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Biomedical subjects

K D Hanna

Publications and source records attributed to K D Hanna.

14 recordsLinked to original sources
PubMed1993

Keratotomy for astigmatism using an arcuate keratome.

We describe a new system for performing transverse arcuate corneal incisions for the correction of astigmatism. The instrument consists of a conical housing with suction and/or friction fixation to the limbus and two micrometric diamond knives mounted on a carrier that can be rotated by a gear mechanism. The depth, length, and location of the incisions can be predetermined. Light microscopy of donor eye corneas documented the achieved incision depth. One sweep at normal speed produced an incision depth 28% less deep than the preset depth. One sweep at low speed achieved depths of approximately 100% of the knife setting. Variability from one incision to another in the same cornea was related to blade sharpness. We report successful use of the instrument in five consecutive eyes with naturally occurring astigmatism.

Adolescent↗
PubMed1993

Synthetic epikeratoplasty in rhesus monkeys with human type IV collagen.

Human type IV collagen discs were found to support proliferation and adhesion of rabbit corneal epithelial cells in tissue culture. To assess the biocompatibility of this synthetic collagen for epikeratoplasty, seven eyes of seven rhesus monkeys underwent epikeratoplasty with lenticules made of human type IV collagen. Eye rubbing by the animals expulsed two of the lenticules and caused failure of two to epithelialize completely. The remaining three lenticules epithelialized, remained clear, and caused no adverse effects on the eye. Two of these lenticules developed focal areas of subepithelial thinning 3 months postoperatively and the third lenticule has remained stable for 30 months. The presence of epithelial attachment components at the epithelial-lenticule interface was demonstrated by immunolocalization. Histopathologic and ultrastructural examination revealed focal areas of epithelial invasion and degradation of the lenticule. Neutral proteases were detected in the thinning region of one specimen. Human type IV collagen supports epithelialization in vivo and may have potential as a biomaterial for epikeratoplasty, but the stability of the material must be improved.

Animals↗
PubMed1992

Corneal wound healing in monkeys after repeated excimer laser photorefractive keratectomy.

Five rhesus monkey eyes underwent repeated argon fluoride (193 nm) excimer laser myopic photorefractive keratectomy 3 months following an initial ablation that had produced mild subepithelial haze. At 3 months all eyes had development of a dense subepithelial opacity and a thickened epithelium (12 cells, 80 microns) with vacuolization of basal cells, fragmented basement membrane, and a layer of subepithelial fibrosis containing activated fibroblasts. By 6 months the opacity was clearing; epithelium was thinner (50 microns); subepithelial fibrosis was more lamellar. By 15 months only mild haze persisted clinically; epithelium was 30 microns thick, with persistent basal vacuolization and focal basement membrane disruption; subepithelial fibrous tissue was more organized. Early repeated excimer laser ablation of the monkey cornea apparently induces vigorous stromal wound healing. Use of shallower ablations, corticosteroids, or a longer delay between ablations may be necessary for repeated laser surgery to be practical clinically.

Animals↗
PubMed1990

Wound healing after excimer laser keratomileusis (photorefractive keratectomy) in monkeys.

Laser myopic keratomileusis (photorefractive keratectomy) was performed on 29 rhesus monkey corneas with an argon fluoride (193-nm) excimer laser and a computer-controlled, moving slit delivery system. The 4-mm-diameter central ablation zone ranged in depth from 11 microns (-2 diopters effect) to 46 microns (-8 diopters effect). Corneas were studied for the 9 months postoperatively by clinical slit-lamp microscopy, and periodically with light and transmission electron microscopy. By 6 weeks, mild to moderate subepithelial haze was apparent in 93% of the corneas, with considerable variability in density. Progressive clearing occurred so that by 6 to 9 months 12 of 13 surviving corneas (92%) were either completely clear (4 corneas) or trace hazy (8 corneas). The epithelium was thickened at 21 days after ablation and returned to normal thickness by 3 months. At 3 weeks, subepithelial fibroblasts were three times the density of normal keratocytes and returned to nearly normal numbers by 9 months. We concluded that the anterior monkey cornea demonstrated a mild, typical wound healing response after excimer laser keratomileusis.

Animals↗
PubMed1989

Corneal stromal wound healing in rabbits after 193-nm excimer laser surface ablation.

