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K D Pettigrew

Publications and source records attributed to K D Pettigrew.

60 records · Page 4Linked to original sources
PubMed1971

Lecithin-agar assay for lecithinase antibodies in serum.

A technique for assay of lecithinase antibodies in serum was developed in this laboratory by using a lecithin-agar plate diffusion procedure based on a combination of described plate assays. Egg yolk lipoprotein composed primarily of lecithin was used as a substrate for reaction with free or non-neutralized lecithinase C after incubation of known amounts of lecithinase C with various dilutions of control and test sera. It was found that the size of the reaction zone was a function of enzyme concentration and inversely proportional to the antibody concentration. Accuracy and precision of the assay were determined. In addition, lecithinase antibody levels in sera from experimentally inoculated rats and rabbits and sera from randomly selected human patients were studied.

Agar↗
PubMedDate not supplied

Cerebrovascular integrity in protein-deprived rats.

Protein-deprivation does not increase the permeability of the blood-brain barrier in rats aged 3.5--12 months. PA, the product of cerebrovascular permeability (P) to 14C-sucrose and of cerebral capillary surface area (A), is very low in mature rats that have been maintained either on an 8% or 25% casein diet, and equals about 8 X 10(-6) sec-1 in both groups. There is a tendency for the calculated distribution volume of 14C-sucrose within the brain to decline in protein-deprived rats. Conscious, partially immobilized, protein-deprived rats have the same mean blood pressure, heart rate, arterial plasma pH and adreno-sympathetic response to stress (as measured by plasma norepinephrine and epinephrine concentrations) as do normally fed animals.

Animals↗
PubMedDate not supplied

Detection of an Alzheimer disease pattern of cerebral metabolism in Down syndrome.

Correlational analysis of cerebral metabolic (rCMRglc) data obtained with positron emission tomography (PET) assesses group differences and has demonstrated reduced frontal-parietal rCMRglc interdependencies in Alzheimer's disease (AD). A multivariate analysis of rCMRglc data assesses individual differences. We recently identified discriminant functions, reflecting frontal-parietal rCMRglc interdependencies, that separated AD from control subjects. To test if the functions would identify an AD rCMRglc pattern in older Down syndrome (DS) adults with (DS DAT+) or without (DS DAT-) dementia, we applied the functions to longitudinal rCMRglc data in: young DS (n = 15), DS DAT- (n = 10), DS DAT+ (n = 4), and young controls (n = 15). All DS DAT+ and some of the later DS DAT- scans were classified as AD. The results provide additional validation of the functions and suggest their utility for the early detection of AD.

Adult↗
PubMedDate not supplied

Pattern of cerebral metabolic interactions in a subject with isolated amnesia at risk for Alzheimer's disease: a longitudinal evaluation.

A pattern of reduced cerebral metabolic rate for glucose (rCMRglc) has been shown by positron emission tomography (PET) in patients with dementia of the Alzheimer type. To verify if a similar rCMRglc pattern is present in subjects 'at risk' for Alzheimer's disease (AD), we used high-resolution PET to longitudinally study a subject with isolated memory impairment and a family history for autosomal dominant AD. Initial rCMRglc data did not reveal any consistent abnormality as compared to a group of sex- and age-matched healthy controls. However, 1 year later, a follow-up evaluation did reveal reduced parietal rCMRglc values coinciding with a worsening of cognitive impairment, which suggested that standard analyses of resting rCMRglc data may not be useful in the early diagnosis of AD. In contrast, when a previously determined discriminant function for distinguishing controls from AD patients was applied, the subject was correctly identified as an AD patient on both PET scans.

Aged↗