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K Carson

Publications and source records attributed to K Carson.

25 records · Page 2Linked to original sources
PubMed1983

S-adenosylmethionine synthetase in cultured normal and oncogenically-transformed human and rat cells.

We have investigated the enzymatic formation of S-adenosylmethionine in extracts of a variety of normal and oncogenically-transformed human and rat cell lines which differ in their ability to grow in medium in which methionine is replaced by its immediate precursor homocysteine. We have localized the bulk of the S-adenosylmethionine synthetase activity to the post-mitochondrial supernatant. We show that in all cell lines a single kinetic species exists in a dialyzed extract with a Km for methionine of about 3-12 microM. In selected lines we have demonstrated a requirement for Mg2+ in addition to that needed to form the Mg X ATP complex for enzyme activity and have shown that the enzyme can be regulated by product feedback inhibition. Because we detect no differences in the enzymatic ability of these cell extracts to utilize methionine for S-adenosylmethionine formation in vitro, we suggest that the failure of oncogenically-transformed cell lines to grow in homocysteine medium may result from the decreased methionine pools in these cells or from the loss of ability of these cells to properly metabolize homocysteine, adenosine, or their cellular product S-adenosylhomocysteine.

Animals↗
PubMed1983

Behaviour of thoroughbred foals during nursing.

Thoroughbred foals were found to nurse in bouts of nursing activity delimited by intervals of non-nursing activity lasting 27 secs or longer. Nursing activity included nosing, sucking and interval behaviour. During the first week after birth, foals nursed, on average, seven times an hour with a mean bout duration of 147 secs but were not successful at sucking during all nursing bouts. Time spent nursing decreased as the foals grew older until before weaning, at 24 weeks of age, the foals were nursing once an hour with a mean bout duration of 74 secs. The dams hindered their foals' nursing activity in several ways during Weeks 2 and 3 post partum and it was suggested that at this time nursing was painful for the dams.

Animals↗
PubMedDate not supplied

The alcohol-fixed Diff-Quik stain. A novel rapid stain for the immediate interpretation of fine needle aspiration specimens.

A rapid alcohol-fixed Diff-Quik stain is described. The technique takes less than five minutes and results in excellent cytologic detail, comparable to that with Papanicolaou stain, as well as superb stromal definition. The stain should be very useful to pathologists and cytotechnologists for immediate interpretation of fine needle aspiration specimens.

Azure Stains↗