PubMed Health⌕ Search

Biomedical subjects

K D Flossmann

Publications and source records attributed to K D Flossmann.

At least 37 records · Page 2Linked to original sources
PubMed1980

[Problems relating to the use of radioactively labelled bacteria in animal experiments. 3. Distribution of activities in mice, following parenteral administration of Pasteurella multocida labeled with various radionuclides].

When radioactively labelled fully developed Pasteurella (P.) multocida antigens were parenterally applied to mouse, through intravenous, intraperitoneal or subcutaneous routes, activity distribution was found to depend both the technique of application and the radionuclide used. This has given rise to the conclusion that activity distribution could be equated with whole-germ distribution only under certain conditions. Experimental antigen distribution, accordingly, reflected a complex process in which transport, location, and metabolism of bacteria had to be considered as being in conflict with the given host organism. Iodine labelled 125 was split off bacteria at relatively high rate and accumulated in the thyroid gland. When 59Fe was used, interpretation of distribution was rendered more difficult by propagation of iron through the organs of the host. Relatively favourable data were obtainable from the use of 3H or 14C which could be used in the form of nucleic acid precursors (orotic acid) in labelling Pasteurella. Intraperitoneal and intravenous forms of administration were found to be the same for intra-organic activity distribution. Retention of activity in liver, spleen, and lung was recordable and established from all the reviewed techniques for the application of P. multocida complete antigen. While maximum concentrations were measured between the first and third days, activities still were detectable after 14 days (period under review). Relatively high radioactivity was recordable from the digestive tract, within short time from the use of the parenteral procedures tested. This seemed to suggest passage of complete or incomplete P. multocida antigen through the mucous membranes of the gastro-intestinal tract.

Animals↗
PubMed1979

[Deoxyribonucleic acids from Pasteurella multocida and Pasteurella haemolytica].

Pasteurella multocida and P. haemolytica strains contain between 1.5 and three per cent phosphorus, between nine and 14 per cent nitrogen, between two and four per cent DNA, and between five and 18 per cent RNA, the precise figures depending on culturing conditions. High-molecular DNA may be isolated by means of bacteriolysis, using deoxycholate or dodecylsulphate and the usual steps of purification, with yield and purity differing by strains. DNA with sufficient purity can be obtained from Sepharose 2 B by gel chromatography. The isolated DNA yields were characterised, base values being between 37 and 38 per cent GC for P. haemolytica and between 41 and 48 per cent GC for P. multocida. Highly suitable precursors to DNA synthesis for tritium labelling are 3H-thymidine, which is incorporated in excess of 3H-thymine by a factor of 255, as well as 3H-uracil, with its activity being recovered also from the pyrimidine bases of DNA via pyrimidine biosynthesis.

Chromatography, Gel↗
PubMed1977

[Chemically defined and partially defined media for pasteurella multocida and pateurella hemolytica].

Two culturing recipes were suggested for Pasteurella multocida and Pasteurella haemolytica, one partialy defined nutritive defined nutritive medium with 0.5 per cent yeast extract and one chemically defined medium. Both had been composed on the basis of studies into nutrient demand and into the effects of complex nutritive substrates. Both nutritive media are applicable also to culturing other bacterial species. (Among the strains tested so far were streptococci, staphylococci, Escherichia coli, pseudomonas, protest, Salmonella,, Shigella, and Haemophilus.)

Bacteriological Techniques↗
PubMed1977

[Problems in the use of radioactively labelled bacteria in experiments. 2. Quantitative method for the evaluation of pathogens in the calf lung by means of labelled bacteria after aerosol or intratracheal administration].

