Biomedical subjects

K DiBiase

Publications and source records attributed to K DiBiase.

3 recordsLinked to original sources

PubMed1985

Characterization of a Q subregion gene in the murine major histocompatibility complex.

We have used restriction enzyme digests, Southern blot analysis, and gene transfer experiments to identify a class I gene in the Q subregion of the murine major histocompatibility complex. By comparisons of class I genes from Q congeneic strains, five restriction fragment length polymorphisms were identified. Further studies of mutant (Qa-2-) and wild-type (Qa-2+) BALB/c sublines indicated that at least part of the structural or regulatory gene controlling a Q subregion antigen resides on a 3.7-kilobase Xba I DNA fragment and is absent in all tested Qa-2- strains. The spontaneously occurring Qa-2- BALB/cBy mutant appears to have an extensive deletion in this region. The identity of this gene was confirmed by gene transfer experiments as well as by the use of a single-copy probe.

Animals↗

PubMed1982

Supramolecular relationships of membrane antigens on the murine thymocyte.

The topographical relationships of Thy-1, Ly-1, Ly-2, and T200 were examined on the murine thymocyte. The inhibition of the binding of radiolabelled monoclonal antibody after incubation with unlabelled heterologous antibody was used as a measure of the proximity of the target antigens on the cell surface. On unfixed cells, prior incubation with anti-Thy-1.2 impeded the attachment of labelled anti-Ly-1, anti-Ly-2, and anti-T200. Anti-T200, but not anti-Ly-1, or anti-Ly-2, was capable of impeding the attachment of labelled anti-Thy-1.2 on unfixed cells. In general, gentle fixation with paraformaldehyde did not alter these inter-relationships. The possible functional contributions of such supramolecular relationships are discussed.

Animals↗

PubMed1981

New complexities at the Qa-1 locus.

Mouse strain and tissue distribution analyses indicate that the new antiserum A anti-A-Tlab recognizes the cell-surface product governed by the previously serologically undetectable Qa-1b allele. This cell-surface product has therefore been called Qa-1.2. Three levels of anti-Qa-1.2 cytotoxicity in the presence of complement have been observed: high, intermediate, and zero lysis. In general, high levels of lysis correlate with the presence of the Qa-1b allele, while zero levels of lysis correlate with the presence of the Qa-1a allele. The A.CA strain reacts with both anti-Qa-1.1 and anti-Qa-1.2 and may possess a third allele, Qa-Id. Several strains including B6-H-2k react in an intermediate fashion. Recombinant strain analyses indicate that this intermediate reaction may be due to modifying genes within the H-2D region.

Alleles↗