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K Dörries

Publications and source records attributed to K Dörries.

24 records · Page 2Linked to original sources
PubMed1988

DNA rearrangements in organ-specific variants of polyomavirus JC strain GS.

Variants of JC virus (JCV) strain GS were isolated directly from the central nervous system (variant GS/B) and the kidney (variant GS/K) of a patient with progressive multifocal leukoencephalopathy and were cloned and sequenced. The genomes of the isolates were shown to be nearly identical in the nucleotide sequences of their protein-coding regions, suggesting that both had originated from a single infecting JCV genome. In contrast, the arrangement of the putative elements of transcriptional control revealed considerable differences. The tandemly repeated elements found twice within the enhancer region of JCV GS/B variant were not present in the GS/K variant. The missing elements were replaced by DNA segments containing simian virus 40 and adenovirus E1A core enhancer elements. These differences in the organ-specific GS variants suggest that rearrangements within elements of transcriptional control might be involved in altering the virus-cell interaction in the course of a JCV infection.

Base Sequence↗
PubMed1987

Association of polyomaviruses JC, SV40, and BK with human brain tumors.

Human brain tumors of 11 different types were analyzed by Southern blot analysis for the presence of JCV, SV40, and BKV. In 21 tumor specimens examined with JCV- and SV40-specific probes no positive hybridizations were obtained. Analysis for BKV DNA, however, revealed the presence of BKV-specific sequences in 11 of 24 tumor specimens. No hybridization was found in DNA from CNS tissues from different areas of 29 individuals without CNS tumors. The BKV DNA sequences were associated with high molecular weight cellular DNA, suggesting a chromosomal location. These data provide evidence for the involvement of BKV in the development of human brain tumors.

BK Virus↗
PubMed1984

Progressive multifocal leucoencephalopathy: analysis of JC virus DNA from brain and kidney tissue.

JC virus-specific DNA from both brain and kidney of the PML case G.S. was analysed by restriction mapping and Southern blot analysis. The genome of JCV DNA from brain (JCV GS-B) was full length, whereas JCV DNA GS from kidney (JCV GS-K) was 120 bp smaller. Restriction maps, constructed from JCV GS-B using various enzymes showed that most cleavage sites were consistent with standard map sites. Comparison of virus DNA from brain and kidney revealed that JCV GS-specific cleavage sites were identical, and that the DNAs were therefore of the same subtype. The deletion in kidney DNA was situated in the area of the origin of replication which is known to be hypervariable in the JCV genome. Restriction maps revealed that the DNA from each organ was homogeneous in length but a proportion of the DNA molecules were heterogeneous in restriction sites. It is concluded from these data that initial infection with one JCV subtype was followed by the development of heterogeneous DNA molecules.

Brain↗
PubMed1983

Progressive multifocal leucoencephalopathy: detection of papovavirus JC in kidney tissue.

Cellular DNA of the kidney from a patient with PML was analyzed by reassociation kinetics for the presence of JC virus DNA. Various amounts of viral DNA sequences were detected in different areas of the kidney. The highest concentration (175 genome equivalents/cell) was found in the renal medulla and there were almost none in the renal cortex. Differentiation from the closely related BK virus was carried out by reassociation kinetics and restriction enzyme cleavage with subsequent Southern blot analysis. The enzyme Hind II, which does not cleave within the BK virus genome, generated four restriction enzyme fragments in the cellular DNA from the kidney, thus documenting the presence of JC virus DNA. By examination of the renal DNA with the "no-cut" restriction enzyme XHO I and the "one-cut" enzymes Eco RI and BAM HI it was possible to show that free and not integrated viral DNA was present in these cells. Nonhomogeneous defective DNA bands were not detectable. By in situ hybridization the epithelial cells lining the collecting tubules were found as predominant site of the viral infection in the kidney.

BK Virus↗
PubMed1979

Detection of polyoma virus DNA in PML-brain tissue by (in situ) hybridization.

The human papova (JC) virus was extracted from brain of a patient with progressive multifocal leukoencephalopathy. A single band of virus was obtained at a density of 1.345 g/ml CsCl.JC virus DNA was purified and a highly specific cRNA was generated in vitro. In situ hybridization with JC virus cRNA and autoradiography on sections of the same brain revealed silver grains over oligodendrocytes, astrocytes and possible vascular endothelial cells, indicating the presence of JC virus DNA in these different cell classes.

Astrocytes↗