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Biomedical subjects

K Connor

Publications and source records attributed to K Connor.

23 records · Page 2Linked to original sources

The synthesis and accumulation of cholesteryl esters by the developing embryo of the domestic fowl.

A comparative study has been made of the accumulation and synthesis of cholesteryl esters in the liver, yolk sac membrane, and yolk contents of the chick embryo during the last week of development. This involved a comparison of the cholesteryl esters synthesized during incubation in vitro with 14C-cholesterol with those accumulated during incubation in vivo. Results indicated that the considerable accumulation of cholesteryl esters, mainly cholesteryl oleate, that occurred within the liver during embryo development arose from synthesis in the yolk sac membrane. This suggested a specific role in the transport of yolk lipid into the embryo. With the approach of hatching the ability of the liver to synthesize its own cholesteryl esters increased while that of the yolk sac membrane decreased.

Animals

Mode of action of phosphonoformate as an anti-herpes simplex virus agent.

Phosphonoformate inhibited the replication of Herpes simplex virus (HSV) type 1 and type 2 in culture. The concentration required to inhibit the replication of both types of virus by 2 logs at 28 h post-infection was approximately 150 microM. It was more potent than phosphonoacetate against the growth of both virus types. A virus mutant which is resistant to phosphonoacetate was cross-resistant to phosphonoformate. Arsonoacetate, at 300 microM, had no antivirus activity. Phosphonoformate also inhibited HeLa and KB cell growth; at a concentration of about 500 microM, cell growth was inhibited by 50%. The anti-cell growth effects of the drug were completely reversible. The antivirus effect of phosphonoformate was partially reversible, depending on the time and duration of exposure of infected cultures to the drug. To obtain the maximum antivirus effect, phosphonoformate had to be added within the first 3 h post-virus-infection and be continuously present for at least 18 h. Phosphonoformate, added at 0 h post-infection, suppressed the induction of virus-specific DNA polymerase and DNAase activities. dTMP incorporation into DNA was preferentially inhibited in nuclei isolated from infected cells compared to uninfected cells, and the degree of inhibition varied with the ionic strength of the assay. Phosphonoformate was a potent inhibitor of the purified HSV-1 and HSV-2 DNA polymerases, inhibiting DNA polymerase activity by 50% at a concentration of 3 microM and ionic strength of 0.2.

Antiviral Agents

Understanding visual metaphor: developmental and individual differences.

The development of an instrument--the Metaphoric Triads Task (MTT)--for the assessment of metaphoric comprehension is described. In the tradition of earlier cognitive-style research, a visual triad format was adopted that offered three possible pairings of pictorial stimuli, one of which was metaphorical in character. A subject's score reflects the number of metaphoric pairings formed (with appropriate metaphoric explanations) across all of the triads of the task. Data are reported for 12 samples of subjects (ranging from 7 1/2 to 28 years of age) who responded to the MTT in a diverse array of studies. Internal analyses of the MTT yielded satisfactory reliabilities (interjudge and internal consistency) and item-sum correlations. Sex differences were negligible, but progressive improvement in MTT score with age was noted. At the same time, a slight modification of the MTT triad format generated performance levels from younger children that approximated those of children 1--3 years older who had taken the MTT in its standard form. Higher MTT scores were generally obtained by those subjects who attempted more pairings, spent more time at the task, and chose the metaphoric pair as "best" among the alternative pairing possibilities. Correlations of MTT performance with standardized tests of intellective aptitudes and achievements were inconsistent across samples and between the sexes within samples. In contrast, MTT scores were quite consistently related to solving difficult analogies, generating high-quality responses to divergent-thinking tasks, and manifesting broad categorizing and physiognomic sensitivity. Significant correlations between the MTT and a set of verbal metaphoric triads offered convergent validational evidence suggestive of a general metaphoric style. Some relation was found between MTT performance and teacher ratings of figurative language appreciation and esthetic sensitivity, though it appeared that these might be mediated by the teachers' estimate of the child's overall capability. Finally, three experimental training studies were carried out. A requirement of exhaustive pairing and informative feedback on pretest items significantly enhanced the MTT performance of the younger children. The provision of appropriate verbal labels for each picture in a triad also significantly enhanced performance by insuring that children's encoding of the pictures was consistent with the metaphoric linkage in each item.

Adolescent

Pharmacologic treatment of catheter-related thrombus in pediatrics.

Venous and arterial catheters are being used with increasing frequency in pediatric populations. As part of the comprehensive management of these catheters, nurses need to be aware of the more common catheter-related thrombus problems. An understanding of pharmacologic treatment using Streptokinase and Urokinase for thrombus in central lines, hemodialysis grafts, and umbilical artery catheters can help nurses develop protocols to care for patients with these devices. The appropriate use of thrombolytic therapy can restore function to many catheters thereby saving the patient the cost and morbidity associated with removal and replacement of a dysfunctional catheter.

Catheters, Indwelling