PubMed Health⌕ Search

Biomedical subjects

K Dĕdic

Publications and source records attributed to K Dĕdic.

18 recordsLinked to original sources
PubMed2006

[Isolated lymphadenopathy as the first presentation of systemic mastocytosis--description of two cases].

Presented are two cases of systemic mastocytosis in 46- and 63-year-old women, where the correct diagnosis was established in randomly disclosed cervical respectively intraabdominal lymphadenopathy. Both cases lacked characteristic skin and systemic mast-cell mediator symptoms at the time of histologic diagnosis. The first case was classified as a indolent systemic mastocytosis without any proven genetic alteration, the second one met the criteria of aggressive systemic mastocytosis with eosinophilia, where the point mutation asp816val in c-kit gene was confirmed and the patient responded unexpectedly well to Gleevec. Discussed are both conventional morphological differential diagnosis of mastocytosis in lymph nodes and recent advances in genetics of these systemic clonal mast cell proliferations. The latter not only outlines the oncopathogenesis but, in particular, also provides important prognostic and biological implications of this peculiar disease.

Abdomen↗
PubMed2004

[Bone marrow fibrosis during therapy of hairy cell leukemia].

The influence of Cladribin therapy to bone marrow fibrosis in patients with hairy cell leukemia was studied. Eighteen patients were included in the study; bone marrow fibrosis was graded 0-4 according to the quantity and pattern of distribution of reticulin. The grade of bone marrow fibrosis was established before the therapy and then in 12- and 24-month intervals. Patients showed complete remission after the therapy and remarkable reduction of bone marrow fibrosis.

Aged↗
PubMed2004

[Complete regression of bone marrow fibrosis following allogeneic peripheral blood stem cell transplantation in a patient with idiopathic myelofibrosis].

The authors present a case of a 47-year-old man with idiopathic myelofibrosis. The diagnosis of myelofibrosis was made in 1981. Because of progression of the disease and failure of hematopoiesis in 2002, allogeneic peripheral blood stem cell transplantation was performed; the donor was an HLA identical relative. Six months after transplantation, trephin biopsies were made and a complete regression of bone marrow fibrosis was documented. It is the first case of this treatment for idiopathic myelofibrosis in the University Hospital in Hradec Králové.

Adult↗
PubMed2003

[Immunohistochemical detection and quantification of minimal residual disease using DBA.44 antibodies in trephination biopsies of hairy cell leukemia].

Hairy cell leukemia patients treated with 2-chlorodeoxyadenosine or 2-deoxycoformicin achieve a very high number of therapeutic responses. After complete remission, i.e. minimal residual disease, we cannot demonstrate the disease activity, splenomegaly, or lymphadenopathy; moreover, there comes to normalization of hemoglobin concentration, leukocyte count, and platelet count. No leukemic cells in peripheral blood or bone marrow smears can be seen with the use of staining techniques. They can be demonstrated immunohistochemically in decalcified trephine bone marrow biopsies with the use of DBA.44 antibody together with their morphologic features. For quantification of leukemic cells we use LUCIA-M image analysis.

Antibodies, Monoclonal↗
PubMed2002

[Hairy-cell leukemia].

Presented is on overview of hairy cell leukemia problems in diagnostics. Methods of diagnosis--both clinical (clinical course, laboratory findings) and histopathologic are described in detail. There are also immunophenotypic findings described. Furthermore, differential diagnosis together with therapeutic methods, and the prognosis of the disease are depicted, too.

Diagnosis, Differential↗
PubMed2000

[Monitoring minimal residual disease in patients with hairy cell leukemia in complete remission after treatment with 2-chlorodioxyadenosine].

