscopic evaluation of the liver and correlation with histopathologic findings.

PURPOSE: To correlate the in vivo hydrogen 1 ((1)H) magnetic resonance (MR) spectroscopic features of the chronic hepatitis-involved liver with the histopathologic stages of fibrosis. MATERIALS AND METHODS: Seventy-five patients with chronic hepatitis were examined with (1)H MR spectroscopy, which was performed in the right hepatic lobe. The peak areas of glutamine and glutamate complex (Glx), phosphomonoesters (PME), glycogen and glucose complex (Glyu), and lipid were measured on the liver spectra. The histopathologic features were correlated with the in vivo (1)H MR spectroscopic findings at each stage of chronic hepatitis. Fifteen healthy volunteers also were included as a control group. RESULTS: (1)H MR spectroscopy depicted Glx, PME, Glyu, and lipid in all livers. In the normal livers, the calculated mean (+/- SD) relative metabolite-to-lipid ratios of Glx, PME, and Glyu were 0.14 +/- 0.04, 0.03 +/- 0.01, and 0.21 +/- 0.04, respectively. The mean value of each metabolite-to-lipid ratio was significantly different between all stages of chronic hepatitis, and with the exception of the mean ratio at the interval between stages 0 and 1 (P > .05), the mean value increased significantly with increasing stage (P < .05). A pronounced peak was demonstrated at 3.9-4.1 ppm at (1)H MR spectroscopy of all stages of chronic hepatitis except stage 0. CONCLUSION: The increased Glx, PME, and Glyu levels relative to the lipid content with chronic hepatitis indicated the severity of fibrosis and thus were concordant with the histopathologic stages. In vivo (1)H MR spectroscopy might be a substitute for liver biopsy in the diagnosis and staging of chronic hepatitis.

Adolescent↗
PubMed2001

A case of hemorrhagic gallbladder paraganglioma causing acute cholecystitis.

Gallbladder paraganglioma is a very rare tumor and so far only a few cases have been reported. Most of these were asymptomatic and were found incidentally during operation. Recently, we experienced a gallbladder paraganglioma that gave rise to hemorrhage, which in turn caused acute cholecystitis. Our case involved a 45 year-old female patient complaining of an intermittent right upper abdominal pain. After a preoperative evaluation, cholecystectomy and lymphadenectomy were performed under the impression of gallbladder cancer with acute cholecystitis. Postoperative pathologic examination revealed a hemorrhagic gallbladder paraganglioma accompanied by acute cholecystitis. Immunohistochemical staining of the chief cells for neuron specific enolase, chromogranin and synaptophysin were positive. Sustentacular cells also stained positively for S100 protein.

Acute Disease↗
PubMed2000

A case of epidermoid cyst of the intrapancreatic accessory spleen.

A 54-year-old woman presented with a huge palpable mass on left upper quadrant of the abdomen. After preoperative work-up, a cystic disease of pancreatic tail or accessory spleen was initially suspected. We performed exploratory laparotomy and resected both the spleen and a 15 x 11 cm-sized huge cystic mass containing a part of solid component which extended continuously to the pancreatic tail. The solid component, comprising the upper portion of the resected cyst, was reddish brown and granular like as normal splenic tissue. The inner surface of the cyst was smooth and was filled with yellowish white material. Histologic examination showed an epidermoid cyst originating in the accessory spleen of the pancreatic tail lacking hair or skin appendages.

Epidermal Cyst↗
PubMed2000

Pneumatic colonic rupture accompanied by tension pneumoperitoneum.

Rupture of the colon caused by high pressure compressed air is a rare, unique and traumatic intra-abdominal injury. As the use of compressed air in industrial work has increased, so has the risk of associated pneumatic injuries from its improper use. Recently we experienced a case of pneumatic rupture of the sigmoid colon accompanied by tension pneumoperitoneum, which caused respiratory distress. The patient's respiration was very rapid with the rate of 44 breaths per minute. On arterial blood gas analysis, pH was 7.40, pO2 68 mmHg, pCO2 44 mmHg, and SaO2 90%. Chest X-ray film showed marked pneumoperitoneum and an elevated diaphragm. The respiratory distress was severe and required immediate relief by emergency decompression peritoneocentesis before surgical intervention consisting of the serosal tear repair, colonic rupture colostomy and abdominal cavity irrigation. A follow up operation 2 months later for colostomy repair completed the patient's recovery.

