PubMed Health⌕ Search

Biomedical subjects

K Cuperlović

Publications and source records attributed to K Cuperlović.

17 recordsLinked to original sources
PubMed1992

[Susceptibility and the immune response to Trichinella spiralis infection].

The aim of this study was to assess a possible association between parameters of immune response and susceptibility/resistance to T. spiralis infection. The immune response to T. spiralis was monitored in two inbred strains of mice (BALB/c and C57Bl/6), as well as in the most important natural host--swine, by analysing the changes in main lymphocyte populations, the appearance of specific antibodies and worm burden recovery. In the murine model, the lower level of muscle larvae worm burden recovery in mice expressed greater resistance (BALB/c) was associated with manifested clinical signs, earlier development of humoral antibody response and a persistently higher level of CD4/CD8 T cell ratio, compared to T. spiralis-susceptible mice (C57Bl/6). In swine, high susceptibility to infection was also associated with lack of clinical symptoms, a delayed development of an antibody response and persistently low CD4/CD8 T cell ratio.

Animals↗
PubMed1990

Peripheral blood mononuclear cell subsets during Trichinella spiralis infection in pigs.

The immune response of 'Yugoslav meat breed' pigs inoculated with low doses of Trichinella spiralis muscle larvae was followed over two to nine weeks of primary infection, by analysing changes in peripheral blood mononuclear cell subsets, the development of a humoral antibody response and muscle larvae burden. During the course of the infection, infected animals showed a persistent elevation of both CD4+ and CD8+ T cell subsets from days 15 to 60 after the parasite exposure. During this time, the number of peripheral blood mononuclear cells expressing major histocompatibility complex class II antigens was also increased, while no significant differences were found in the number of circulating monocytes/macrophages and B cells over time. Humoral antibody responses to muscle larvae excretory-secretory products were evident as early as 41 days after infection, while the muscle larvae were recovered as early as 27 days after infection. The increased levels of CD4+ and CD8+ T cell subsets, as well as cells expressing major histocompatibility complex class II antigens in pigs exposed to T spiralis, may be indicative of some considerable alterations in cell subsets that are involved in the regulation of the swine immune response to this parasite.

Animals↗
PubMed1989

Comparative efficacy of antigen and antibody detection tests for human trichinellosis.

Sera collected from patients with suspected or confirmed exposure to Trichinella spiralis were tested for circulating parasite antigens and antiparasite antibodies. Using an immunoradiometric assay, excretory--secretory antigens from muscle-stage larvae of T. spiralis were detected in the sera of 47% of 62 patients with clinical trichinellosis and 13% of 39 patients without clinical signs but suspected of exposure to infected meat. In comparison, antibodies were detected using an indirect immunofluorescent test in the circulation of 100% of the 62 patients with clinical trichinellosis and 46% of the 39 patients with suspected exposure. The presence of antibodies specific to excretory-secretory products of T. spiralis muscle larvae was confirmed in the majority of the samples tested by a monoclonal antibody-based competitive inhibition assay. These results indicate that antibody detection is a more sensitive diagnostic method for human trichinellosis, but that antigen detection might be a useful confirmatory test because it is a direct demonstration of parasite products in the circulation.

Antibodies, Helminth↗
PubMed1977

[Diagnosis of von Willebrand's disease].

Diagnosis and differential diagnosis of von Willebrand's disease was a special problem. Criteria up to date: prolonged bleeding time, reduced platelet adhesiveness, decreased F. VIII coagulant activity, as well as a particularly behaviour after the infusion of F. VIII, were not sufficient to differentiatie this disease from other congenital disorder of F. VIII. Recent investigations, introducing the immunological methods for determination of F. VIII--related antigen, as well as the investigation of ristocetin induced platelet aggregation, give the new approach in the diagnosis of von Willebrand's disease. Authors presented preliminary results of investigations of F. VIII--related antigen in the patients with von Willebrand's disease, as well as the results of investigation of the patients with hemophilia A and normal subjects, as a control group.

Adult↗
PubMed1977

[Immunoelectrophoretic method for the determination of factor VIII-related antigens].

Immunological examination of F VIII related antigen gave some new information on the nature of congenital defects of F VIII, in haemophilia A and von Willenrand's disease. Besides classic method in diagnostics of von Willebrand's disease, the determination of F VIII related antigen can be used as a diagnostic criterium in distinguishing von Willebrand's disease from some mild forms of haemophilis, as well as in detection of haemophilia carrier. In addition, the study on the relationship of immunological value of F VIII related antigen and biological activity of F VIII offers more data on the possibility of detection of so-called "hypercoagulability" and states proceeding thrombosis. The method for determination of F VIII related antigen (Rocket electrophoresis--Laurel) as well as the values of F VIII in health persons of our population is described in this paper.

Antigens↗
PubMedDate not supplied

Immunoglobulin levels in seminal plasma of oligospermic patients.

The levels of immunoglobulins in the seminal plasma of healthy males were compared with those of oligospermic patients. The results show markedly increased levels of IgG in oligospermic patients. The presence of IgM, which has not been detected in the seminal plasma of healthy males, was estimated in some of the oligospermic patients. The authors recommend this simple screening test to be carried out in every male infertility follow up.

Autoimmune Diseases↗