Treatment of hairy cell leukemia with 2-chlorodeoxyadenosine (2-CdA) induces in 85% patients complete remission. Complete remission is defined as the condition when signs of activity of the disease are absent, splenomegaly and lymphadenopathy are absent, the hemoglobin concentration is > or = 120 g/l, the absolute number of granulocytes is > or = 1.5 x 10(9)/l and the number of thrombocytes is > or = 100 x 10(9)/l. In complete remission in the peripheral blood, bone marrow aspirate and bioptic samples obtained by trephin bone marrow core biopsy, using standard staining (hematoxylin-eosin and May-Grünwald-Giemsa's method), no leukemic cells are present. When more sensitive methods are used (immunophenotyping, immunohistochemistry or molecular genetic methods), a persisting leukemic population can be detected which is described as minimal residual disease (MRD). For detection of MRD the authors used immunohistochemical examination of bone marrow with DBA.44 antibodies. As leukemic cells they described those which produced intense cytoplasmic and membrane positivity with antibody DBA.44 and corresponded morphologically to hairy cells. For evaluation computer analysis of the picture LUCIA-M was used. The infiltration grade was examined on three areas of standard size (3 x 65,265 micron 2) and expressed in percent. A total of 45 trepanobioptic specimens from 21 patients were examined who achieved after treatment with 2-CdA complete remission. In all samples suitable for evaluation the presence of leukemic cells (MRD) was detected with a median of 3% and a range of 1% to 18%. With induction of complete remission correlates also the low serum level of the soluble receptor for IL-2 (sIL-2R). In a female patient after 24 months of treatment with 2-CdA the grade of leukemic infiltration rose from 1% to 12% and during the 36th month to 50% DBA.44+ leukemic cells. The incipient relapse in this patient was not associated, despite marked infiltration of bone marrow, with failure of hematopoiesis and a marked rise of sIL-2R.

Adult↗
PubMed1999

Minimal residual disease, its detection and significance in hairy-cell leukemia.

As minimal residual disease (MRD) is considered the detection of hairy cells (HCs) in a patient with hairy cell leukemia (HCL) in complete remission with the absence of detectable HCs by routine morphology of peripheral blood, aspirates and bone marrow core sections, using more sensitive methods of identification as immunohistological staining or polymerase chain reaction (PCR) to detect immunoglobulin heavy chain genes rearrangement. Various monoclonal antibodies (MoAbs) as CD20, DBA.44, B ly-7, HC2, CD25 and CD11c have been applied using immunological staining. There is no standardized technique for identification of MRD. According to the technique used the MRD has been detected in 13% to 100% of patients in complete remission (CR). It may be concluded that many patients, if not all, in stable CR may have residual HCs. Whether MRD will have impact on early relapse or on long term outcome, or whether patients in CR with persistent MRD will remain so, is a matter of a longer follow-up.

Antibodies, Monoclonal↗
PubMed1999

[Gastrointestinal stromal tumor--GIST--case report].

Authors describe a newly introduced oncological entity that represents different views on some already well known mesenchymal and neuroectodermal tumours of gastrointestinal tract. These submucosal GIT tumours, showing only few differences in both the clinical and histological pictures, are generally called gastrointestinal stromal tumours. This paper presents a case of 37 year old patient with this disease.

Adult↗
PubMed1998

[Synovial metaplasia around breast implants].

Three cases of the periprosthetic breast capsules showing typical features of synovial metaplasia are presented. The histological picture is characterized by the presence of cellular layer mimicking synovial membrane. These elements are devoid of basement membrane, otherwise, nevertheless, they strongly resemble single- or multilayered epithelium. Focally, the differentiation towards giant multinucleated cells is noted. Immunohistochemical profile, as well as the ultrastructure of the cells, confirms their histiocytic origin with advanced differentiation towards elements of the synovial membrane. These histological features are referred to as synovial or synovial-like metaplasia. They are found in considerable fraction of textured breast implant capsules. The pathogenesis remains uncertain; however, similar picture was observed in other locations in association with prolonged or repeated action of mechanical forces. Thus, movement of the implant in situ is the most probable trigger of synovial metaplasia.

Adult↗
PubMed1998

[Pathology on the Internet].

Authors' experience gained during a one year usage of the Internet is presented. By now we have found many useful information resources related to the field of pathology. The MEDLINE database is available free of charge at several web sites as well as teaching diagnostic seminars, electronic color atlases, medical publishers homepages, etc. There is also a possibility to enter various topic-related groups in the framework of Internet discussion groups. The limiting factors for reaching medical information from the Internet is hardware and software equipment, the cost of Internet connection, and the data transmission capacity of phone lines.

Computer Communication Networks↗
PubMed1997

[Transmyocardial laser revascularization--histopathologic findings].