An argon fluoride excimer laser (193 nm) with a moving slit delivery system was used to perform anterior myopic keratomileusis in both eyes of 24 New Zealand white rabbits. Rabbits were killed immediately after ablation and at intervals up to 100 days. By slit-lamp microscopy, four rabbits at day 100 exhibited four clear corneas and four corneas had central, spotty, subepithelial haze. Light and electron microscopy documented corneal healing. In the early stages a transient acellular zone in the anterior stroma appeared over a period of three weeks, followed by an increased number of fibrocytes. In the corneas with opacification, focal areas of 20-microns-thick subepithelial scarring were present. An unexpected finding was transient damage to posterior stromal keratocytes and endothelial cells. The endothelium produced a layer of granular material that migrated anteriorly across Descemet's membrane. Immunochemistry at day 6 showed a marked staining for collagen IV, proteoglycans, fibronectin, and laminin.

Animals↗
PubMed1989

Preliminary computer simulation of the effects of radial keratotomy.

We created a computerized mathematical model of the eye for the simulation of refractive surgery. This model used a slightly prolate shape for a moderately myopic eye and an idealized aspheric profile for the cornea. The finite element method and the nonlinear Mooney-Rivelin law were used to analyze stress-strain relationships. Radial keratotomy changes stress distribution in the cornea and the anterior sclera, with major changes at the paracentral and peripheral ends of the incisions. The model predicted that there would be a flattening of the central cornea with a posterior displacement, which increased with an increase in intraocular pressure. A change in the length of the incision of 0.5 mm produced a significant change in correction. For small clear zones (3 to 4 mm), incisions of equal length produced comparable changes in refraction. We found that the effect of the corneal radius of curvature on the amount of refractive change was negligible. We studied the effect of elasticity coefficients and corneal anisotropia.

Biomechanical Phenomena↗
PubMed1989

The Hanna suction punch block and trephine system for penetrating keratoplasty.

We describe a new system for corneal trephination. The system for cutting donor buttons consists of a concave Teflon well in which the donor corneoscleral shell is secured by gentle suction. A cylindrical guide ensures that the disposable razor blade trephine is centered and is perpendicular during punching from the endothelium. The suction mechanical trephine used for the host has the following features: (1) a gun-sight alignment system for reliable centering, (2) a 360 degrees limbal suction ring that secures the trephine to the eye and ensures perpendicularity of the cut, (3) support surfaces on either side of the disposable razor blade that support the cornea during cutting to create near-vertical uniform wound margins, (4) rotation of the trephine with a manually operated gear system for smooth cutting, and (5) a calibration device that governs the extension of the blade and also allows cutting without descent of the blade.

Corneal Transplantation↗
PubMed1989

Scanning slit delivery system.

We describe the structure and function of a delivery system designed for use with an argon fluoride excimer laser for corneal surgery. The basic principle of the delivery system is to shape the laser beam with a slit mask to a defined configuration. The image of the slit is moved across the cornea in a mathematically defined pattern to ablate a given amount of tissue, creating a new anterior radius of curvature for the cornea. The location of the beam on the cornea is determined by a rotating dove prism and a translating spherical lens, each of which is controlled by computer-regulated servomotors. The system is versatile and can be used for laser myopic or hyperopic keratomileusis, for correction of astigmatism by surface ablation, for creation of radial or transverse linear cuts, for excision of corneal buttons, and for uniform lamellar corneal smoothing procedures. The delivery system is currently used in the laboratory for the ablation of nonhuman primate corneas.

Animals↗
PubMed1989

Computer simulation of arcuate and radial incisions involving the corneoscleral limbus.

We have created a computer model of the eye that improves upon previous models with two assumptions: (1) the cornea is considered anisotropic, and (2) the strain tensor is non-linear for large displacements. Values used include those for Young's modulus, Poisson's ratio, and three coefficients of anisotropia. A finite element computer program was used to stimulate the behaviour of the eye. Four simulations were done: (1) a full thickness arcuate limbal cataract incision, (2) a partial thickness arcuate corneal relaxing incision, (3) a partial thickness radial incision extending across the limbus, (4) a partial thickness radial incision confined to the cornea. A full thickness corneal arcuate incision for cataract induced more change in corneal shape than a limbal incision. However, the gaping is more important in the limbal incision. Partial thickness arcuate corneal relaxing incisions were found to be more effective with a clear zone 3-5 mm in diameter. Radial incision simulation demonstrated little enhancement of the effect of the incisions when across the limbus.

