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Biomedical subjects

K Chang

Publications and source records attributed to K Chang.

At least 145 records · Page 8Linked to original sources
PubMed1983

Cell-mediated immune injury to the heart.

A micro in vitro procedure was utilized to assess cell-mediated immunity of patients following myocardial infarction or cardiac surgery. For this purpose, peripheral blood leukocytes from patients were tested in a microdroplet assay with antigen preparations derived from human cardiac tissue. Whereas whole saline extract and human myoglobin preparations had little effect on the migration of peripheral blood leukocytes in vitro, mitochondrial preparations were markedly effective in inhibiting migration of the leukocytes in the presence of mitochondrial extracts appeared to reflect development of cell mediated immunity to heart antigens after the myocardial infarct or surgery. These results extend the observations that humoral antibody may appear in patient following cardiac injury. The role of either antibody or sensitized lymphoid cells in mediation of post-myocardial infarction or post-cardiotomy syndromes is not clear, but it appears clear that injury to the heart induced auto-reactive responses which may play a role in subsequent pathologic events following the initial cardiac injury.

Adolescent↗
PubMed1983

Changes of serum protein and immunoglobulins in ovarian agenesis.

The levels of serum proteins and immunoglobulins were determined in five cases of Turner's syndrome, in five secondary amenorrheic patients who responded to progesterone, and in five healthy women as controls. Compared with the controls, the patients with secondary amenorrhea showed significantly lower albumin, but higher alpha 1- and beta-globulins; IgG was also lower. In the Turner's syndrome group, gamma globulin, IgG and IgM levels were significantly lower. These results support the suggestion that at least some of the serum proteins and immunoglobulins are related to estrogen.

Adolescent↗
PubMed1983

Feeding behavior induced by central norepinephrine injection is attenuated by discrete lesions in the hypothalamic paraventricular nucleus.

Extensive brain-cannula mapping studies in the rat have demonstrated that the hypothalamic paraventricular nucleus (PVN) is the most sensitive brain site for eliciting eating behavior with central norepinephrine (NE) injection. The present experiments examined the impact of lesions aimed at the PVN on this NE-elicited eating response. In rats with NE injection cannulas aimed at the lateral ventricle, bilateral lesions of the PVN significantly attenuated, by 60 to 70%, the eating effect induced by NE, at doses ranging from 20 to 160 nmoles. PVN lesions which extended ventrally to damage tissue lying within the periventricular region were more effective in abolishing the NE response than were lesions that remained confined to the dorsal aspects of the PVN. Large lesions located just dorsal to the PVN had no impact on the NE response. This evidence supports the primary role of the PVN in mediating the eating behavior elicited by central noradrenergic activation.

Animals↗
PubMed1983

Anatomic and clinical analysis of 96 Beall prostheses explanted over a 13-year period.

Over 13 years, 96 Beall prostheses (80 Teflon, 16 Pyrolite) explanted at surgery were examined for durability, material wear, and clinical symptoms leading to surgical indication for excision were evaluated. The Teflon valves showed progressive disc wear and notching and two cases of disc escape from the cage; the Pyrolite discs were intact in all prostheses. The Dacron-Velour covering common to both types showed dehiscence at the inlet, significantly more severe after the third implant year in the Teflon (p less than 0.005) than in the Pyrolite prostheses. Sixty-nine (85 percent) cases with Teflon valves showed clinical manifestations of congestive heart failure due to prosthetic dysfunction; in 50 there was significant hemolytic anemia. Seven (44 percent) of the 16 Pyrolite disc valves were excised primarily because of dysfunction. The degree of hemolysis with the Pyrolite valves was usually mild. These data clearly suggested the advisability of early excision of the Beall Teflon prostheses in symptomatic patients and a close follow-up in patients with the Pyrolite models.

Adult↗
PubMed1983

Campylobacter hyointestinalis (new species) isolated from swine with lesions of proliferative ileitis.