Findings in a 56-year old man who died 3 months after laser heart surgery were described. In the site of surgery (anterior and lateral wall of left heart ventricle) patent slit-like channels we found that may or may not have been performed by laser. Channels were indistinguishable from lymphatics. In second case, a 59-year old woman died 5-days after laser revascularization. Channels filled by fibrin, cellular debris and polymorphonuclear leukocytes were found, none of them patent.

Coronary Disease↗
PubMed1997

Long-term results in hairy cell leukemia treated with 2-chlorodeoxyadenosine.

We treated 19 patients with hairy cell leukemia (HCL) with 2-chlorodeoxyadenosine. 15 patients followed up at least 6 months were evaluated. The follow up period varied between 6 months and 37 months (median, 19 months). 8 patients were previously treated. The overall response in 15 evaluable HCL patients was 100%, with 87% complete hematological remissions including three patients with retroperitoneal and mediastinal lymphadenopathy and one patient with leukemic infiltrates of the cornea: 13% of patients achieved partial hematological remission. Soluble interleukin-2 receptor (sIL-2R) considered as a reliable non-invasive marker of HCL tumor burden dropped from the median of 1350 pM/ml (range 188 to 9000 pM/ml) to the median of 84.3 pM/ml (range 37 to 382 pM/ml) RdW which reflects the anisocytosis of red cells decreased after therapy from the median of 20.6% (range 13.1-25.0%) to the median of 13.7% (range 12.4-16.3%).

Adult↗
PubMed1993

Mixed medullary-follicular and medullary-papillary carcinoma of the thyroid: one or two entities?

Among 36 cases of medullary carcinoma of the thyroid we found two cases of mixed medullary-follicular carcinoma which in addition to the typical morphology contained foci of clear cells with cytological features of papillary carcinoma of the thyroid. The authors compare the two cases with the recently described entity called medullary-papillary carcinoma of thyroid.

Adenocarcinoma, Follicular↗
PubMed1993

Inoculation of C6 cell suspension into the brain of adult rats: immunohistochemical study.

C6 glioma cell suspension has been inoculated into the brain of adult Long Evans rats. Animals were allowed to survive 2 to 60 days and then immunohistochemical detection of S100 protein and glial fibrillary acidic protein (GFAP) in tumors was carried out on paraffin-embedded sections. In our in vivo model the maximum positivity for both S 100 protein and GFAP was observed in C6 glial cells at 10 days after implantation. At that time increased levels of S 100 protein were expressed both in central areas containing more differentiated C6 glioma cells and in host reactive astrocytes at tumor boundary. Almost no S 100 protein was found in dividing and invading, i.e. less differentiated, C6 glioma cells at tumor periphery and in perivascular spaces of adjacent blood vessels. The distribution of GFAP positive cells followed a similar pattern as that of S 100 protein containing C6 cells. GFAP expressing cells were revealed in quiescent central tumor portions which were occupied by more differentiated cells. Tumors were surrounded by strongly GFAP positive host reactive astrocytes. Later on, when signs of tumor regression appeared there was a decrease in S 100 protein and GFAP immunoreactivity of C6 glioma cells. To summarize, we developed an in vivo model for observation of cell differentiation within a growing glioma. Less differentiated and more malignant glioma cells expressed almost no S 100 protein and GFAP in contradistinction to central and more quiescent tumor portions.

Animals↗
PubMed1992

Embryonal form of extraskeletal myxoid chondrosarcoma with intermediate filament positive hyaline-like globules.

Reported is a case of an embryonal form of extraskeletal myxoid chondrosarcoma. The tumor cells contained PAS-positive, eccentrically located intracytoplasmatic, hyaline-like globules. These globules seemed to be a hallmark of this tumor, and they reacted strongly with cytokeratin and S-100 protein antibodies. The tumor was negative in reactions with desmin and GFAP antibody.

Biomarkers, Tumor↗
PubMed1990

[Granulomatous processes in the lungs].

Findings of lung granulomas are not rare but their etiology may be embarrassing in autoptic and especially in bioptic material which is shown in two such cases: a 19-year old drug addict man practising intravenous administration of Alnagon pills and a 54-year old woman working with acrylonitrile fibres. Differential diagnosis of granulomas is discussed at large.

Acrylonitrile↗