Astigmatism↗
PubMed1988

Excimer laser keratectomy for myopia with a rotating-slit delivery system.

We performed argon fluoride excimer laser (193-nm) superficial keratectomy for myopia on human donor eyes and on a resected corneal disc. The laser beam was shaped by a rotating slit to produce a circular ablation 7.5 mm in diameter, with a mathematically defined profile to correct myopia. The fluence at the surface of the cornea was 200 mJ/cm2; the laser was fired at 20 Hz. Each 4.5-mJ laser pulse etched a 0.17-micron deep image of the slit in the cornea. Since the slit moved (0.03 Hz), each successive pulse etched an area adjacent to the previous one, reducing damage from repetitive pulses striking the same area. The slit scanned the cornea many times and the summation of these individual ablations produced the smooth myopic ablation profile, as shown by computerized keratographs and light and electron microscopy.

Cornea↗
PubMedDate not supplied

Computer simulation of arcuate keratotomy for astigmatism.

BACKGROUND: The development of refractive corneal surgery involves numerous attempts to isolate the effect of individual factors on surgical outcome. Computer simulation of refractive keratotomy allows the surgeon to alter variables of the technique and to isolate the effect of specific factors independent of other factors, something that cannot easily be done in any of the currently available experimental models. METHODS: We used the finite element numerical method to construct a mathematical model of the eye. The model analyzed stress-strain relationships in the normal corneoscleral shell and after astigmatic surgery. The model made the following assumptions: an axisymmetric eye, an idealized aspheric anterior corneal surface, transversal isotropy of the cornea, nonlinear strain tensor for large displacements, and near incompressibility of the corneoscleral shell. The eye was assumed to be fixed at the level of the optic nerve. The model described the acute elastic response of the eye to corneal surgery. RESULTS: We analyzed the effect of paired transverse arcuate corneal incisions for the correction of astigmatism. We evaluated the following incision variables and their effect on change in curvature of the incised and unincised meridians: length (longer, more steepening of unincised meridian), distance from the center of the cornea (farther, less flattening of incised meridian), depth (deeper, more effect), and the initial amount of astigmatism (small effect). CONCLUSIONS: Our finite element computer model gives reasonably accurate information about the relative effects of different surgical variables, and demonstrates the feasibility of using nonlinear, anisotropic assumptions in the construction of such a computer model. Comparison of these computer-generated results to clinically achieved results may help refine the computer model.

Animals↗
PubMedDate not supplied

Lamellar keratoplasty with the Barraquer microkeratome.

Lamellar keratoplasty was performed in 17 eyes: 6 with corneal opacities following epidemic keratoconjunctivitis, 3 with herpes keratitis, 2 with anterior corneal dystrophies, and 6 others. The Barraquer microkeratome was used for the resection in both the host and the donor. A technique is described to correct major disparities in diameter between the bed and the donor disc. In eyes with vision limited only by corneal diseases (11), the best postoperative spectacle corrected visual acuity was 20/25; the mean postoperative spectacle corrected visual acuity for the 11 eyes was 20/40.

Adolescent↗
PubMedDate not supplied

Corneal wound healing in monkeys 18 months after excimer laser photorefractive keratectomy.

Excimer laser keratomileusis (photorefractive keratectomy, direct corneal ablation) for myopic corrections of 2.00 diopters (n = 1), 4.00 D (n = 4), and 8.00 D (n = 3) was performed on eight corneas of four Rhesus monkeys. All animals were followed for 18 months. The ablations healed normally and no epithelial erosions occurred. Serial slit-lamp microscope examinations revealed that a variable amount of corneal haze developed in all animals; this haze progressively faded during the follow-up period. Histopathology revealed an epithelium of normal thickness, basement membrane abnormalities, increased number and activity of stromal keratocytes, and a variable amount of newly secreted extracellular matrix in the anterior stroma. These findings suggest that excimer laser keratomileusis induces a mild wound healing response in the anterior cornea which displays considerable individual variability and persists up to 18 months.

Animals↗