Intestines from 48 swine with enteric disease were examined by bacteriologic cultural technique for the presence of various Campylobacter species. Histopathologic techniques were used to determine whether the submitted specimens had lesions of either swine proliferative ileitis or other enteric diseases. Three species of Campylobacter were identified as Campylobacter jejuni/coli, Campylobacter sputorum ss mucosalis, and Campylobacter hyointestinalis (proposed new species) on the basis of biochemical characteristics and response to various inhibitory substances. The C hyointestinalis was isolated from 18 of 27 (67%) swine with proliferative ileitis and from only 1 of 21 (5%) swine with other enteric diseases. The C sputorum ss mucosalis was obtained from 16 of 27 (59%) swine with proliferative ileitis and from 2 of 21 (10%) swine with other enteric disease. The C jejuni/coli was isolated from 2 of 27 (7%) swine with proliferative ileitis and from 8 of 21 (38%) swine with other enteric disease. The new organism, C hyointestinalis, was catalase-positive, hydrogen sulfide positive in triple sugar iron agar, glycine tolerant, intolerant to 3.0% sodium chloride, able to grow at 25 C, sensitive to cephalothin, and resistant to nalidixic acid. On the basis of these characteristics, C hyointestinalis was differentiated from other campylobacters isolated from swine and from other sources.

Animals↗
PubMed1982

Hemofiltration treatment of psoriasis.

Eleven patients with severe psoriasis were treated with hemofiltration once weekly for 4 consecutive weeks. Hemofiltration is a method of extracorporeal dialysis that uses a pressure gradient, rather than passive diffusion, to remove small and medium molecular weight substances from the blood. Six of the patients had very substantial clearing of their psoriatic lesions. Five patients had little or no apparent benefit during the course of hemofiltration treatments. They subsequently had dramatic clearing, however, when previously ineffective topical therapy was resumed. No patient had worsening of his condition during the hemofiltration series, and there were no perceived adverse effects.

Adult↗
PubMed1980

Increased collagen cross-linkages in experimental diabetes: reversal by beta-aminopropionitrile and D-penicillamine.

The effects of diabetes on collagen cross-link formation and solubility were investigated in granulation tissue collagen induced by polyester fabric implanted subcutaneously in rats at the same time diabetes was produced by injection of streptozotocin. Thus, all the collagen analyzed was formed in a diabetic milieu. Ten days later the implants were removed and the total collagen content as well as the fraction soluble in 0.5 M acetic acid was determined. Predominantly type I collagen accumulated in the implants. Total collagen content was the same in diabetics and controls; however, the acid-soluble fraction in diabetic animals was only half that of controls (8.5% and 17.7%, respectively), and the ratio of beta chains to alpha chains in the acid-soluble fraction was higher in diabetics (0.89) than in controls (0.69). In animals treated with beta-aminopropionitrile or D-penicillamine the acid-soluble fraction of collagen from diabetics equaled that from controls. These observations indicate that both intramolecular and intermolecular cross-links are increased in type I collagen from diabetic animals. Since these cross-links interfere with degradation of collagen by collagenase, they may contribute to accelerated intimal sclerosis of arteries and to capillary basement membrane thickening in diabetes.

Amino Acids↗
PubMed1979

Cardiac specific antigen and antibody in immunopathogenesis of cardiac disease.

Antibody to human cardiac extracts was detected in the sera of many patients undergoing open heart surgery. A passive hemagglutination assay using cardiac extracts as antigen coated onto erythrocytes showed the development of cardiac antibody in the patients, with peak titers usually one to three weeks after the surgery. Radioimmunoassay revealed the presence of cardiac specific myoglobin antigen antigen in the sera of many patients, with peak levels 1-3 days after the procedure. A correlation was observed between development of circulating serum antibody and appearance of cardiac myoglobin antigen. These results support the view that immunologic disease related to release of cardiac antigen in patients undergoing heart surgery may be a factor in post-cardiotomy disease.

Antibodies↗
PubMed1979

Effect of heat on the circular dichroism of spectrin in hereditary pyropoikilocytosis.

Hereditary pyropikilocytosis is a hemolytic anemia in which the erythrocytes show increased sensitivity to heat-induced fragmentation. Circular dichroism measurements were employed to study the effect of heat on the secondary structure of pyropoikilocyte membrane proteins. The magnitude of the ellipticity at 222 nm over the temperature range from 25 degrees to 70 degrees C was determined for erythrocyte ghosts, spectrin, and ghost residue after extraction. In pyropoikilocyte ghosts, protein denaturation began at a lower temperature and the midpoint of the structural transition was displaced from 49 degrees C (the value for normal ghosts) to 44 degrees C. This thermal transition was present in spectrin, but not in the ghost residue after extraction. We conclude that an abnormality in the spectrin molecule alters the physical and morphologic properties of the erythrocyte membrane in pyropoikilocytosis.

Anemia, Hemolytic, Congenital↗