Adult↗
PubMed1999

Pancreaticoduodenal arcades and dorsal pancreatic artery: comparison of CT angiography with three-dimensional volume rendering, maximum intensity projection, and shaded-surface display.

OBJECTIVE: The objective of this study was to compare the ability of CT angiography to depict the pancreaticoduodenal arcades and the dorsal pancreatic artery using the techniques of three-dimensional (3D) volume rendering, maximum intensity projection (MIP), and shaded-surface display (SSD). SUBJECTS AND METHODS: Twenty-seven patients were selected at random from a group of 42 patients undergoing arterial-phase helical CT angiography before liver transplantation. CT angiograms were constructed from identical data sets using 3D volume rendering, MIP, and SSD. RESULTS: Seventy-two vessels were evaluated in 27 patients. Three-dimensional volume rendering depicted 24 anterior and 22 posterior arcades and 26 dorsal pancreatic arteries; combined MIP and SSD depicted 14 anterior and 13 posterior arcades and 19 dorsal pancreatic arteries. Thirty vessels with diameters of between 2 and 3 mm were well seen with 3D volume rendering but were incompletely depicted with MIP and SSD. Sixteen vessels with diameters of greater than 3 mm were well seen using all three techniques. Twenty-six vessels with diameters of less than 2 mm were faintly seen with 3D volume rendering but were unidentifiable with MIP and SSD. CONCLUSION: Three-dimensional volume rendering is superior to MIP and SSD in the depiction of pancreaticoduodenal arcades and dorsal pancreatic arteries. Unlike the other rendering techniques, 3D volume rendering can also show relationships between these vessels and pancreatic parenchyma and adjacent structures.

Adult↗
PubMed1999

Biliary lymphoepithelioma-like carcinoma not associated with Epstein-Barr virus.

We report the case of a 64-year-old man who presented with a hepatic mass and macronodular cirrhosis. The pathologic findings revealed a lymphoepithelioma-like carcinoma arising in the hepatobiliary tract that was morphologically identical to nasopharyngeal undifferentiated carcinoma. However, this tumor was not associated with Epstein-Barr virus infection in molecular studies. Macronodular cirrhosis associated with hepatitis C virus was present in the background liver.

Biliary Tract Neoplasms↗
PubMed1978

Changes in organic matrix of bone and of bone and blood ATP in rats fed rachitogenic diets.

Vitamin D deficient and phosphate deficient states were produced in young rats by the use of synthetic diets. Whole blood ATP, serum calcium and phosphorus and the ash, phospholipid, hexosamine and hydroxyproline contents of metaphyseal bones of control and rachitic rats were measured after 4 weeks on the diets. There was a decrease in whole blood ATP and serum phosphorus of the rachitic rats, and in the contents of ash, phospholipid and hexosamine of the rachitic metaphyses, while the hydroxyproline contents of the rachitic bones were higher than those of the controls. Subcutaneous injection of ATP or inorganic phosphate in rats, fed a low phosphate diet deficient in vitamin D, caused an increase in serum phosphorus and blood ATP levels and in metaphyseal ash contents. It is suggested that some of the metabolic disturbances in rickets are due to alteration in ATP production.

Adenosine Triphosphate↗
PubMed1976

Effect of estrogen on the collagenolytic activity of rat bone.

Rats were injected with 17 beta-estradiol benzoate intramuscularly and the collagenolytic activity of the rat bones was assayed by measuring the degradation of purified reconstituted 3H-proline- and 3H-hydroxyproline-labeled collagen. A diminution of the collagenolytic activity which could be recovered from the culture medium of estrogen-treated rat bone was observed. However, the addition of estrogen to the enzyme using the same assay revealed no diminution of collagenolytic activity. These results suggest that the diminution of collagenolytic activity in the culture medium of estrogen-treated rat bone is due to an alteration in the production or activation of collagenolytic activity.

Animals↗