By means of two aerosol apparatures radioactive labelled Pasteurella-multocida-germs were given to calves via a respiratory mask. Labelling of Pasteurellae was done internal by using special nutritive media with Fe-59 or P-32. In a period of 10-25 minutes 5 X 10(9)-10(10) labelled bacteria were given to the animals for inhalation. Another animal group was injected intratrachealy with the same quantity of labelled germs. Immediately after termination of germ application the animals were killed and dissected. After solution or homogenization, respectively of the total lung tissue radioactivity was determined by liquid-scintillation-counting (Fe-59) or Cerenkov-measurement (P-32), respectively. The activities recovered from lung homogenates rendered is possible to calculate the received amount of bacteria. 40-80% of germs would be recovered in the lung after application by intratracheal injection while only 1-7% could be recovered after individual aerosol application lasting for 15-25 minutes.

Aerosols↗
PubMed1977

[Problems in the use of radioactively marked bacteria in animal experiments. 1. Labeling of Pasteurella multocida, Pasteurella haemolytica and Salmonella dublin with eH, 14C, 32P, 59Fe, 99mTc, 125J1].

Several methods are suggested by which to use the radionuclides 3H, 14C, 32P, 59Fe, 99mTc, and 125J for labelling or doublelabelling of Pasteurella multocida, Pasteurella haemolytica, and Salmonella dublin, with particular reference being made to labelling ofr animal experiments. Suitable radioactive substrates for internal labelling in chemically defined or partially defined nutritive media include 3H-thymin, 3H-thymidine, 14C-glucose, 14C-mannose, 14C-aspartic acid, as well as 3H-uracil, 3H-uridine, 3H-orotic acid, 14C-orotic acid, 59Fe-III-citrate or chloride, and Na2H32PO4. The choise of the nuclide and substrate should by governed by the problem at hand.

Isotope Labeling↗
PubMed1977

[Studies on calf salmonellosis. 3. Preclinical evaluation of a Smd. Salmonella dublin live vaccine].

Results of preclinical assay, preparation and conservation of a S.-dublin live vaccine based on streptomycin dependent mutants and for oral application are demonstrated and discussed in detail. The used oral vaccine is very good tolerated by calves and results in stable immunity after administration of a daily dose of 5 x 10(10) bis 1 x 10(11) living Smd.-mutants for 10 consecutive days. Complete immunity developes in calves within a period of two weeks after the last antigen administration and persists up to the age of 5 to 6 months. Conserving the vaccine at low temperatures (-15 degrees C) the number of living organism is far-reaching preserved in the first 5 months after preparation. After thawing the vaccine is to be used within a period of 3 days. With respect to the preparation of the live vaccine on semisynthetic nutritiv media some informations are given concerning the improvement of bacterial yield.

Administration, Oral↗
PubMed1977

[Binding relationships of fatty acids in lipid A of lipopolysaccharides from Pasteurella multocida].

Each glucosamine disaccharide of lipid A from Pasteurella multocida contains 2 mols myristic acid and 4 mols hydroxymyristic acid. 3-hydroxymyristic acid was identified as fatty acid with amide linkage. Every 2 mols of glucosamine have linked to them 1 mol myristic acid, 3-hydroxymyristic acid, and 3-myristoxymyristic acid. The results so far obtained reflected the structure of lipid A of enterobacteriaceae.

Binding Sites↗
PubMed1976

[Nutrient demand of erysipelothrix bacteria].

Studies into the nutrient demand of Erysipelothrix insidiosa led to the preparation of a nutritive medium with high bacterial yield which is recommended for quantity breeding of bacteria. The medium was complex and did not contain serum but the following components: peptone S, yeast extract, glucose, Na2HPO4, arginine, and Tween 80.

Animals↗
PubMed1976

[Culture media for Salmonella dublin on a chemically defined basis].

The nutrient demand of S-dublin strains was investigated. Media on chemically defined basis for culturing of S. dublin are recommended with reference to the above studies. The definition includes Na2HPO4, KH2PO4, NaCl, glucose, the amino acids L-asparaginic acid, L-cystine, L-glutamic acid, serine, and L-threonine, and nicotinamide as vitamin component. Such media will be quite favourable for certain culturing problems, but complex substrate (yeast extract, peptone, tryptone) should be added for high yields.

Culture Media↗